XB-CLONE-3672251
Clone Name: IMAGE:7640756
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Gene:
mitf
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC135968 [+]
AGAGAAACAACTGAAACTCCCGTATGTATAAACTAAGTGCAGGCAAGGACGAGGGAGTTAGCAGTGCCTGACGGTGTGAGGGGAGAGTTGGCTGCACCCA
CTCGGAGCTGTTTCACAGACAAAACACCCCTCTAGGACACAGGGCTGCGGAAATGCAGTCAGAATCTGGAATTGTGCCTGACTTCGATGTTGGCGAAGAA
TTCCACGAAGAGCCCAAGACTTATTATGAGCTGAAAAGTCAGCCGCTGAAATACAGCAGCTCAGCGGAGCACCCCGGAGCTTCCAAGCCTCCACTAAGCT
CCTCCAGTATGACATCACGGATTTTGCTACGACAGCAACTGATGCGTGAGCAGATGCAACAGCAAGAGCGACGGGAGCAGCAACAGAAGCAGCAGGCTGC
TCAGTTCATGCAACAGAGAGCGGCAGTCAGCCACACGCCAGCCATTAACGTCTCCAGCAGCCTGCCTGCTTCCCCCCAGGTACCAATGGAAGTACTCAAG
GTGCAGACCCACCTTGAAAATCCTACCAAGTACCACATCCAACAAGCCCAGAAACAGCAGGTAAAGCAATACCTGACTACGGCCAAACACTCCGGCCAGG
CGTTAAGCTTACCCTGTCCAAATCAGCCTGGTGATCACATCATGCCCCCTGGACCGGGAAGCAGCGCGCCAAACAGTCCAATGGCTTTGTTAACTATTGG
TTCAACCTGTGAAAAAGAGATGGATGATGTGATTGATGATATAATAAGCTTGGAGTCCAGTTATAACGAAGACATTCTCTTGATGGATCCTTCTATGCAA
ATGGCAAACACGCTGCCTGTCTCTGGTAATATGATTGACATTTATGGAAATCAAGGTATGCCTCCTCCGGGGTTAAACGTCAGTAATTCTTGCCCTGCTA
ATCTGCCCCATATCAAAAGGGAGTTCACAGAATCGGAGGCAAGAGCGTTGGCCAAGGAAAGGCAAAAGAAGGACAATCACAACCTCATTGAAAGAAGGCG
AAGATTTAACATCAATGATCGCATTAAAGAATTAGGAACTCTAATACCCAAATCGAACGATCCTGACATGAGGTGGAACAAGGGAACGATATTAAAAGCC
TCGGTGGATTACATTCGCAAACTGCAACGAGAACAGCAGCGGTCAAAGGAGGTCGAGAACAAGCAGAAGAAACTGGAGCACGCCAACCGCCATCTGCTCC
TGAGGATACAGGAGCTAGAGATGCAAGCTCGCGCACATGGCCTTTCCATTATGCCTTCTACTGGCCTCTGCTCAACAGACCTTGTCAACAGAGTCATCAA
ACAGGAGCCAATGCTCGACAGTTGCAATCAGGAGATTCTGTCAAACCACCACGACCTTTCCGGCACAACCACTCTGGATCTCACTGATGGAACCATTACT
TTCAGCAATAACCTGAGGAACATGACTGACTCCCCAACAGCCTACAGCATCCCCACCAAGCTGGGCTCTAAACTGGAAGACATGTTTATGGACGATACAC
TTTCTCCAAGAGTAAATGATCCACATCACACCATATCTCCTGGGGCCTCCAGAACAAACAGCAGAAGAAGCAGCATCAGCATGGAAGAAAACGACCACTG
TTAGCACAAGCTACAGGACATGCTCTTACAAACTGTAATACTTTTATTGTTTCAGAGCGGTAGATAACTATACTTCTAGCTTTCTGGATAGCTCACTTTC
ATAGAAACTTTGTTGTGTTCGCCCTATAGGCCAGCACTTATATATATTTTATTTTTTTTAGACTTTTGTAAAGATTTTTTTGGTGTATATCATGACTTGG
TTTGCCTCCAAAGTATTTTGAGAAACTGCTTTGTACATACCTGGACTAACGGAGAATGTCAAACCTATTTAAAGCAGCTTCCCTCCACCACAACCATACT
GTCAAGATATTCAGGTTAATCTCTCAACAGACCGCGTCTCATTGTCATATGGAAATAAAGGCACAGAAACAAGTATCTGATTGGAAGTACGTAGAATACC
TCAACCCATCTGGGTGATATGGTCCTAAGAAAATGATTATACATGACTCTGAGAATATATATGAAGATAAGACAAGGAAGAGCACTCAATCATGTTTTTT
TCCTGGGCCCTTTGGTTTCAATAGAATAGAATCTGGAGTAAAATGAAAAAGTATTATGGCAAATGTTTAACGGATCAACTGAAGAGAACACATGGTTTAA
