XB-CLONE-3915583
Clone Name: IMAGE:7091246
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Gene:
nup35
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653676
[+]
taattaatcccccccccccccgcttggattctatttcctggaagcatgatggccgccttctccatggagcctatgggtgctgaaccaatggcacttggct
caccaacatctccaaaacctagtgcaggtgcccagttcctgcctggctttctactgggggacataccaacacctgtaacaccacaacaacgtccatctat
aggagttatggaaatgcgatcacctttactttcaggtgtttttctgcattcaggtggctcacccccacaacctgtagttcctactcataaagacaagagt
ggagcaccgcctgttagaagcatatatgatgacattgcaagtccgggtcttggttctacacctctaaatccaaggaagacggccagcttttctgtgttgc
atactccactttctggagttataccgtcaagtccagcttcaagtgtcttcagtcctgcaaccattgggcatagcagaaaaactaccctatcccctgctca
aatggatcccttctatacacaaggcgatgccctgacttccgatgaccaattggatgatacttgggtgactgtatttgggttttcacaagcttctgcttcc
tatattttactaccagttgcacagtatgggaacatcataaaacatgtgatgtcaaacatggcaacggatgcatatcaatac
3' EST: CN326674
[+]tttttttttttttttcaatttaacaatttatttgaaaacaggctaaaacattaaaagacgatgatagaagaaatttgtgtgaaatgcaatgttacatttt
ccaaacgtaaaatcaatcatataaatggccttaaaacaataagacaactacacaggctgggcacatttgtttccattactgaagaccctatgtatgtaat
agtacactggcaaccagcctggaagcaagaagcagataaaaaaggatttggatataaagaaatggatgcgttcatggttgtaagccttagtgagagtttt
gacatgttccaaagaaagacataattataagtaaagtatgatacaaagattttatatatctacatatttatacaggaagagcagtgtacatcattttttg
acgcatttatcttcataaatgcatttcattaaggcaagagagttccatattcgcctgcaccagtggcctgtcatttctggttatagaactaacccagaag
cttggtggcaggctgagcagaggggaaaatgctaccagccaaacatatactccattgcttttgatacaatactctcatcctttcgaggggcttgtttatc
agccacaacctgatagtcggcactagctggtgtttttgtagtagcaccaagggggctcatggatgcggctcttggagtacccaacaaacgtgtaggagtc
cctatgttctttactggtggtgtgaaaaaggaaaaaacaagagggggttgacacttttccctggggtttcctttcacccctgttcaaaggaagggttttt
ctcccattttttttgcctttccccccaaaaatcccttcccctcttttaccccccccccctttttttg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: