XB-CLONE-3674418
Clone Name: IMAGE:7795525
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX779768 [+]
ctatgtctttgagatcatcgattcgattcggcacgaggcgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcctctgag
gaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagtggct
tcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatgaggt
gttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaaggga
ggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggggagc
gtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcagagca
gcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtggtatc
tggcacaatgaaacaagaccttcctggtgtgggtaaatgaagaggacatctgagagtcatttcatgcagaaagaggaa
3' EST: CX780254 [+]ggatnncttcggntcggatctcctaaacgactcactatagggcgagaggcttttttagaggtgatattttggttttattgtctccaggcaatcatggggg
gtttacaaaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgattttgaagtttt
tgttttcttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttttgacctcta
atgatcttaatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatca
tatcatcaatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtcaacttcagagaagcc
cagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctca
tcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagat
gttcattccacatgaaggggtgtcctgcttgtttaaagatctcttcaatcttctgaagtccctcacagaagcgtctgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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