XB-CLONE-3915557
Clone Name: IMAGE:7091957
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Gene:
pcbp3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653799 [+]
taattaatccccccccccccccgagaccagcagaggcagcagcagaagccgcagctcccgccagcccctcccgccgtcgccattcctcgcccgcccggct
ctctccttccctccctccccggggccccttgcctccggaccgttccttcctccccattcctcccttgttttcctaccccctttccccattttcttaatcg
ctctcacttttcccgcccttccaccttacggcggtcacctcttgtcctttgtaactccgcagtcttgtctcatcctgtgaccaaagatttgactactttg
tacagcttctgacaatagccctgatccaacagaactagactcatcatggacaccggtgtgattgaagggggcctgaatgttacactcaccatcagattgc
ttatgcatgggaaggaagttggaagcattattggaaagaagggggaatctgttaaaaagatgcgtgaagagagcggtgcccggataaacatctcaaaagg
gaattgtccagaaaggataattaccctggctggtcctacgaacgtcatctttaagcctttctctatgataattgaaaaacgggagaggatatctgcagct
ccatgacaaaagcactgctactagcaaactccggttactttgcgctgggggtcccgcaagtcaggggggatccccattgggaagggggtgcaaattaagg
aattagaaaaagtacggaccccgggaac
3' EST: CN326918 [+]ttttttttttttttttttttgttgttaaaccgatggctggttttatttttaattaaaacaatactcacagtcaatgcttacatttccaattggtgaatga
agctaataacagctatcactactggcaggttacaaagaaaagggcaagcaggaaactagtctttccaatcagtaataaacgtccaatttttgggtgaagc
ttatcgctagcatttaaggaattacagtggatgcaacaaacacacatatgattaaatctggacagaaattaaatgtgacggctcttatataagacgatgg
gaatttcagtagggagagaaccgctgcttttccgatttcgctttagagtagagctgggcggcagaggcaggagcagcagaggaagatgaggtagaggagg
aggcggtggacacgatattaagagccaggagcgggacgggtttcatgtcaagcagattggagacacaaggggcagtcgagggagtgggaaggctggaagg
tgtatccgagtaggaggaagaggatgtggaggctgaggagggttcacaagccccggctgtggaggtgatcaccagagaggaaggggcggcggaggaaagg
gtggagggttgagagatgctgagaaggtcaggggtcgtggcactctgggaaaaggagggtttagcggtttctagactgacattaataaagtactgggcca
aactaatgctggctgtggatcccgtttatggtcacctggcgatcatttgaaccctccccagggtttacctatttttattctgaacccccaaaactcggaa
caaaattcctttttatttttagcctcccctttcctccaaatgaaaaacaaccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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