XB-CLONE-3914945
Clone Name: IMAGE:7091335
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653609 [+]
taattaatccccccccccccccgatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagaga
aaggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacagag
acggcatcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcatc
tggccccatcaacttcaccgtcttcctgaccatgttcggcgagaagctcaagggtgctgatcccgaagacgttatcactggggcattcaaggtgcttgat
cctgaggccaaaggatccatcaagaagcagttccttgaggaactccttaccacacagtgtgacagattcaccccagaggagatcaagaacatgtggacag
cgttcccccctgatgttgctggcaacgtagactacaagaacatctgctatgtcatcacccacggagaggacaaagaccaggaataaacaggcccagtctt
gccctcctgatggagggggtgcgccatcacgcccagcccaaccttgctgtatatgcgtcttatttataaactcatccatcagaacacactgtaattattc
ttctaggttggt
3' EST: CN330727 [+]ttttttttttttttgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcgaaatgtgagggagggt
aaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaattacagttgtgtctc
tgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggcaagactgggcctgtt
tattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacgctgtccacatgttct
tgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcaggatcaagcaccttgaa
tgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagacggtgaagttgatggggccagatgcctctttgacc
atctcttccaactcttcattcttcacgttcaggcggcccatagctgcgaatgtgtccctcaggtcttccttgtcaatgatgccgtctctgttctgatcat
tcactgtggaagcctccctgactccctgggatctggggtttggtcaacatgaacaacaacattggaagattcacccttctgcctgcccttctccttgggc
cttctttgctggtggccctggtccttccggtttgaacctgggggcttgtcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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