XB-CLONE-3674157
Clone Name: IMAGE:7795443
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Gene:
amy2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX779950 [+]
ctcggtnttgcggatcatcgattcgattcggcacgaggcactcaatctggacgaacatccattgtgcacctgtttgaatggagatgggacgatattgcag
cagaatgtgaacggtatttgggaccaaatggctttggaggtgttcagatttcacccccaaatgaacacattattgtgacaaacccttatagaccttggtg
ggagaggtaccagcctataagttacaagctctgcagtcgttctgggaatgagcaacagtttagagacatggtgacaagatgtaacaatgttggcgtatat
atttacgtggatgccgtaattaaccatatgtgtggctcaggtggaggtgctgggactcattcatcttgcggcagctacttcaatgcaggatccagggagt
tcccagttccctattctagctgggattttaatgatggaaagtgcaggactggcagtggtgaaatagaaaactattcggatgctaaccaggtccgagactg
tcgtcttgtcggtcttcttgatttggcactggagaaagactatgttcgtggaaagattgctgaattcatgaacaatcttattaacattggggttgctgga
ttccgactggatgctgctaagcatatgtggcctggggacctaaaggctatttcagaaagactcaataatctcaatacaaaatggttccctgctggagctc
gcctttcatcttccagaggtattgatcttggggtgaagcattctgtcacgatactatggatncgagagtactgagtc
3' EST: CX780409 [+]gggggcggntctactgtctggtctcgtatacgactcactatagggcgagaggcttttttagctcacttcagtttattaggtaattgcattttagaaaatt
tgtaaatagtttttttcttaggtcactgcacttaacatagtgcaggggtatattatatgagatcatgtttttggaactttataatttggcatttacatga
atagcagcgaatgggtcttcgtcgttgttgcttatttggaatttagcatagccattgccatccacattgatctgcctgcctgtgcagcgagatccctcct
tctgtccagagatcacatcacagtaggttccacttggaaggccagtattcagagtgacatccaattgccaatcatcattattaaagattatgaatccttt
attgccacgtccaaatgcaacttggttgctcccattatcccaccagtttgagaagggttgaccatctgctacattacggaaggtaaccatgttcttaatc
tgacgccatctatgctcacagacccagttgtcaccacatgttgagtcagggttgattgggactgatttggtggatccatcggaattactaggagggccaa
tccagtcattttgatcctttccattgactatgttcctagaccatctgaagctagacatgacacgagtaaaaccataaggatgtgccagcatgagccaact
ccattttgtacagacgtgctcccagaagtgagtatggagctcctcagcacgtgtctctctgatatcagatgtcacaacacangctctgtcatangatgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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