ATGTCAACTACTTGTATTTAATTGGCACTATACATTGGCAGGATATGTAGCTGATAAAAGAGGCTGATTATTGAAATATTAGGTGTGTAGTATGTCTAAG
CAACAATATTTACTGGGGAAGTTTGGAGAATTAAGATAGGACAAGAAATGCAAGAGATGAGGACTAGGGGCACAGGTATAGGTGTGTGACAGTTTTGCCC
AAGTGGGGAAGATAATACTAGGCACATATTGTTTTGTTGAGGGAGTCTGGACTTAGACTGGATTGGTTTTAGAAGTGATAAAATTTCCAGTACAAGAGGT
GGAACTTAGAACAAAAGATCAGGCATAGGCTTCTATATCCAGGAGGGTTGTAGAGGACAGAAAACAGTTAAAGCTTATGTCACAATTGTCGGATTGTTCT
ACGCAAATGCAATTCACCAAAAAGTGCTTAAAAGAATGTTGTAGGGTCCCTGAGTTACCATCACAGAAGCTGACATAGGGATTTAACAGTGGCTTCATTG
TGTATTCCCATTCTGTCATATGATCTTGGCATGTGAGAATGAGCCTTACCATTTTTGTCTTTACTTAGAGGTCATTCATTTTCCTATACAGCTGTAGAGA
GGTGGCCATTTATGGAGGTTTGCTCTGATAACGATGGGTTGAAAACCACTGCAATTCTGGGACAGCTTGGTTGAGGGTGATAATATACTGATCTACCCTA
TAGTGGGAAGTCCTACACAATGTCAGTACATGCACACACCCCTTCTGTTGACACAAACTTTCCAGAAAAGTCCACGTCTTAAGGGAGAACAGAGAAAATA
CTGTCGGAACCAACCTTATGTGTGACTATATTTTCCCTCTTATTTCTGCTAATGCCATTAACAGTATTCCCCCAGTGAGTGACACTAGTCACAGTGTTGT
GAAAGTATGTAAACATGAGCCTCTATGGACCCATTTCTTCTACCCAGATGCTGAAATAGAGTAAATCTCAAGGTCAGCTTACCAAGAACAAACATCTTGT
TTACAAACGTTGATAACGGAAAGGCCTGTTACATCCACAGACCTGTTTCCATAGTTAGAAATGAAGAGGCAAATGTTGCAGGCACGTCTTTATCCTCATG
GGTGGGTGCTTTCATGGGAAGGTGCCATTCTCACCCTTGCTTTAAATGGAGTTGCCAGTAAGCATCAATATCAGACTTGGTCACCTATGGCCAACCAGAG
GACCTGATTTTCTGAGCCCACGGATATGATATATAGAATGTGCCAAATTGTACAGGTTTTTTAATAGGTCCATAGTAGAAAAAAGGGTGGTTTGATGAGA
CTTCTCCTAGGGTGGAGTCCACTAGTCCCCACCAGGAGATCCTCTGATGTTCTTGTAGGCCAGTCTGACCATGATCAGGTCAGTAATCCAACCACATTAT
AAGCCATGAGTGTTTGCTGTGTTTATCTATGGCCCGTGCTGTTTTTCAGCAGATTCAGGGTGTCTAAGCAATGCTCTTTACTGCCTATCAGGGATTGCTA
CAGTATGTATATGTTATAATATAAGTATTTCTGACAATATATCCCTAATCGGCTGCTCTTTGCTTTCAGGGTGTGTGCACACGGGTATCTTAAATTATTA
AGTAAAAAAAATAAAATAAATGCATTGATACATACCACATACCAGGATGCAATGCATGTTCAGAATCCATATAGTATGCATCAGGCAGCTTGGTATATGG
CATTTATTTGTGATTACATATACATGATTACTTATATTAAGGCATGCAGTCATTGATGTGGTGTGTTTCACGCCAATGAAACCCTTGAGGTTGTTTTATT
ATTGGAATTAGTTTCTATGCATTCAGTATGCCCCCTACAGACATTGGGGTGCTAAACGCTTAGAAACCCAAGGGTTCCCTACTTAGAACACTGTCTCATT
GTTTTCATTTCATGTTTTAGCCCCGTACCCTGAGTTTTGGGGACAATGATCTATTTCACACACGTCATGCAGTAGTTTAGTTAGGACTGCCCAAGTCTTT
TGTGGTCTTAAACCCTCTTAGAAGAGGTTGGCCAGCAGTCTCTTCCCTCTGCTGTCTCCCTATACCGTTAAAAGTTAACCAATAATGGATTCATAATATC
TGAGGATGGGGTTGTGGTATTTCTTCCCCCCTTTTCTTTCTATTTAATTAACTGGCCTGATGGTGATCACAATAAACTGGAGACATATTAAGCCCTTCAT
TTGGCTTTACAGGTATGGGACCTGTTATCCAGAATGCTCAGGACCTGGGGTTTTCCAGATAAGGGATCTTTCCATAATTCGGATCTCCTACCTTAAGTCT
