XB-CLONE-3914753
Clone Name: IMAGE:7091131
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Gene:
mthfd2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CK653890 [+]
taattaattccccccccccccttttaggcttagtggaagttacctcccggtagcccttcggtgctcaccatggcaaccctcacatctctccgctttctgt
gctgcgctgtaagagcccaggtccgagcgatccactgtaccccttgcaggaacgaggcagtcgtcatctctggaaggaagctggcaaggcaaattcggca
aggggcacggcacgaagtggaacagtgggtagcagctgggaacaagagaccgcacctcagtgttgttctggttggagacaaccctgctagccattcttat
gtgttaaacaagacaaaagccgctgcagatgtgggcatcagcagtgaaaccatactcaagccaacatccatcacagaagaggagctgttggatctcatca
acaagcttaacaatgatgatcatgtcgatgggctccttgtgcagctgcctctcccagaacacttagatgagagggctatctgcaatgctgtgactccaga
caaagatgtagatggttttcatgtggtgaacgtgggcagaatgtgcctggaccagtactcaatgctgccggccaccccctggggagtgtgggagatcatt
aagagaacaggaatccccactcttggtaagaatgtggtagtagctggaagatctaaaaatgttgggatgccatttgcatgctgctgccacagatggaagc
3' EST: CN331749 [+]gcacgcggttaattccaacatcgatgactgcagcaccttcctttatcatgtcagctgtgataaggttagggatacctgcagctgcaacgacaatatctgc
aatcttggtgtgcatcttcagctgctctttaggagtgtagcggtgagagatagttactgtggcatcacctccagggcgctcgtgccttccatctgtgtgc
agcagcattgcaatgggcatcccaacatttttagatcttccagctactaccacattcttaccaagagtggggattcctgttctcttaatgatctcccaca
ctccccagggggtggccggcagcattgagtactggtccaggcacattctgcccacgttcaccacatgaaaaccatctacatctttgtctggagtcacagc
attgcagatagccctctcatctaagtgttctgggagaggcagctgcacaaggagcccatcgacatgatcatcattgttaagcttgttgatgagatccaac
agctcctcttctgtgatggatgttggcttgagtatggtttcactgctgatgcccacatctgcagcggcttttgtcttgtttaacacataagaatggctag
cagggttgtctccaaccagaacaacactgaggtgcggtctcttgttcccagctgctacccactgttccacttcgtgccgtgcccctttgcgaattttgcc
ttgcagcttcctttccgaaatgacgaactgcctctttcctgcaaggggttacaggtggatcgcttcgaacctggggcccttacaccgccaacacaaaaaa
gcggaaaagaaggtgagggttttccctggggggaagcccccaagggggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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