XB-CLONE-3914929
Clone Name: IMAGE:7091244
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Gene:
rps3a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653675 [+]
taattaatcccccccccccccgctttctctcccagctctgcaagatggcagtcggcaagaacgagaggctgactaaaggtggcaagaagggtgccaaaaa
gaagattgtggaccccttctccaaaaaagactggtatgatgtcaaggcaccagcaatgttcaatatccgcaaccttggaaagactcttgtcaccagaacg
caaggaacaaaaattgcctctgatgggctgaagggtcgcgtcattgaagtaagcttggctgatctgcaaaatgacgaagtggctttccgtaaattcaagc
ttatcacagaagacgttcagggcaagaactgcctgaccaacttccatggcatggatctcactcgtgataagatgtgctccatggtcaagaaatggcagac
catgattgaagcacatgttgatgtgaagaccacagatggctacttgctccgcctgttctgtgttggattcactaaaaagcgcaacaatcaaatcagaaag
acttcctatgctcaacaccagcaggtccgtcaaattcgcaagaagatgatggaaattatgacccgtgaagtgncaacaaacgaccttaaagaggttgtta
taaactgattccagacagcataggcaaagatattgaaaggcccctgcagtcattatcctctcacgatgtttatgtcgtaagg
3' EST: CN330834 [+]tttttttttttttttaaggtttattttacagtttttaaatcagaattctttaactcagaccgattcctgcactgggggttcatacccatcagcacgttcc
actttggcaccagtctcgtctcctgctggttttccagctcctccaccctcgccatgaagttccattagtttgcccagctcaaactttggcttcttcagca
tcttcactttacggacataaacatcgtggagaggataaattgactggcaggccttttcaatatctttgcctatgctgtctggaatcagtttattaacaac
ctctttaaggtcgtttgtttgcacttcacgggtcataatttccatcatcttcttgcgaatttgacggacctgctggtgttgagcataggaagtctttctg
atttgattgttgcgctttttagtgaatccaacacagaacaggcggagcaagtagccatctgtggtcttcacatcaacatgtgcttcaatcatggtctgcc
atttcttgaccatggagcacatcttatcacgagtgagatccatgccatggaagttggtcaggcagttcttgccctgaacgtcttctgtgataagcttgaa
tttacggaaagccacttcgtcatttttgcagatcagccaagcttacttcaaaaacgcgacccttcagcccatcaaaggcaattttttgttccttgcgttc
tggtgacaaaatcttttcaaggttgcggatattgaaaattgctggtggcttgacaccaaaccatccttttttggagaaaggggcccaaaccttctttttg
tccccctttttgcccccttttttcacccctcccttctttcccaatgcccccttcaaaaaatgggggaaaaaaaaagggggggggggg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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