XB-CLONE-3914889
Clone Name: IMAGE:7091791
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Gene:
KIAA0753
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654491
[+]
taattaatcccccccccccccggttgtcatggcggtgttttgaaattgtacctgcagaacggtatggaatgcgcgttacaccgtgtaacacatcggggcg
ccgggatcgcagggtttgaagatgacgttagacttccagcattatccaagaccagtccattcagtgttatcggatttatctggagcttggaatatacccg
acaggggaagccaaaagatgaaggaaaatcagctccagtttaatttggatgtgcttgctcacccaaaaaatttggctgtccagtattcaaacccaagccc
aattataatagaaaaacttcgcacatctcagcaacaagcagaagagattacagcagtgcaagatttaggaaattcttcttctgtctctttttctgttgta
tccgaggaacgtctaaaccttgctgttcaacttgcaaagagagatgtgaaaaggaaacatatagaagatagatttaaacccaaagaatatggagtaaagc
cctgtgcttcagaaaatgtgaagaacaaacagaaaacgcaaaaacaaagttggtcaccagcctgtggaccaaaatctgaagtaactagctctggagcccg
ggtgtatgtgtacactgcagatacacaaagaacacagccagaaattcagactctccgcaactcatgatccggacctggcacaggatcccaaaacagacca
aagaggtgagcagagtg
3' EST: CN331033
[+]acctctagcttcttcaacagcatccaacacatctcctgagcattctctttgtgctaactcttggatcttctgtatatacgtgtgcagctctttctgcagg
cgcttcacctcttgctcaccctcctctgctcctgttttggggatcctgttgccaggtcctggatcatgagttggcggagagtctggaatttctggctgtg
ttctttgtgtatctgcagtgtacacatacacccgggctccagagctagttacttcagattttggtccacaggctggtgaccaactttgtttttgcgtttt
ctgtttgttcttcacattttctgaagcacagggctttactccatattctttgggtttaaatctatcttctatatgtttccttttcacatctctctttgca
agttgaacagcaaggtttagacgttcctcggatacaacagaaaaagagacagaagaagaatttcctaaatcttgcactgctgtaatctcttctgcttgtt
gctgagatgtgcgaagtttttctattataattgggcttgggtttgaatactggacagccaaattttttgggtgagcaagcacatccaaattaaactggag
ctgattttccttcatcttttggcttcccctgtcgggtatattccaagctccagataaatccgataacactgaatggactggtcttggataatgctggaag
tctaacgtcatcttcaaaccctgcgatccccgcgccccgatgtgttacaccgtgtaacgcgcattcctacccgttctgcaagtacaaattcaacaccccc
cgaacacggggggggggggggggggggaaaaattattatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: