XB-CLONE-3661708
Clone Name: IMAGE:7647789
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Gene:
fzd3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC124561 [+]
GTCCGCGGCCGGTGCGTGTGGTGCTCCCATTGCTCCGTGTGAGAGGGGAAACCTGTCAGCCATAAGGGGAGGAAGGAGCGAACGGTTATTGGGCTTTTGG
ACCTTAAAATGGAGACAGTTGTTTGGGAGTACGGTTAAAAGAACGGGCAACAGAGCTTACATTCCATCCTTGGATACCATATAGCAACAAGATGAAGTCC
GTTGTAGCGAAGAGGAAGACAGAAGAAATATGTTTATTCCTAATGTCTGTTCTCCTGCACGGGGGTTACTGAAATTTCCGTAGTCAATGAAACTTGGAAA
CCTTCGGACGTAGGACATTGGCACTTAAAGGGCACATGTGGCTTTGCATTCTCCCATCATGGCTGCCTACGTGATTTCCTGTCTTTGGTTATCGGTGGTA
CTTGCCCAGAAAAGCATGGGGCACAGTCTGTTTGCCTGTGAGCCAATCACACTAAGGATGTGTCAGGACCTTCCTTATAATTCCACCTTCATGCCGAACC
TTCTGAATCATTACGACCAACAAACTGCGGCTCTGGCAATGGAGCCTTTTCACCCCATGGTAAACCTGGAATGTTCCCGGGATTTCCGCCCATTCCTGTG
CGCTCTTTATGCGCCGGTGTGCATGGAATACGGACGCGTGACGCTGCCCTGCCGCAAGTTATGCCAGAGAGCCTATAATGAGTGCTTCAAACTCATGGAG
ATGTTTGGTGTGCCGTGGCCAGAAGAGATGGAATGTAGCCGGTTTCCAGACTGCGATGAGCCATACCCCCGAATGGTGGATATCAGCCTGTCCGGAGATG
TGACCGAAGAAACCCCTTTGGCAGTTCAAAGAGACTATGGCTTCTGGTGCCCCAGGGAACTCAAAATTGACCCTGATTTAGGCTATTCCTTCCTGGGAGT
ACGAGACTGCTCTCCGCCCTGTCCGCACATGTACTTCCGCAGGGAAGAACTCTCCTTTGCCAGATACTTCATCGGCGTCATTTCCATAGTTTGCCTCTCA
GCCACCTTATTTACTTTCCTAACATTTCTTATCGATGTCACAAGATTCCGTTACCCAGAGCGGCCCATTATATTTTATGCTGTCTGTTACATGATGGTGT
CCTTGATTTTCTTCATTGGGTTTTTACTGGAGGACAAAGTGGCGTGCAATGGTGCAAACCCGAGCCAGTACAAGGCTGCCACGGTAACCCAGGGGTCCCA
TAACAAAGCCTGTACGATGCTCTTTATGGTGCTTTATTTCTTTACCATGGCTGGCAGCGTGTGGTGGGTTATTCTCACCATTACTTGGTTCCTAGCAGCA
GTTCCAAAATGGGGGAGCGAAGCCATTGAGAAGAAAGCACTACTGTTCCATGCTAGTGCGTGGGGCATTCCTGGGACCCTAACGATCATCTTGCTCGCTA
TGAACAAAATTGAAGGCGACAACATAAGCGGCGTATGCTTTGTTGGCCTCTATGATGTCCAAGCCCTGAGGTACTTCGTCCTTGCCCCCCTCTGCCTGGA
TGTTGTTGTCGGCGTTTCCCTTTTGCTCGCCGGGATTATTTCCTTGAACCGAGTCCGGATCGAAATCCCCTTGGAGAAGGAAAACCAAGACAAACTAGTG
AAATTCATGATCCGCATTGGAGTATTTAGCATTCTTTATCTTGTGCCACTCCTGGTGGTGATTGGCTGCTATTTCTATGAGCAAGCCTACCGAGGGGTGT
GGGAGACAACTTGGGTACAAGAACGCTGCAAAGAATATCACATTCCGTGTCCGTATAAGGTGACACAAACAAGCCGACCGGACCTGATCCTCTTCCTGAT
GAAATACCTCATGGCGCTGGTGGTGGGGATCCCGTCTGTGTTCTGGGTCGGGAGCAAGAAGACCTGTTTCGAATGGGCCAGCTTCTTCCACGGCCGCAAG
AAAAAAAGCCGGCGTGAACGAGAGTCGCCAAGTGCTCCAAGAGCCTGATTTCGCCCAGTCGCTCCTCCGGGATCCCAATACCCCCATCGTCAGGAAATCT
CGAGGGACGTCCACTCAGGGAACCTCTACCCACGCTTCCTCCACTCAGCTGGCCATGTTAGACGACCAGAGGAGCAAGGCGGGCAGCGTCCACAGTAAAG
TGAGCAGCTATCATGGCAGCCTGCACCGCTCGCGGGACGGACGGTACACCCCGTGTAGCTACAGAGGGATGGAGGAGCGCCTCCCCCACGGCAGCCTGTC
