XB-CLONE-3915817
Clone Name: IMAGE:7091215
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Gene:
ocm4.3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653907 [+]
taattaatcccccccccccccccacacatctgtcacaggaagactgcttgtatctcttcactccagctacagaggacttcggactgaccatggcattcgg
tggtatcctgagtgatgctgacatctctgctgctctgaagaactgccaagctcctgactccttcaacttcaaaactttctttacccagtctggcctgagc
agcaagtccgacaatgatgtgaggaacgtctttgccatcattgaccaggacaagagcggcttcattgaggaagatgaactgaaactgttcctccagaact
tcagcgcaggtgccagggctttaaccgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgaattcca
gtctctggtgaaaaactaaactttttgttaaaatctgaatctgtgaaacatcacaaaagatgaatgcgggttttctgcgttaatctgatccaagtgaaac
ctggtttggcggaacacagacgtctcatcaatatttctccccctcagaaaagtggtgtcatcatctggtggcgggattcatttatttattcacaaaaaag
cagttgtttttcttcacattttgtgacccagaaatggtacccttgccacagtttatcaacagccccttatgaaccaacatatttatctcattttatctat
aacatgtccatgtta
3' EST: CN325292 [+]tttttttttttttttctttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
ttggccccccaaaaaattttaaaaaaaaaaattttttttccccccccccccccccaaaaaaaaaggaaaaaaaaatcccccttttggggtttccaaaacc
ccccccgggggaaaaaggggggaggggtttttaaaaaaaaaaaaaaaaaaaattggggggttccaaggggggggggttaaaaaaagggggggggggggaa
ccctttgggggggccaaaaaggggaaaaaaaaaaccccccttttttgggaaaaaaaaaaaaaccccccccccccaaaaaaaccccccccttttggggggg
ggaaaaaaaaaaaaaaaacccggggcccccccccacccggtttccctggggaaaaaaaaaacaaaaaaccccccccccccttttggggggggtcccccaa
aaaaaaattaaaaaaaaaattttttttttccccaaaaaggggggattcccccccccctttttccccccccccccaaaacccccccccttcaagggggggg
ggggcccccccccgggaaaaccccgcccccccccccccaaatgtgggggggaaaatatttttcctccctccccccaaggacccccgcccgcccggggcaa
ggggggagaaaaacccccccccccccttgcgggcccgccggccccgcgcccaaggggggaaaaaaaaattttaaaagagagggggggggggccggggcgc
tcctccccaaacccacaaaagaccccccccccccaaaaccccccccaccggcggggcgcccccccacccccccccggcggggggggaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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