XB-CLONE-3914977
Clone Name: IMAGE:7091199
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Gene:
myl12b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653778
[+]
taattaatcccccccccccccggtctgtgtgtaagggtctgccagcaacctcggattttctattctatcgccggtcgaataacccaccgccaagatgtcc
agcaaaagagcaaagaccaagaccaccaaaaagcgcccccagcgcgcaacttctaatgtatttgccatgtttgaccagtcccaaatccaggagtttaagg
aggcattcaacatgattgatcagaaccgtgatggctttattgataaagaagatctgcatgacatgcttgcctctcttggaaaaaaccctacagacgagta
tttagatgccatgatgaatgaagcacctggccctattaacttcaccatgttccttaccatgtttggggagaagctgaacggaaccgatcctgaggatgtt
attaggaatgcctttgcatgctttgatgaagaaggaataggttctatccaggaggatactctgagggaattgctgacaacaatgggtgaccgatttacag
atgaagaagtagatgagctcttcagagaggccccaatcgacaagaagggcaatttcaattacaatgaattcacccgcatcttgaaacatggagctaaaga
taaggatgacttagctggaagtgcccagtggtgtaagaacaactgcactgctctatatcatcctcacgttctcttcctgaagaactcttgcatgcattag
ctctagatctttctttctgtgtattatg
3' EST: CN330526
[+]tttttttttttttttagaaaaaaacccttttatttactttataaaaatgccgatattgatatgaaaatgtgttctctacatttagctcttaatcatgcca
gcactgttagccaatagctgtcctgtatgtggcttgctcattgctagcgagagtgcagcaagaacccttggcaggagatgaagaatacatgtgcagtgta
cagagctagaagccgggggcgcttgtctttaggagaagcaatgcgcagggctctagcggatataggaacagagaagaagcttgttgacacgttggaagtt
ttagctgggatatgccacaagcagcttttattgagaagagtaaatgctacaactgtcagataaacaagtctataggagccaactgcatagctaaccccag
tactaaacagggcctatttttaacaccaacatatatatatattcacataggaatatcttatttccactttgccaggccattcggagaaaagttttaactg
acagttatcttaattccaaattttactacaaaattgcaaaaaatccagctcaagtcccaagaaaaaacctgctgtgtaacgcgtgttacattgcactttt
ttttgttttccagtttggttacatatgtgcccatttgccaaaagggcctggttcagatccataaatacacagaaaagaaagatctagagctaaatgcatg
catgagttctatcagggagaggaacgtgaggatgaatagaagcagtgcagttgttcttacaccactggggcacttccagcttagtcatccttatccttta
gctcatgtttttcagaagggcggggggaaattcatttgtaattta
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: