XB-CLONE-3914931
Clone Name: IMAGE:7091167
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Gene:
cldnd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653895 [+]
aaagactccctctccctactggaataaggggtgaatatggatggtctttctgcttagcttgtgtttgctcacccctacaggttatggctggagctctgtt
tctttgggcatctcgtgcaagccgtcgagagtactctctaatgaaggcttatcgtgttgcatgaaagtagttgtatttatgaaaacagtttgataggcac
ctgtaaatctagccttgttttatattttaaggctttttttttcttttagcttcctcattcttctagtgatgagcaggtaaagaaaaaaaaaaattaaaca
aacttgtcccttactctttatttacaaccatttctggcagtgtccatccattttttttccacaaattgtagatgactttcatgggtttctgtcacccact
tatcacttatttggtactttacataaatggttgattcacagtgcagctctggataggaagatataaaaatatttcttatgcatggtaaaagaccatattt
tcttgataagacttggtacaattaatactgacaatgcacttttagagccatcctttatattagtataggtaattgtagttaaaaggacattgttatccct
tgtggcaaaattgctcccaagcagcaaatgcacccctctgctaaaaactgggggcacccatggcagctgctgttgatgaacactgtcccatgtaactgtc
tttatctattaaaaaacaag
3' EST: CN330833 [+]gtccctgacccacaagttgaaatctccaactcctttcctcctcttcccggtacagcgcgaagcaccaacccctcttcctgttcagctctccttatctctc
tgactacagggtagtccaagaagagagccttctgtgcatgtgtgaccgagaagcagctttctggaaatgcacagagaagccagtgcacagcagcatcagc
agatttaaaagttaaacaggaagagggggcggggccccacgctgtactaggaagaagaggaagccggagacctcagcctataaatcataaaccagatcag
actgaatgcaaggactaaaggtccccatacacaggcagatccgctcgcttggcgatatcgggagaatccaggataatttgacaattgaattgtaacgacg
ggcataggaaaagtcggtctggggactgcatcaacgagccaatggggtccccgatccgactagaatttctaacctgctcgatcgagatctgcccaatttc
tgaccagatatcagtcgggtagacccatcggtagtgcccatacacgagccgattagctgcggaatcggtctaagggaccgatatcggcagctagaatcgg
cccgtgtatggggaccttaaggtatgttattcttagggttgtctagagtaattttaaagatagggaaaaaaagaggtttacttctttaaacagcagaatg
catattagtttaatcataccatagaatcatagattcataaatctagtttacattttttttagaaaaaatatacatacactggttttgctactccttccac
ttaattttatggtaattgaaggatttatttccggccggggaaaacacat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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