XB-CLONE-3667054
Clone Name: IMAGE:7644401
|
Gene:
tulp4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX800380 [+]
cagaagacactagacaggcaggggaagctccgtttctgcaaaagggcatctgcaagaattcactaaagccgattctgattcaaatgaattatcttcaatt
aaccactgcaataagctaactagattgcaagctctggactaattgtgtccattttggcaagcaaaacatgtacacaaactgcactgccagaattcttctt
ccttgatgttttggttcaaaattaaatggggcagatttcccctttccagtttaaatagctcaaacaagggcattgtgcttgatctcaaactttacaaaaa
aacaaaggattagatatttaaatgaactgaaacagaccaataaagagcagctgctaatatttggccatggactggaagaaagcaacttaaaatactgaga
cagagaactcatcatttaaatgacatagagacttcactgtgcattgggcaaactgggcaattagattcatgtacatttctatttatgtcaaaggtctatt
tacttctttgaatggcttaaagagacccttgttttctcctgtgtcagcctgttccagtaacattaagtaaaaatacaaggactgtcatagaaatgagggc
tgaaaaaaactaagacagcagataacatcaaaatagattaacagcaccatgaaaaggtgaaatcttcgttgtcctttagttaaattgtggaggggaaatt
gactactacaactttttgcacttaatccatttcctgttttataactttgtttttagttgggtacaacattggcaaatcacttttttagaaaaaataaagt
gaaataattaaaccatttttatcaccgttctaactcaaattgaaat
3' EST: CX800379 [+]gcccacttagaaaacatttttaatgcattcaagtattagaaaatatcatttagttgcaaattttgagtttgtggtactacaaacagaaaaaaaacaggtt
taatgaaccatgtaggtcattgcttaacaacaacaacaaaataatatataaataaggcagtttttcatttaatattcaaacatctattgcactgaacaca
acagaaccatcaatacaagccaaaagccacactcccaccttaacataaaatggctcccacccaaatatcagcaatattgcatgtaaaaaaacaatgaaaa
ataaaaataaacaacaaattctaagaagatgcctctgtataggcatttgttcttattttccagttttaatctttgcgcttttccacttgcactccacatt
gtgttcgttcccgtataaatcataactgaaatgtttctggtaaggtcaaagcttgactcatttcagacgctgagtcacattagccaaagcaactgcaaag
gcttggacggctgaaaatggatactgaaagtccaaaatatatgcattgccatcaatcctgccaaactgcattacctgtcttccttcaagttcaatctgaa
aatttttggcagattcttgagtcactctgcctccaaagtccaactgatagacctgtgtagcttcgttccatagaggttgtttatttgccataacataaac
aaacccttgactccccaaacgtccatcatctttttcattagcctttcggcacttaaattcatctagatcactcgctgtcctaagattctttttgttttca
gccttttttatgctctggctctggttcatcagttcatctccacttataaaaagttcggcctcactctctgaactatcctgaaactca
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: