XB-CLONE-3673713
Clone Name: IMAGE:7795221
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780066 [+]
catctnntttgagatcctcgattcgattcggcacgagggttcaggtcggctttggaagagtcggtaaacatttttggagtttccaactacaaggagatga
ttttgtaccagatattgtcaccatggggaagccaataggcaatgggcacccaatgtcttgtgttgtaacaacgaaagaaattgcagaagcctttggggca
actggaatggaatactttaatacgtttggaggaaatcctgtatcctgcgcaattggactagctgtgctggatattattaaaaaagaagatctcagaggga
atgcgaccagagtagggaattatttgactgagctactgaatgagcagaaacaaaagcacccattgataggagacatcagaggagttggcttgtttgttgg
agtagatttggttaaggacagactgcttagaactcctgcaactgctgaggcccagcatataatatataaattaaaagaaaagagaatcctactgagtgct
gatggcccttacagaaatgttctgaaattcaagcctccaatgtgctttaccaaggaggatgctaaactggttgtagatgagattgataaatgccttacag
ttcttgaaaaggcaatagaaggccaatcaaattctggtatacatgaaaaaaccgcagccaagagaaaggtgcataatgaaattcangagacctaacacaa
aagagaggaacatgtacagcagctacgagaagcacctaatgacatgctacagacagcacgactgcatctggg
3' EST: CX780656 [+]gcaaaggggnntggagagnaanntgtcggatctcntaaacgactcactatagggcgagaggctttgactagatgtgcagaatttaatgaataagctttta
tttcttattatgttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggatttagccaagcagtaggatctggtataatgtg
attatggatatctgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatgttctgacactttaagaaaagaagcatacg
catgtttacctagttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattatagcaccttgtatgttgacaaaaaagaaaa
taccataggcagtgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatataggaatcatgtacacagggatttcaggctgc
tacatcagatctgtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttgagcaagagctaaaatcttggtgtatctaa
acagttggaaacggcttaaaattttcttgtacatagcatgctcagtacattcagcatgctgaggtcttttaaactgcatcttaaatctaagcccattaaa
catgggaaaagattccattctcatatgatatactgaaactgncaatacngaataaaaaaaaataaaggatgcacacataaaccctaaaatcatttgcaat
caatttnnacaccttcgcagactgaccaacatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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