XB-CLONE-3915421
Clone Name: IMAGE:7091947
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Gene:
oip5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654520 [+]
taattaatccccccccccccctggttattgtgtagtgctgcggcaatgcagtctgtttgcggggaaagcacggtggacttggagcagacaaacccagagg
tgtcatgtcggaagttaaatggcagttcgggcgggcctgaggcgtgtgtggtattcctgtgtaagaactgctacacggtgctgggggactcgctcagtgt
gtgtggggaggagaggagcttggctgccatcgcctgcctgcgagtcacagaagatttgaatgtaggagaatcagtactttttgtggtgaatgggaacctg
aaaggctgtgcctattaccctttactgtgtcgctcctgtgggaccaatattggcttcaacctttactctgcgcctagggcttatgcctacctgagaggct
tattctgtctcctcaaagaacatattctctgctataaactaaaaatccagcgctttggtcccaggaaatgaaatgcaatttgagttttccaagcctagag
gaggatgtggctaaactgaagatgcaactggtgcagctgcatgttaaaatggaactgataccaaactactgttacagcaggagaaaaaactacagcctgt
tcttgacatgcacaactactaactcaagccttgctactgattttgggaggagctgcccaattttctcatttggtgacctaatttgtacaggtttttc
3' EST: CN327752 [+]tttttttttttttttgttgtccagatacttatttaattttaacgcagaaaatgtatgcagaccactttgtacaaagcattaaaccaccgattctattttg
cttcaatatagattgtatacatacacacatactgaaggtagggaaagggaaccttaaagattatagaaggctagaaatgcacactatgtgctgggcttct
gtaccagcccaaagcaacaactgccctcaaggctggttagtgattcagattcatggatgcttatgggtgcttgtgacaaatgaacctcattttgtgcttg
atttggaatgacccctaagcttagcttctcaacagctgcacagagcaagtgcaattcaagatggtgactccctgtgcaaaactttgaaggcctaaattac
tgcaggtatagaggttctgaaacttaagactggtgctgtaagttcagtatataaaatatgggccttacagtcatgttccctgttacccaactggtatttt
ggattagtgtggctcaattttattgggttttcttttaattaaggctcaggagaacatactcctgttcaaatctcacaattctcaacacaaaaaagagcta
atccattccatgtcagacatcctgtcgaagagtactaaacctagtacataaatttgggtcagccagatggagcaaagctgagcagacttntcccaaaatg
cagttagcatggcttgagggttagtttgtgtggcatgtcatgaaacaggctgtagtgtttttctccttgctgtagcagttgttttggaatatagtttcaa
ttttaactggcaacttgccccatttgccatctttcagtttctgccca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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