XB-CLONE-3914973
Clone Name: IMAGE:7091910
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Gene:
kif18a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653867 [+]
taattaatcccccccccccccattgagcccggaagtggaggaactaggcgagcgggagatttgaatggagtccgctgcgaatgacagacactgcaatcaa
tgtgcggaaaggcaggatgtgagaaaccctgaagaggagagtggcacagaatctgggttatccagggacttcaagcaacattcccatttctgatttcaga
cacactgtttaagcaattcctagactagcactggataaatctgttctttgaagatggcagcctcccaagaggatgtctgcagtcatgtaagggtggtggt
ccgagttcgacccgagaacgaaaaggaaaagcaagggaactttagcagagttgttcaagtggtggacaatcatatcttggtgttcgatccaaaagtagaa
gaggttggctttttccatgggaggagcgcagccaatcgagacataaccaaaagaaagaacaaggaccttaaatttgtgtttgattgtgtcttcgatgaca
gttctactcaactggaagtttttgaacaaacaacaaagattgttttggaaggagttctcaatggatacaactgcacagttctggcttatggagcaacggg
tgctggcaagactcacactatgctggggtcaccgggggagcctggagtcatgtatctaacaatgatgga
3' EST: CN330543 [+]tttttttttttttttggtagtacgttttcagttcgttttctttgaaacgcatttccaggttgaggtactctgctcgtatttcttctcggttggtaaacaa
acatcggaaaatttcctgaaatctcttaatttcagcctgatcttgacctggaggaagcaaaacattctgcttcggtttcccaggagcaattgctttctga
tcttcatatgcctttaacttctcctttaatgccgcaatctctttcttttgttgctcgcatatcttcacatattgactaatgtgactgtccaggctgacta
cgttgctcttcactgctgattttatgtccttggctcggttcgcgtacttgagcgtattgtaagtgtcgtcgtaagacagcgaagaaggactaacagcggc
aatcataatagtgcggcagttccctccaagaaaatctttcaaaagacgtgtaagtttactgttccggtaaggaatatgttgtttcttgctctttggatct
gctagagcattgatgacgttccccagagccaacagtgagcgattgatgtttgttccctcctcgtaaacgttcacccttggcgttcgttgcacttggcccg
ttcagaaacagccagatcaatgaggcacattttggcaattcgaactttctggattgatacttgccgtcttgtcctgctgtcttaagtaaaatctaaaaac
aacaatgtgaccttgatgacaatcatttatgtaagtagggggctgggtttatttttgtttccctaatccaacactctgaaggatttccccccgggggact
tggggcgaatgtataagtgtaaacccttgcaaaaaaacattcccttttggggggcccccccccctccaagac
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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