XB-CLONE-3914939
Clone Name: IMAGE:7092041
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Gene:
angpt4.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654538 [+]
taattaatcccccccccccccatgtagagacagcaatttacattttggaggacaaagcattttcagagattaaatgggccttttctacttgagacaattc
ggatgtctgctgttcttgggtgtgcacgttcagtgggtttcatcagaggttttccccacagaaagccctctaggttatgactgttcgcatatctgggaaa
gaaacaatgaatctgtcagtgggatttacacaataaaacctctaggtgccagcacatcttttcaggttttctgtgaaatgaaagctgatgggggctggac
actcatacagagacatgacggacaagatggccttctcttcactagaacctgggctgaatacaaactaggatttggaaacatctctggagaacactggctg
ggactggaaaacatgcatatactgactaaccaggatagcagagcctcagaactgttcatcagccttgaggcctttgatgatagtgatggagccttcgcct
tgtacagctcattcatagttgctcctgaaagcaaattataccagctctctgtgggaaattattctggaaacgcaggagatgcttttagaactggaaacaa
caatcaagatggcaactttttaccaccaaagataaggcaatgacaagtgcaatcatgtaagattgtgacaccagttttcagttgcagccgtttcattctg
ttctggatggtggttac
3' EST: CN330771 [+]tttttttttttttttcttttgttcatttattcaagtgttcagtgaatacaataacgtataaaaagttaattaaaaaaacacaatagaatgaaaaaaacac
attaatagaaataaaaaagaaaacagatacaacaagcatctaataaactatttacagctgtacggcatattctatcagatgtttgctgcattctctgtct
tgtgtgagagaatgtttccttattagcacggatatatttaatgggcacctatgcccttgaacactataggtctctttacaaatatatcacataaaacaat
ccatatgtaaaacattgttttatataaataaagccttttcatggaaaatatatttttctagtagtgtgtaccactagataatcccatatagaaaatgcaa
ttgtaactactaccctgtgggcacacaaccaaaggcacttatacatgctaggatgtcagccaatgaatggacagagctgtgtcttttccacttgggctca
ttcctgttccagttagaacctacatatttcaatgagtgacactaagaacattacagaatgttggctcaagttatacaaggccaaatttgactgccaactt
gataaatatttaagatgtcccttgttatgatattgattatatgttggttcattatttattctgaaggtatagttttcatttaagtgactaaattaaaaaa
aacccacaaagatatctgaggtaatcaaaatactgncataatccttcaaatatcaaatatttagagttgcaggagggatccctgttgtgcattgtttaaa
ccattttcaaagaaacaaccccaaaaaagggatcaaaaaat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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