XB-CLONE-3673780
Clone Name: IMAGE:7795738
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Gene:
vapb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780621 [+]
aatgggnncctagtttntttgngaccatcgattcgattcggcacgaggtgacattgtacagtgtgactataaagagttaaccgtgacgctcgttaatttg
aatcggactcttcctaacgaatcgtccccgggggcaattaaacctgactctaaaagcgaatgttacggaggcgcgaggggagggggtggtgtggacaccg
tcacttccgctgttacatctcccctagtacctttttccgctcatttcttgtggggagtctatagacctattccgccttggatgtatgccctaacgcctcc
tctctgacagtataagcttcagctgggacgattaatcccatgccaatatctccaatggcccctatagctatatacctgtgatgcatgcttcccgcaccta
ctcgtgcagctcccatatcaacgttgattctgcaccgtggcataaaagcgaaccattttactggaatgctaatcacctggaactcaccacgcgtgtgtag
atcggacaaatacactctttaagaacacaccctgttgtaactcttcaccctccatcagacgatgcccttgctttgaatgatactctctatgttgtgtgcc
ttgcggctggatctaatcctaaaagcatctcaattaaatggctgcttaatggctagcagacaacacatcgtgtctcgtttgcatagcccactgtagacaa
caatgtaggatctgagactacatcctatatctccttaactagacaggatgggatttgaaactactactcatgtgactcgacatgtgattt
3' EST: CX780404 [+]tcggatcacgtatacgactcctatagggcgagaggcctttgctgcatcacccgctcctgcagccgccaccgccgccgggacaggcccggacccccagccc
acacgggagacatggccaaggtggagcagatccttcagctggagccccagcatgagctcaagttccgaggacccttcacagacgtcgtcactacaaacct
gaagcttggcaaccccacagacaaaaatgtgtgtttcaaagtgaagaccacggcgcctcgcaggtactgtgtgcgacccaacagcggggtgatcgacgcc
ggatcctccatcaatgtgtccgtgatgctacagccgttcgactacgaccccaatgagaagagcaagcataagttcatggtgcagtcgatcatggcaccga
cagacacctcagatatggaggccgtatggaaagaagcaaagccagacgacctcatggattccaagctccggtgtgtgtttgagctgccgtcagagaatga
aaaagctctcgatggagagataaacaaagtcatttctagcagtataacaaagactgagtcctctctgtccaaatcaataagctccaatctggatgattct
gagtataagaaagtggtggaagaaaacaagaggctgcagacagaaatgcagaggctacgggaggagtacaagcaatttaaggaagaagacggtttgagaa
tgcgaaagatgcagcccacaagcagcccaaccgacccgtctccggctcgcgaagaagaagagtgacactcg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-ACTAGTGCGGCCGCCTAGGCCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 1.5 kb. The original library contained 1E5 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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