ACTGAAAAATTGATTTAAACAGTAATTAAACCCAATAGGATTGTTTTGGCTCCAATAAGGATTAATTATATCTTAGTTGGGATCAATTACAGGTACAGGT
GTAGGACCCATTATCCAGAATGCTCGGGACCAAGGGTATTCCGGATAAGGGATCTTTCCGTAATTTGGATCTCAACACCTTAAGTCTACTAAAAAATCAA
TAAAACATTAATTAAACCCAGTAGGATTGTTTTGCATCCAATAAGGATTATTTTTATCTTAGTTGGGATCAATTCCAAGGTACCGTTTTATTTCTACATA
GAAAAAGGAAATCAGTTTTACAATTCTGAATTATTTGATTATAATGGAGTCTATGGGAGACGGGCTTTCCGTAATTCGGAGCTTTCTGGATAATGGGTTT
CCGGATAAGGGGTCCGATACCTGTACTGTTTTATTATTACAGAGAAAAGGGAGTCATTTAACCATGAAATAAACCCAATAGGGCTGTTCTGCCCCCAATA
AGGGGTAATTATATCTTAGTTGGGATCAATTACAGGTACTGTTATATTATTACAGAGAAAAGGGAATCATTTAACCATTAAATAAACCCAATAGGGCTGT
TCTGCCCCCAATAAGGGGTAATTATATCTTAGTTGGGATCAAGTACAAGGTACTGTTGTATTATTACAGAGAAATTACAGAAAAAAATGAAATAATTTTT
AAAAATGTGAATTATTTGATTAAAATGGGGTCTATGGGAGATGGCCTTTCTGTAATTCGGAACTTTCTGGATAATAGGTCTCCGGATAAGGGGTCCGATA
TCTGTATAATAATAATATATAGAGTAAAATGTATCATTTATGGAGAATCTGTCTCCCTTATTTTAAGACAAGAGCCAGTTTATACAGAGATTCCTGGAAA
TATTTATAAATGCAATTCCTATTTCATAGTCGAACATTGTACTCCTCACTGTAAACTTCATTTGAAGATTTACACTTGTGGGTTTAGGGGTCAGCAATGC
GTACAGTCAGTGTTGGACTGGGGGGTGCAGGGCCCACCGAGGCTTCTGCCTCAGGGGCCCCTGCACCCCCCAATGGCCCCTGCTGCAGCCTTCACACACA
CATATATCCCTCCCCTCCCACAGGGGCCCCCACCACCCACCCAGTCCCCTCCCCTGAGTGCACGTAAATGAAAGTTACCTGCACCGTGTTGGGGGGGGGG
GGGGGGGGGAGATGGGTGGCTCAGTGGAGAGCCCTGTGGTGATCGGGTCTGGGCCACCAGGGCCCACCGGGTTTTTTCCAGAGTCCCGGTGGCCCAGTCC
GACCCTGCATACAGTTTATTGGCTATGCCTTACTAAGCCCTAAGCCCTCAACCCATTTACTATTATTCTTAGCGTTTTAATTTGAAATCGTCCAATATAG
GTTAATTCGATTATAATACTGATTATTTTGTATGAATTTTTTTTAAGGGGGATAAAGCGCCTTTTTTCTATTTAACTGTTTAAAAAAAAGGCGCTTTATC
CCCCTTAAAAAAAA
5' EST: CX794557 [+]agagaaacaactgaaactcccgtatgtataaactaagtgcaggcaaggacgagggagttagcagtgcctgacggtgtgaggggagagttggctgcaccca
ctcggagctgtttcacagacaaaacacccctctaggacacagggctgcggaaatgcagtcagaatctggaattgtgcctgacttcgatgttggcgaagaa
ttccacgaagagcccaagacttattatgagctgaaaagtcagccgctgaaatacagcagctcagcggagcaccccggagcttccaagcctccactaagct
cctccagtatgacatcacggattttgctacgacagcaactgatgcgtgagcagatgcaacagcaagagcgacgggagcagcaacagaagcagcaggctgc
tcagttcatgcaacagagagcggcagtcagccacacgccagccattaacgtctccagcagcctgcctgcttccccccaggtaccaatggaagtactcaag
gtgcagacccaccttgaaaatcctaccaagtaccacatccaacaagcccagaaacagcaggtaaagcaatacctgactacggccaaacactccggccagg
cgttaagcttaccctgtccaaatcagcctggtgatcacatcatgccccctggaccgggaagcagcgcgccaaacagtccaatggctttgttaactattgg
ttcaacctgtgaaaaagagatggatgatgtgattgatgatataataagcttggagtccagttataacgaagacattctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: