XB-CLONE-3674392
Clone Name: IMAGE:7795834
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Gene:
nrap
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780269 [+]
cctgttcnntgggatcctcgattcgattcggcacgaggtacaagtcggatttctgcacacgcggggagtgggctgggaggccgcaggatccccccacatc
gaggcagtgaagaaggccggggagctaataagtgagagtaagtaccggcagaacccgggctccatggctttcacctctgtgcaggactcccccgacctgc
tccatgccaagagcagttacctgcagtgcaatgagagactgtacagatctggggactcgggatcgttgcacagatacaccctgcccccagatcaccccga
ctttatccgagctcgtatcaacgcctcccagatcagcgataaagcctataaaacctcgtgggagcagtcgcgggccgctgggtacgacctgagactcgac
gccattcccttccaaaccgccaaggtgtccagggagatcgccagcgatttcaagtacaaagaagccttcatcagggagaaggggaggcagattgggttcc
gcagcatcagcgacgaccccaacgcccaacacgtcctgagggtcagcaaactgcagagcgacagggagtacaagaagctgtcggaggagagtcgcccccg
agtcagaaccaacctggaccaaccgggtttcatccaagccaaagagagccaggaacaagccagcgacatccactaccggcagcacttgcaccgctacacg
tgtgacccccagcagctagccatgacacacgccaagcaagccacagcctgcagagcgatattagtacaatcagatctgactggatg
3' EST: CX780175 [+]aaagcaagggggagcggagcggcgagcgggatgtcntatacgactcactatagggcgagaggctttcgaatggagtcgcgtttattccgtacaaacgatt
ctacaaagaagcccaaatctcgtgtataaaaactgatcctgcagtcgctcgagctccgatatacgtgacaaacaaacacaggggggtgtcggctctgcaa
ggtcagactcaccggcgatcccggtacaatggaaacactaccagtcaaggaaatgctattaaactacactgggggggcaattaaatgtaaagaaaccaag
gggggcagtttatatgtggcattaacagcataactgactagttccccactagtaatactattatatagaacccccacacattctaccacattctgcctcc
tgcggtggcccaaagtagggcaaagcagggggggcagtttaggacgttccttgtgcacgggggcagaataacccaggcagtcatgataaataccctgagt
tacacacaaacatagcaggagctgccccgttcctttctatctgccccggggaattcccaagcgatcggaccggcaccaaatgttcattttttttttgcca
gaattttcttcctcttcacctgaaggatctcggaggcatcgggattaacatcgctgtgtggggactggtaccccgtctcgtctgctgaataaggagacac
tgacgcccggtctgactctgtgccatctccgtatgacagttgggccgcccggcgagccagttctactttgtagtagccaggggggggtccaaccaacccc
tt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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