XB-CLONE-3914863
Clone Name: IMAGE:7092139
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Gene:
ncl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK654264 [+]
taattaatcccccccccccccggtcggttccttgttgcaggggcatcgtgtcgtctggatcctagacacgtgaattaccattctctcccaaacacaacac
cggccaccatggtgaaattagctaagggtgcaaagacacaggctaagcccaagaaggcggcaccaccacccccaaaagatatggaagacagtgaggaaga
ggaggacatggaagaagaggacagcagtgatgaggagatggaactgcctgtaaagaagacaccagccaaaaaggcagccactccagccaaagcaacatct
gcaaaggcagccactccagctaaagctacagtcacaccaggaaagaagggagcaactccagcaggagccaagaatggaaagcaggcaaagaaacaagaga
gtgaagatgaggaagatgactctgatgacgaagctgaagacccgacacctattacaaacaaaccagttgctaagaaagctgtagcaaaaaaagaggaatc
tgaggatgatgaagaggatgatgaagatgagtctgaggaggaaaagccagtttccaagaagccccttgccaagaaccctccagctaaagttacacctgca
aagaaacctactgggaagaaacaggaatctgaagatgaagaagatgagtctgaggaagaaaagctggttgcaagaaccctctgcaaagaacctgcagcaa
aaaacagatctg
3' EST: CN331134 [+]tttttttttttttttgtttttgtcaaaagccatagccttctcaatcttcacctctgaaccaagtatctttttcccactcagctttaaagcctgctcaacc
tcttcttcagatgagaaatccacataaccaaacttctttgagtttccaatccttacatcctgcacagttaggttcttcttggaaaagaactccctcagag
catctttaagctcatcaaaatcttttgaggcgtttaagttgccaataaaaatggacaatccttctgatgtgtctgtctttgctttttttgcctcaggaat
atttttagtctttggcatttcttttttgcgttttgcagacacttgttgttcttcgtcatcctcatcctcctcatcatcatcgtcgtcgtcatcctcgtcg
tcgtcctcatcatcatcatcatcgtcatcctcatctgctttggcagggacagttttcttcccctttagagctggtgcaacttccataggctcgtcttcat
catcttcagattcttcatcatcagattcttgttttttgcctgcaggtttctttgcaggagggttcttggcaacaggcttttcttcctcagactcatcttc
ttcatcttcagattcctgtttcttcccagtaggtttctttgcaggtgtaactttagctggagggttcttgggcagggggctcttgggaactggctttttc
tcctcagactcatcttcatcatcctcttcatcatcctcagattcctctttttttgctacagctttcttagcaacctggttggtttgtaatagggggtcgg
gtctttcgctttcttcctcagaatcatctttccccat
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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