XB-CLONE-3915935
Clone Name: IMAGE:7091275
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Gene:
snrpc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653924
[+]
taattaatcccccccccccccgcagaagagacgtacgcgccattaggatcatgccgaagttttattgcgactactgcgatacgtatctcacccatgattc
gccttcagtccgtaagactcactgtagtgggaggaagcacaaggagaatgtaaaagattattatcagaagtggatggaagagcaggcacagagcctaatt
gacaaaaccactgctgcatttcaacaaggaaagatacccccaacaccttttgcggctccgccagctggaagtgccatgatcccaccacctccaagcatgg
gtgggcctcctcgacctggaatgatgcctgctccccccatggctggccctcctatgatgccgatgatgggaccacctcctccaggaatgatgccagtggg
ccatggtcctggtatgagaccacccatgggagctcacatgcctatgatgcctggcccacccatgatgcgtccaccgacacgccctatgatgctacagtcc
cggcctggcatggcacgccctgaccgatagaatttttacagttatttgtagctgtgctaaactttgtagtatggatgcttattgcagttcatgcaaatca
agaaaatcacagcactcgtacaagtgtgcctgctctgtattgccaaaaattggtgcaaacccatcgatgttaatctctaaataatttgctctgatttaaa
gggtatcaacat
3' EST: CN324498
[+]tttttttttttttttcttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttcccccccc
cccccccccccaaaaaaccttttagggaaacctttttcccccccgggggcaaaaaaaaaaaaaaaaccttttaaaaaaggggaaaaaccccccccccccc
cccccccccacccgggggggggggggaaaaaaaatttttttccccccccccccccaaaaattggggaaaaaaaagggggggaaaagggaaaaacgggaaa
aaggtcttttcccccccccaaaaaaaaacaaaaaaaaaaaaaacccccccccaaaaaaccttttcccgggggggggggaaaaaatccctaaaaaaaaaaa
aaacccgggggttttggggggaaaaaaaattttcctttccccgggggaaaaaaccccaaaaaaaaaaaatttcccccctgggggggcccgggggaaacca
agggggcccggggaaaaccggcccccccttccccaaaaaaaatttaaaacaagccccccccccccttccccccccccccggggggggggaaacgggagaa
aaaaaacccggttggacccccctaaaaccccaaaaaaattaataaaaaaaaaaaccccggggggggtcccccccttttggggaaaaaacagcggcccccc
tgcgcggggggtggtatttttgggttgccaaaaacgccaaaacccccccccccaaaaagttcacccccccccaaaaacaggaaaaaaattcttggggggg
ggggggcccccccccccgcgggggggggggacccccaggggggggggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: