XB-CLONE-3915775
Clone Name: IMAGE:7092092
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Gene:
atp1b1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654331
[+]
ttaattaatcccccccccccccccccattcnnaaagtaaaatattatnaaatgggtggatttgcaggatttccactgaactactatccatactatggaaa
actgttgcagcctgaatatctccagcctcttattgcagtgcaatttaccaatttaacatttaatacagaaatccgtatagagtgcaaggcctatggtgaa
aatatagactatcatgataaagaccgctttcagggacgttatgatatcaaatttgatataaagagtagctgatctcaagcacgacctgcataaatctccc
ccccaaaaaacttctagtcttgaacaaactgtcatgtatgggacctacactaaatatatatgctttacactagcttcccgcttctactaggttagaatgt
aaaaatcaaggtgtagcaatagaaagaagtatttattctatggtaaatgatccaattcaatgacttgaaccttgggaaatgcccattttgctgtaaattg
ttattccatcatcaaaatgtagtaaaagcagtgtttctggccacaaactatagctgcttttcttttttttattttagtgtgtttgtacagtaagataagt
gcagattctacagtctcggccaagccatgttttgttttgtttcggctttgtgaccagtttgtatcccaggtgtaattatctgtaatggggataaaactgg
catgttt
3' EST: CN325523
[+]tttttttttttttttctaaaacaccaaactttgtttattgttttattgtctcatgtttaacttgactgaagttccaacaagagcacaggactcctggcaa
ttattacagatgtatgaacaatgtgtgcacagtagacatttaaatacatcctatgggatactgcattgccatgaacgttgttttaaaataaagatttatt
tttcaacaggttactttggtttgtttcatacattttctttttcttgaagaaaaataaaaattgtacaactgcataaatataaaattcttccaccatgaaa
atggttaaaacatttgtaaagacaaagcaccaaactgtgatgctttcagaaaaaacaaaaaaacaaaaacataaaataaaaatatttatatatatatata
actcaaaaaccattagccctatttctgacaaaaagtgacagtcggacccggggcttcacacaccttcataatgggcttaagcatttaaaggggaaagttt
ctataaaaagcattaaataaaaagcaccgcacaacccatcacctcttgcatttcagtgaaactaagaagagaaagacaaaaacagaagctcttttacaga
taaaacaaatcaatgccttatttaaaggacttgtggagtgctattaaatattattngttgggccaccactccgtttaaattaaaaaagaaaaacaaaaca
aaaaagaagcaataaaaattacatgtccgttttttcccccatacaaactatttatccccttgaaaataaaaacttgcctcacaaaacaaaaacaaaacaa
aaaaagggcttgggcccaaaactggtaaaaatctgcccccttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: