XB-CLONE-3915813
Clone Name: IMAGE:7091383
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Gene:
gan
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653620 [+]
taattaatccccccccccccctctgacactttttggatttcccagacacagactgtaagattgttctctccaggactttccctctgccgctgtgctgtgg
ctctatagctagctatgtacctctcacctttagtgtgacatagttctgtctccttcacttacctgtctttgtacagaaatacacggtcacctgatctaga
tgtctgaatctgagccccagtgcacaatgaccgaggtgtgtgacccccagcatgcggctaagctgctccgggctcttggttcattcagaggggagtccgg
cttctgcgacgcactactggttctagaggggcaggagatccctgtacagagaaacatactggcagcagccagtccgtacataagagcaaagctcaactac
aaccccccaaaaaatgatgggtccatttatcggattgaattggaaggcatatctgtggacgttatgaaagaaatcctggactatatcttcagtgggcaga
tcaggctaagcgaagagaccatccaggatgtggtgcaagcagcagatctcttacttcttactgacctcaaaactttgtgctgtgagtttctcgagggctg
catcactgctgagaactgcatcggcatccgtgactttgcactgcattactgcctcaacatgtgcattatgtggctaccgatttctggagaccacttcaga
atgtcagcg
3' EST: CN325294 [+]tttttttttttttttttttttttttttttttttttttttaatttaggctggaaagtttctttattaatgcaaagacaatccaaggtgtcacaaacaatgt
caaacacacaggcaagtactgtttatggcaacttgctgcttatttaaaaaaggaatgtctacttttggacctacagctgattctgccaaatagcccaaga
tgtagcaggaaaaaatctgtgaggctacccatcatacaaaaaataacaatgccttgccattacctcaagcattggaggtagtggactccaagcattgtca
tcaggatcatactgttctccagtgtccagggtttcgttgtcttcattttgacctccaagtacaaacaaaaatccctctgcaaaaagaaccccatggttta
tccttggtgtgcttagtggtgctagttcaatccatagctgccgatttggatcataaagagggcacatacagcgcactgaagccagtggtttacgggatgt
tcgctcttcaccaccaactgtaactatgcactctgagtagcccctcggtttaaaggaagccaacactgcctctccatgctgaggctggctaagcgggatg
ttgctgcactctctgaccatctctcggaccaagggctcaataagcattttctctcgcagatagctggagtccaggccagaaacccataaagcagagctta
catctttcatgttgactttttcttgctttcaaatcatgggaaacccatcgaaatacagcttccaagaaaatattttcctttccctacttttgagtttttt
ccaaagaacaaaactttccttttgctttttgtttggaacggaaccccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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