XB-CLONE-3915393
Clone Name: IMAGE:7091023
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Gene:
ran
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654063 [+]
taattaatccccccccccccggctgctttacagacgcttctggaaggtgagattctaacccgtgggagatataggctgggccctgccgcataatccgccc
gacatctcggccatccctattcgcaaagcgtcccgaagctcttgtagcactcgctggttaatcgtgcggccgggcacggtagatttgagagggattctag
tcggctgacaaacataccatggcagcccaaggagaacctcaagtgcagtttaagcttgttctggttggggatggtggtaccggtaaaacaacctttgtga
aacgtcacttgactggtgaatttgagaagaaatatgtagccacacttggtgttgaagttcatcccctggttttccacactaacagagggccaatcaagtt
taacgtgtgggatacagctggtcaagaaaagtttggtggtctcagagatggatattatatccaggcccagtgtgccattatcatgtttgatgttacatca
agagtcacatacaagaatgtaccaaactggcatagagatcttgtaagagtgtgtgaaaacatcccaatagtcttgtgtggtaataaagtggacataaagg
acagaaaagttaaagccaaatcaattgtcttccataggaagaagaatcttcagtactatgatatttctgctaaaagtaactacatttttgagaagccctt
cnttnngctgcagga
3' EST: CN327973 [+]ttacaacctctggtggggcaagggctggcatggctacaaactcaagattggggtcacctataagtttccttgcaagccaaaggaagggcttctcaaaatt
gtagttacttttagcagaaatatcatagtactgaagattcttcttcctatggaagacaattgatttggctttaacttttctgtcctttatgtccacttta
ttaccacacaagactattgggatgttttcacacactcttacaagatctctatgccagtttggtacattcttgtatgtgactcttgatgtaacatcaaaca
tgataatggcacactgggcctggatataatatccatctctgagaccaccaaacttttcttgaccagctgtatcccacacgttaaacttgattggccctct
gttagtgtggaaaaccaggggatgaacttcaacaccaagtgtggctacatatttcttctcaaattcaccagtcaagtgacgtttcacaaaggttgtttta
ccggtaccaccatccccaaccagaacaagcttaaactgcacttgaggttctccttgggctgccatggtatgtttgtcagccgactagaatccctctcaaa
tctaccgtgcccggccgcacgattaaccagcgagtgctacaagagcttcgggacgctttgcgaatagggatggccgagatgtcgggcggattatgcggca
ggncccagctatatctcccacgggttagatctcacttcagacgctggtgaaacagcgggggggggggggggggggattaatttacctcgagtgcaaaaaa
aaaaaaataatcttgttatccgggcccgggaataaaaccaaggttgcagttgtggacctggga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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