XB-CLONE-3915033
Clone Name: IMAGE:7091265
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Gene:
rps6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653920 [+]
taattaatcccccccccccccgctctctttccggcgggggatctaagacagactggttgttggccatgaagcttaacatctccttcccagccactggctg
ccaaaagctcatcgaagtggaggatgagcgcaagctgcgtaccttctatgagaagcgcatggctacagaggttgctgcagatcccttgggtgatgagtgg
aagggatatgtcgttcgcatcagcggtggaaatgataagcaaggctttcccatgaaacagggagtgctaactcatggccgtgttcgtcttctgttgagca
agggtcattcctgttatcgccccaggaggactggtgaacgcaagcgcaagtctgttcgtgggtgtattgtggatgctaacctgagtgtcctgaacttggt
tattgttaggaaaggcgagaaggatattcctggacttacagacaacactgttcctcgtcgcctgggtcccaaaagagccagcagaatccgcaaactgttc
aacttgtcaaaagaagatgatgtgcgtcaatatgtagtgaggaagcctctggctaaggaggggaagaagcccaggaccaaggcccctaaaatccagcgtc
tagtgaccccgagagttctgcagcacaagcgcagacgtattgcttttgagaagcagcgcactcagaagaataanngagaggcatcagagtatgctaactt
ctgggctagagaacaaggaagccagga
3' EST: CN330173 [+]tttttttttttttttaagtttacaacaaaatgtatttatttttacatgatggagttttatttctgactcgattcagatttggatgtggaggctctcaaag
aagacagtctgcgcctcttggcaatttgctcctggcgtttttccttggcttcctttgttctcttagccagaagtttagcatactctgatgcctcttcctt
attcttctgagtgcgctgcttcttcaaagcaatacgtctgcgcttgtgctgcagaactctcggggtcactagacgctggattttaggggccttggtcctg
ggcttcttcccctccttagccagaggcttcctcactacatattgacgcacatcatcttcttttgacaagttgaacagtttgcggattctgctggctcttt
tgggacccaggcgacgaggaacagtgttgtctgtaagtccaggaatatccttctcgcctttcctaacaataaccaagttcaggacactcaggttagcatc
cacaatacacccacgaacagacttgcgcttgcgttcaccagtcctcctggggcgataacaggaatgacccttgctcaacagaagacgaacacggccatga
gttagcactccctgtttcatgggaaagccttgcttatcatttccaccgctgatgcgaacgacatatcccttccactcatcacccaagggatctgcagnca
cctctgtagccatgcgcttctcatagaaggtacgcagcttgcgctcatccntccacttcgatgagctttttggcagcagtgggctggggaagagatgtaa
gcttcatgccaacacccatctgctaatcgcgagcgaagagaggggggggggggggggggggggggggggggggaggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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