XB-CLONE-3673590
Clone Name: IMAGE:7795325
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Gene:
ush1c
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX780155 [+]
ctttacgtttttgcggatcatcgattcgattcggcacgaggaggaaaaggagcgaatgagaaaaatggagatttcccagaaagtggccgaggaggaggat
cggttccgccgggagatagagaagatttcttcccaagaggagaaaataaagaaggattgggatgaagactggggccccaaagaggaagctaaaagcgtga
agcccccgggggccaagacacagcgcagccccccagccaagagcaaacaagatgctgccaataagtttgcggggagaagcaataagcagaagtacgtggg
ggactttgatccttacaccatgttcacggcggatcagattggtgggaaggacgtccggctgctgcgaataaagaaggagggggcgctagacctggcagtg
gaggggggaatcgagtcgcccattgggaagattgtggtctctgccgtgtacgatgggggtgcagccgacnaacacggtggcatcgtgcagggggacgaga
ttctggccgttaatgggaagatactgaccgacgtgacactgacggaggctcangcnacgctgacgaaagcttgaaacagggaagggactggatagacttg
tcattcctgtttcgcccccaaggatatgatgatgagtaaccgtatttgaggagaactgcaatcacgatcgccggagaggtctactgtgccggtctgcgga
gagagcctatgtggaaccgaatcatgggttttccgtgatctctggctcggaatgattt
3' EST: CX780720 [+]aagcaaagggggntttatagagncngtcggatcacntaaacgactcactatagggcgagaggctcgagttttttttttttttttttggtatttccatatt
tattaatgtaccgaatgtgacaatgacagttggacccggtactgtggggctttggcccagtaatagtccgtatggatcagttccgcacattaatattgat
ccaacgcgttgatcttggggtaaaatcacgtcccgagcgcagagatttcacgggaaaaactcattgaattctgtgttacagcaattaggctcctcctccg
cagaaccggcacagtaagaccctcctccgcggatcctgattggcagtttctcctcaaaataacgttacttcatcatcatactccttggggggcgaaacag
cgatgaccaagtctatccagtcccctcccttgttccaagctttcgtcagcgttgcctgagcctccgtcagtgtcacgtcggtcagtatcttcccattaac
ggccagaatctcgtccccctgcacgatgccaccgtgtttgtcggctgcacccccatcgtacacggcagagaccacaatcttcccaatgggcgactcgatt
cccccctccactgccaggtctagcgccccctccttctttattcgcagcagccggacgtccttcccaccaatctgatccgccgtgaacatggtgtaaggat
caaagtcccccacgtacttctgcttattgcttctccccgcaaacttattggcagcatcttgtttgctcttggctggggggctgcgctgtgtcttggcccc
cgggggcttcacgcttttagcttcctctttggggccccagtctcatccaatt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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