XB-CLONE-3914953
Clone Name: IMAGE:7091121
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Gene:
rpp14
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653885 [+]
taattaatcccccccccccccccccggtgcatggatcccatgtgagcacgggaggatgagagagcaacccaaactagccgggcccacgagctatgagcga
atagtgctgaagaacccgtccgagcaccattacatgaaggttcagcttgaatttaatggagaaggggcccagctcagtgccacaattcaagctactggta
gtctctgccctgaagaacctgcacggagaggtgggatcgtctcttcctttggatctgttgagttttgatgaaaagacactgtgtgcagtactgagggtct
gtagcaggggcctagtgaagttgtggagctcattaacactgctggggcattataagaaccatgaatgctccattcgagtgatacagacctctccattcct
actggcgctggctggtaacagcagggaactggatctggaataacgccctcccctgggcctcattataaaggaagatgcctctgattccagtggtttggaa
agctgctatttatgtgcccactaggtcaccgcacatcacccgatggtttcatgtaacgtctcagcggcaatatagtgtaaaggtgggagactatgctgta
ctttcccgaaccttcaccaaaatgatattcgagtattctctgaactcactggggacacaancccctacactcggatgagctttcgctagggacgcgatcg
gaagactgttgtgctgagtctaatcacgg
3' EST: CN330643 [+]tttttttttttttttctttttttttttttttttttttataggtgaataaagggagttttatttagggatagtgaccctaacatatatacacaggctgttt
ataaagcacacatacagcatttataactttcaatgaatatttcacaaaatgggatttaacaggatttcttttcaaatactagcacattcactgtcccctc
catgacagtccggccactctcccttgccaggcaggacacagccacaatggccagtgatctctttagggccattacctgtgctgtagcagtcacctcctcc
ccaatatgtaagggtgcagggaagcggatatcctgggaaaggaggacacagcctggccctggcagtttagttcccaggacggcagataccaagccgttga
ttaggactccatgcacaacagtctttccgaatcgcgtgcccttagcgaaagcttcatccgagtgtagggggtttgtgtccccagtgagttcaaaaaatac
tcgaatatcattttgggtgaaggttcgggaaagtacagcatagtctcccacctttacactatattgccgctgagacgttacatgaaaccatcgggtgatg
tgcggtgacctagtgggcacataaatagcagctttccaaccactggaatcagaggcatcttccnttataatgaggcccaggggagggcgttattccagat
ccagttccctgctgttaccagccagcgcagtagggaatggagaagtctgtatcactcgatggagcattcatggttcttataatgccccaccagtggtaat
ggagctccccaacttcccctagggcttgcctacagaacccccctaattgcgcccaaagtgtcttttctc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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