XB-CLONE-3673803
Clone Name: IMAGE:7795137
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Gene:
atp1b1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780567 [+]
cctacatgtctttgaggtccatcgatcgtgccgatcggctttccatacccgcatacagtaaccagcgtgacagtggccagccagtcccattgttagataa
caggagaattgtagggacaaggaagaagaagcagcccagtcacgcttcccatcagcgcccagtgacatctcaatagccggtaccgcaaaataacacagaa
cccttaactgaagtaggacaatatggccagggacaaagccaaggaaaccgacggcggctggaggaaatttatctggaatcccgataagaaggaatttctt
ggcagaacaggtggcagctggtttaaaattcttctgttctatctcatcttctatgggtgccttgctggcatatttattgggaccattcaagtactcctgt
taaccatcagtgaatatgaaccaaagtatcaagatcgtgttgcacctccaggactgacacaagtacccaaagccgtcaaaactgaaatcaacttttcanc
aaacgatcctgacagctacaatgattacgtgcaaagcatggaaaagtttnattcgaagtaccagcacgagaacccagtatccgataagttgaagaatgtg
gcactatgcctgggccataactgagaaggggggtcttaaccaagatgggggaagaaaaaatccgtgtgttccgactcaagggtgccaacggtctggctag
atgtccacattggatttccaagggagcatggtctcctgactcaacaattg
3' EST: CX780609 [+]gcggacntactgtcggatctcgtaaacgactcactatagggcgagaggctttttttttgtttattgttttattgtctcatgtttaacttgactgaagttc
caacaagagcacaggactcctggcaattattacagatgtatgaacaatgtgtgcacagtagacatttaaatacatcctatgggatactgcattgccatga
acgttgttttaaaataaagatttatttttcaacaggttactttggtttgtttcatacattttctttttcttgaagaaaaataaaaattgtacaactgcat
aaatataaaattcttccaccatgaaaatggttaaaacatttgtaaagacaaagcaccaaactgtgatgctttcagaaaaaacaaaaaaacaaaaacataa
aataaaaatatttatatatatataactcaaaaaccattagccctatttctgacaaaaagtgacagtcggacccggggcttcacacaccttcataatgggc
ttaagcatttaaaggggaaagtttctataaaaagcattaaataaaaagcaccgcacaacccatcacctcttgcatttcagtgaaactaagaagagaaaga
caaaaacagaagctcttttacagataaaacaaatcaatgccttatttaaaggacttgtggagtgctattaaatattattggttggccaccacttccgttt
aaattaaaaaaagaaaaaacaaaacagaaaagtagncataaaataccatgccagttttatccccatacagactattacacctggactacaacttgctcac
aagcagaacaaacaaacatggctggccgaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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