XB-CLONE-3915051
Clone Name: IMAGE:7092030
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654315 [+]
tagttaatccccccccccccccgatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagaga
aaggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagag
acggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatc
tggccccatcaacttcaccgtcttcctgaccatgttcggcgagaagctcaagggtgctgatcccgaagacgttatcactggggcattcaaggtgcttgat
cctgaggccaaaggatccatcaagaagcagttccttgaggaactccttaccacacagtgtgacagattcaccccagaggagatcaagaacatgtggacag
cgttcccccctgatgttgctggcaacgtagactacaagaacatctgctatgtcatcacccacggagaggacaaagaccaggaataaacaggcccagtctt
gccctcctgatggagggggtgcgccatcacgcccagcaccaaccttgctgtatatgcgtcttatttttaaactcatccctcagaaaacaactgtatttat
tcttctagggtgg
3' EST: CN330086 [+]tttttttttttttttgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggaggg
taaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgtct
ctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcctgt
ttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgttc
ttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcaggatcaagcaccttga
atgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagacggtgaagttgatggggccagatgcctctttgac
catctcttccaactcttcattcttcacgttcaggcggcccatagctgcgaatgtgtccctcaggtcttccttgtcaatgatgccgtctctgttctgatca
atcactgtgaaagcctccttgaactcttggatctgggtttggtcaaacatggacaacacatttggaagattcaccttctgctgtcctttctcttggccct
tctttgctggttgccctggtcctccggttgaaccttggtgcctgg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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