XB-CLONE-3673974
Clone Name: IMAGE:7795590
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Gene:
ckm
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780512 [+]
cccatgtctttgggaccatcgattcgattcggcacgagggcccgatcttccacatgcctttcggtaacacccataataactggaaattgaattattcctc
tgaggaggaatatcctgacctcaccaagcacaacaatcatatggccaaggccctgtctgctgacatctacaagaagctcagggacaagcagacaccaagt
ggcttcaccttggatgatgtcattcagactggagttgacaacccaggccatcctttcatcatgactgtgggctgcgtagcaggggatgaggaatcctatg
aggtgttcaaggacctttttgatcccattattgaggatcgtcacggtggttacaagccaactgacaaacacaagacagacctcaactttgccaaccttaa
gggaggtgatgaccttgacccaaactatgtgttgagcagccgtgtaaggactggccgtagcattaagggatatgccctgcccccccactgcagccgtggg
gagcgtcgtgccattgaaaaacactccatcaaagctctcagcagtctgtctggggaattcaaagggaaatactaccctctcaaggatatgtcagatgcag
agcagcagcagctgattgatgaccacttcctgtttgacaaaccagtgtcccctctgcttctggcctctggcatgggccgtgactggcctgatgcccgtgg
tatctggcacaatgaaaacaagaccttcctggtgtgggtaaatgaagaggaccatctgagagtcatttccatgcagaaagggagaacatgaaaga
3' EST: CX779773 [+]ggaagactactgtcggatctcgtaaacgactcatcacggggcggaggctcgaggttttttcccctctttctctttttttttttttttttttttttttttt
ttttttttttttttataaaaggggaaattttgggtttattggccccaggcaatcatggggggtttacaaaacaaatggggaaaggatgggaaatgtgcaa
taatataatctttatcaaaaaattaaaattatcttcccctttaaggtgaatttgaaggttttggtttcttgggctggatcttcctactacatttctgaaa
ttatcccccccacatccattacttatgtattcccctggagaaggccattttttgacctctaatgatcttaatgggggcccctgtcccctttgaaggtggt
aagggttagggtgtttaggctctgtctctagcccctggcatctacttctgggctggcatcatatcatcaatggactgtcccttctcaagcttcttctcca
tctcaatcatgaatttcacaccatccactaccatctggacttggccaacttcagaaaagcccagcctatcagcattggagatatcaaaaacacctcctac
tgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaagcctgcccagtatctcatcaaatttggggtgtttgctgaggttggggagcttggca
tggacacctcctctcaggcctggcccaaatttgatgggcaggaaggacataacccaaatgtcttcccatgaagggg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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