XB-CLONE-3915937
Clone Name: IMAGE:7092006
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Gene:
hsp90ab1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654573 [+]
taattaatcccccccccccccctcttcattcaccaattccccagcagtgttcggccgcggagatccttaagcagccaagatgcctgaagtcgctcacaat
ggagaggaggaggtggagacatttgccttccaggcggagattgcgcagctcatgtccctcatcatcaacaccttctactccaacaaagaaatcttcctgc
gagagttgatctccaacgcctccgacgccctggataagatccgatacgagagtctgaccgatccctccaagctggacagcggcaaagacctgaagatcga
catcattcccaaccgccaggagcgcacactgactgtcatcgacaccgggattggcatgaccaaggccgacctcatcaacaacctgggaaccatcgccaag
tccggcaccaaggctttcatggaggccctgcaggctggtgctgacatctctatgattggtcagttcggtgtgggtttctattctgcgtacttggtggcag
agaaggtcttggtcatcaccaaacacaacgatgatgaacagtacgcgtgggaatcctccgccggcggctctttcaccgtgaagactaaccatggtgagcc
cattggccgtggtaccaagtcatcctctacctgaaggagatcagnacgagtacctggg
3' EST: CN324482 [+]tttttttttttttttcgctgtaaaaagatctttattttgcgcaacatttctcaactttacaacaacaaaaaataaaataaagttccgtattgaggctctc
gatagagagatgtgtaggatgggggtggggtctcagcattcaagacatcctgggggcgggggcacgagattccagtcagagagacgggggctaccactca
gcagatttgggggtgctacagatggaggtagctttgggcagaagtacctcacccccccagactggcctggcgtttctatttgcaaacaccagcacattca
atcgccccccccatgcaagtgaatgggagaaggcagcaggggggtctctaaagcttcccagtaagtaccccccgtgcctcattaacctatcaggcacaga
gaggggcacgcccattacaatagtggcagagggtaggattagaggggggcctgtaaggtgcattgagagagtcgaacagaccccccaagcagctggctaa
cagcgggccactaactcagggctaccacaagccaagctagctgccatactgctacaataacttacataactaactgctcagactggaacacaacaagggg
ggtgcagaggatagggttaaagtcagcagccctcagtcttgaagctccccccaatatattctataaggagggaagtccctccccagtattctgtgatatt
taaagcccaagggaaaagtccccccccctccgggtttaagaaagtgcacttacgcgggggggaaggggggcgggacgaaccgggcagcaaaccgatttat
ccaactttccccctggccaaaaaacttcctcccccccccccctctggggggggggaatctccttctgcccccccg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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