XB-CLONE-3674136
Clone Name: IMAGE:7795843
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Gene:
sdc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780420 [+]
ctagttctttgcagaccatcgattcgattcggacgaggaaaaatgtcgccaacctttatgcataaacacacaaacgacaatgggaaatggtggcgccaag
cttcgggtcaatgaacggttgtttaatatttttagcatgtctcagatccttcaaattttagttttatttcattaatttatctgtttccaaacacaatgcc
ttttgtagttgtcatggtgcaattgtcttagggtaattcagatatacagtatataagtgtaaatgttttttttttctttcctatgtaaacattaacctgc
gaggatttgtataaaactgtaacgatattcattttacaccattgttctgttttctttgtttctcagtgttctttgggcaaatttaacacgatgagtgtgt
aaaattggaatatcccccctctcttccccaaccacaaagctgttaggtgaaagccacgtgggtaagaacgattgttggctgctcgtatttgctgcaacca
atggcattgtttggattcagctatatacaaattgttaacgaccattatcccaagaggcagacattggctgcctgctgtaaagatacaattctatccaatg
ggagtatttgtattcggaagaaattcaattttccaaacaaaaagtcacccacgtggcctcagccttagtaagctccccttaaataaaggtgcattctctg
ttcatatttgccatttcactgccgaaaagaccaacaagtccttaccatcttgtagatgtctctggtcaagagttctgttgtttt
3' EST: CX779824 [+]aaaaaggggnggcgatgactactgtcggactcgtaaacgactcactatagggcgagaggctcgagttttttttttttttttttgattaggccacatgatt
ttattcagtagtgtttatgttcccaaattacattttaggtctattgatgtaagactacaaaattaccattagatttttctgtgcttataaaataccaaca
tattttttttgttcactgtcatacactctgtaacacagtctgtaacaatttacatggaatcataggcacttcaaaggcttaaaaagactcttacaactta
aaatgcgtgttgaaaaaaaaaaaaaaaaaaaaagacaaaattgcgctggaaactcccaagtcgtaccttgaaaatgtaaaggagttgaatacagtggcca
cggacatctgcaactttgtcagaatactgttatagtctttggacttggttcaaccttcatcctctgtccaatatcacatagcaaatatggcaagaggcac
ccgtaggacatcctacgcaatgctctgctggccctctccaacggataatggtattaaaggattcatctgctccctaaataacatgggtttaaaaagcaat
gtgtttattggcttactaaaacctgtagtatacctggttttatatgtctctaaggcatagcatgtaggtgcccaacaaaccaactgcttccttatgttca
tccctggtactacactcttaaatgagtcctctgttgcacaatacatggcggagtctttacaactccaaagctctggactttcattccttttcata
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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