XB-CLONE-3915309
Clone Name: IMAGE:7092062
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Gene:
gak
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654588
[+]
taattaatccccccccccccccccgggaagcgcgacggagaacgtaggagagagagccgggtcctgtcagcagctgtgacatccagcctttaaatacacc
ggaggggtactggcccttttctctccccgggatatgtcgctgcttcagtcagctttggattttctagctggtccggggtccctcggaggtgcaggccggg
accaacatgacttcgtgggacagacggtggagatgggagatatgaagatgcgggtcaagcgagttattgctgagggtggttttgcatttgtgtacgaagc
ccaagatgtaggaagtggaaaagactatgcattaaagagattgctgtccaacgaagaagagaagaacaagtccattattcaagaaatctgtttcatgaaa
aaattatctggccatcccaacatagttcagttctgttcagcggcatccattggaaaagaagaatccgacactggccaaggagaatttctcctccttacag
aactgtgtagaggacaacttgtggaattcttaaataaggctgaatgtaaaggtccactgtcctgtgatacagttctgaagattttttacaaacatgtcgg
gcagttcagcatatgcataacaaaaaccaccaataatcctagggacctcaagtagaaaactacttgttagaatca
3' EST: CN328445
[+]tttttttttttttttggatgtcacaagcatctagatcatactttgcaaactttactgttgtagcatttcgtggcacaaaccctgtatggaactgaatctg
gaacattttcattgaggtcatctttgcttgcaatctgccgccaagtgtagaccgtgcatgatagataacaatcaggacatccccttgcacagtaacattt
aaaggaatttcagctttgccatcttcatttctaaagtctctcattttatcatactcttgagatgttgttgctactctctcatcccccacataaacttcac
aaaatggtctgcaaccattgcgttgtttgctgaacagaggtactggtgtcatggttatagccctaataaagataggcttgctgtggggaatgattggctc
ctctgccatcatgtcacacatgtactctatataccttttgtgagaaggccatatacctggcgggcaccgtttcatgctgaacatataaactgcagcttca
gcagtggtaaacagacggcagaaacaaataaaggaacacaacccaactgctgatgcggttcttcccttctgtgcattgaacaatgcaaacgttcttccga
tctggttcaaccaaaaatgcatatttttgaaatgctgaaaaggttccaaaaattggaaccagtctttcaccccccccacattctgaaaccctggtaagaa
acctttaaagggcttatttttcttaacaaaggttataaaagccataagttccggggaggttttgagtcccaaaaaatgggaacatccccaaaattttttt
ttatagggcaaattcccccctttctcccgggaaaaaaaaaaataacaccaatctcctttggtgtttatgtatatataaac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: