XB-CLONE-3915349
Clone Name: IMAGE:7091374
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Gene:
serbp1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653744 [+]
taattaatccccccccccccctttgttctttgtttttttttcccccaactttaaatcttttttttaacatgctagaaattacccatgtattggcaacttg
aactggtcattttcacatctgaccttgccctttgtctagctaaagtcccaatttctatatatctattttcaacttgcaaaggtcattttacttttttttc
tagattgtgtaagaatacacaaaatttagacattttaaagtacatgaatgtgcttgtcaattttgatcctgtgggtaaatggtgttaatggactgcaact
gtagagaattggtaatgtaacgtatcccagtgtgtatgcttatttagcataggatttaattaacatcgccctgagagttatggtaatttccataaactct
ctaatggaatatgaattttcattttgaattggacatactgagtagcacaaacatgtatccagtgtatgatagcaaaaccaataggaaagtcaaacctgaa
aatgccttcatgcaacaaacatcagaagtcaaagaagttactgtgttgcttgttccatttgctgcaacccgtgtgtgaggagaattgctttataaaagag
cagcattactgaagtgtttgtcacactggtcccttgcattgtgaagagctttttcatttngctgggaagtagaca
3' EST: CN328209 [+]tttttttttttttttgccatattacccttcattaggctagagcatgaaaattaacctttaattcaataagcatccaccagacagatttgttcacagtttc
agttcacacagaaatgggcttcagataaatgtacaactatgatgggtaatgaaggggaaaggcacattaattcttcagctagtgactggaaatcccagat
tgtcatttaccagctagcaacagatgctcaaaggttcattcagttcttaaatgaagctgcagtgcctttatttatagccaggagacctggttcgacaaag
tgccaattctcagatttgatacaagttcactttaactgaaaatgatgatattcctcaggcaaaggaacaagccaaggttcataaatgaatagattagctt
gaatggacagtacaagactgttgtacaaaagtaaagaagcctatagcaaggatgtttcagggccacactagcatgttactctgcatgtgtaagaggctct
tagataacttaagggatctgtacataacttaatctcagctgcttaaacatgtttccattttgtgaaggcttatttaaattgataggttaatgcattgaag
tgttatctttaaaaactccactggtttgcagtctcatagtaaattatctctctagaaaataatctcaaaatgggcaggctgttgaataaaaataccactg
accccagcccgcggccagaaacccgcctaccccctgtaaaagtaccacagtataaagccctccgggccactgaaacaatgaagctggcctttttgggacc
tcaaaaaaattcaaatacctttggttgccttggtcctggttaaaaacttttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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