XB-CLONE-3915977
Clone Name: IMAGE:7091142
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Gene:
slc25a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653651 [+]
taattaatccccccccccccccccccgctctgttagccggtgagggggctagtgaacgccatcttgttggttgccgccgaaatgtattcctccgttgtgc
agttagcgcggaccaaccctttcaatgctccccactttcagttggggcaagattctgtaatatcaaggaaaggcaatatcccccaaacaaagccgacccg
tcagcttgctgctgctgctgttcaagaagcaggtgaattcagttgtgaatatggctcaggaacgttttatgcatattgtggctttggtggcatcctgagt
tgtggtctaacccacactgctgtcgtaccactggatttggtcaaatgtcgtcttcaggttgaccctcaaaaatacaagagcatcttcaatggcttttcca
ttacccttaaagaagatggtgttcgtggccttgcaaagggatgggctccaacgttcattggatattcaatgcagggcctgtgcaagtttggtttctatga
agtgttcaagatactgtatgctaatatgctgggcgaggaaaattcctacatgtggcgtacttctctttacctggctgcgtctgccagtgctgagttcttt
gctgatattgctctggctccaatggaagctgcaaaagtacgcattcagacacaaccaggctatgctaaa
3' EST: CN324253 [+]ttttttttttttttgtgtttgggatggatatttagttctgtacattaaatctttaaccatcagtttgttaagtttaaattacataaatgcaataactctc
atctgtttatttcaacagataaatacagcggtaaaaccaagttacaatgtgtaggctatgcggcaaggtgagagcacaaccaataattacatctgaaaca
accataggtcctattttttagttgaatttataaaaaacgccttttgaaaatgttggagtttgcatgatttgagtccatgtttactgagtgagaccaagct
tcttcttcaaggattctggcatctcagggggaggaggacgaggaagtctgaagtaaaccttcacagagtcatagataaaccactgcaaagcagtcaaggt
accaatcatgatgatacgggcagtcaaacccttccagacacctttaggcccaagtctcttcagaacttccaaagcagtgcttcccttttccttgttcagt
acagagaccacagaatccgcaggatgtgagacaatggcacagaagacaccagcaatatatccagccacgaaagtgacaaccaactgctcagattttgaac
attcgcttctgggtttgggcacaacatacttatagagagcttcaacagttcgttcaaagcaagcaaatttcatcattgtgtatggaatctgcctcatcca
aagaggagcaacaccttttgtaaaacgcccagatgccttcctcagcatacatcttgggagcagctttgtcttaaagtattagcaatacctgggtggtgtc
tgaaatgcgtaccttttgtccgcctcccattgggagcccgaaaacaaatatcagca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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