XB-CLONE-3674205
Clone Name: IMAGE:7795793
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX779764 [+]
acctattgttttgaggtccatcgattcgattcggcacgagggcccgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcc
tctgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaa
gtggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatccta
tgaggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaacctt
aagggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtg
gggagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgc
agagcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgt
ggtatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctgagagt
3' EST: CX780381 [+]ggggggggggnggcgatgntcnttctgttggactcgtaatacgactcacaaggggcgagaggcgttttatcgtctccaggcaatcattggggggtttaca
aaacaaatggtgaaaggatggtaaatgtgcaataatataatctttatcagagaattagatttatcttctcttttatgttgattttgaagtttttgttttc
ttgtgctggatcttcctactacatttctgagattatcccacccacatccattacttatgtattcccctgtagatggccattttttgacctctaatgatct
taatggggtctcctgtcccctttgatggtggtaagggttagggtgtttaggctctgtctctagcccctgtcatctacttctgtgctggcatcatatcatc
aatggactgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatccactaccatctggacttggtccacttcagagaagcccagccta
tcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatt
tggggtgtttgctgaggttggggagcttggcatggacacctcctctcaggcctgtgcccaagtttgatgggcaggtaaggacataacccagatgttcatt
ccacatgaaggggtgtcctgcttgtttaaagatctcttncatcttctgaagtccctcacagaagcgtctgaagacttcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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