XB-CLONE-3915203
Clone Name: IMAGE:7092087
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Gene:
actc1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654548 [+]
taattaatcccccccccccccgacccagccaatttaatcacagcaaccagctctcctgtgctgaccagaacctaacctgccactatgtgtgacgacgagg
agactaccgccttggtgtgcgacaatggctccgggctggtgaaggctgggttcgctggggatgatgccccacgtgctgtgttcccctccatcgttggtcg
cccacgtcaccagggtgtcatggtgggtatgggtcagaaagactcctacgtaggtgatgaagctcagagcaagagaggtatcctgaccctgaagtacccc
attgaacacggtatcatcaccaactgggatgatatggagaagatctggcaccacaccttctacaatgagctgcgtgtggcccctgaggagcaccccaccc
tgctcactgaggccccactgaaccccaaggccaaccgtgaaaagatgacacagatcatgtttgagaccttcaacgtcccagccatgtatgttgccatcca
ggctgtgctgtccctgtacgcttctggtcgtaccacaggtatcgttcttgactccggtgatggtgtcacccacaatgttcccatctatgaagggtatgcc
ctgcccctgccatcatggcgtctggacctggtgggaaagaaccccaagattacctcaggaaatcctgaatgacgtggctaacttttgtcacccagttgaa
gggaaattgccgggaatcag
3' EST: CN329153 [+]tttttttttttttttcttttttttttttttttttttttttttttttttaacgtaaccgcttgtgttttatttaaaaagtaataaatatgatggagtgtaa
acaaactgtacagtgactgtccaggtcttgttttcttcgactttgaggtactttgtatgtgttgcttggaggagtgtgcgtaatgtttaaaagcacttcc
tgtgcacaatggaggggcctgcctcatcgtattcctgtttgctgatccacatctgttggaagggggacagggaggccaagatggagccaccgatccaaac
tgagtatttaccctcagggggggcaatgatcttgatcttcatggtgctgggagccaaggcagtgatttccttctgcattctgtcagcaataccagggtac
atgggggtaccaccaaaaaggacattgttggcgtacaggtccttgcggatatcaatgtcccatttcatgatgctgttgtaggtagtttcatggataccag
caaattccataccaatgaaggaaggctggaagagggtctcagggcagcggaaacgttcatttccaatggtgatgacctgaccgtcgggaagctcatagct
cttctccagggaggaggaagaggcagcggtggccatttcattctcaaagtccaaagccacatagcacagctttttcctgatgtcacggacaaattcaccg
ttcagctgtggtgacaaaagaatagccccgttcagtcagattttcatgaggtaatctgtggaagtctccacccgcccgggtcaacacgcttgaatggcag
gggggccggggcaaaaccctctcataaatgggggaaaattgtgggggggaaacccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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