CCACCTTACTGACCACTCGCGGCACAGCAGCACTCACAGACTCAACGAGCAGTCTCGCCAGGGCAGCATTAGAGACCTGAGCAATCCCCTGGCTCACATT
TCCCACGGAACCAGCATGAATCGGGTCATCGAGGAGGACGGAGCCAGCGCCTGATGGGACGGAGTGTAGAGCTTTAGAAGAGCCGATACTTAATGTTGGG
CTTATCTGGTTGGACGGTTCCAGCCCTTGATGACTGGGATCCCAGAATATGAACATTCTACTGAGTTTATAACCCAGCACCCTATTCTCATGGGTGGGGT
GAAACGACCCAGCACCCTATTCTCATGGCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGA
CCCAGCACCCTATTCTCATGGCCCAGGAATACGGCTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGACCCAGC
ACCCTATTCTCATGGCCCAGGAATACGGCTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGCTGGGTGAAACGACCCAGCACCCTA
TTCTCATGGCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCTATTCTCAT
GGCCCAGGAATACGGCTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCCATTCTCATGGCCCA
GGAATACGGCTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGCTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATA
CGGTTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTG
GGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGTTGGGTGAAACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGCTGGGTGAA
ACGACCCAGCACCCTATTCTCATGGCCCAGGAATACGGCTGGGGGAAAGAGCAGGGCCAACATACTGAAGCCCTCAGCCCCGGTTCCCACAATGGATTTA
TTTAGTGCTTGGTCCCCATTTCCCATTTCTAAATACACAGAGCAACTCTGTGATCTACAGAATCTACTTTCATTCGCTCTCCAGAGCAGGTACGGCCAAT
CGGCTTCCTCCCAGCTGCCGTCAAACTACAACTCCCAGCATTCCTCGACTGGGGTTTAGTTCAATAGCAGCAGAAGGAACATATTTTAGAAATGCCCCCT
GTGCGTATATACTGTACTCTCCCCCCCGGAGGGGGCGGGGCAAGCCTAAAAGCCTCTCACATTCAATTAGCGTGAGTCTTGTAAGCTCTTAACTAGCATT
GATCCTGCACTTTCCCCTCTCATTCCCTGGAGCCAAGGAGGGATAAAAGGCCAATTTCTTCCTGAAGAACAAATAATCTCGGTTTAGAGGCTACGGATGT
CCCAGTGTGGCCTGTAATATGGATCCGGGGACCAGCAGCAAATACAGGGGCACCAATATTGTCCATAGAAGCTTTTCTTCATCTTTAGACCCCCCCAACC
ATTCTATTCCGTTGCCATAGGAACTGGCAGCCCAAAGACTTTGCCCGGGAATTGGTCAGAGGACCAGGTCTCTAGCAACACTGTATTATTATATGGACTG
GAATGTAACGTTTTCAATAGCAGCGGCCGATAATTCCTATGGAATAGCCCTGCTTGGTAAATATGACCTGATGATGGTTCTGTACAGGAGTAAATGTGTA
GTAAATAGTCTGACAGCACACTGGTTTGTATATAAGGAATTATACACTGGATATTATACCCACAAATATGTACTACCCCGACTGCTGGCAACTTACTGTA
TTGTACTGGGGGTTTCATTCACTCACTGAGCACAGGCAGAAGGGAATGAGGCTCCGTGGCCTAATAATGGGCTCTTCCGCTTACTTGGAGGCTTTGGGCT
TATTTTATTCAGCCTCCTAAGGGCCCGTGGGAAATCCAACAGATTAATTCTCCCATCCAGTGCACTGTATATTGTAGCATTGGGCTACAATTCCCCCTAA
GCAGACAGTGGGCAGGGTTCAGTCCACTAGCAGGAAGCAGCATTGAATGATTTGTTTCATATATATATAATATATAGCTTTGAAGAGGTTACTGTTCATA
GAGGAACTGTCCAACTGGATTGCTGTATTTTTGTTATTGTCAGGGTCAGACTGGGCCGGCAGGACTCAAGCCCTAGTGGGCCCCCGCCCGTTCCTCCCAC
CCCCACTATATGTGCCAGCACCCCCCCCAGCCCGAAACGATTTGTGGGGGGGGTGGTACACGGCGGGGGTGGAAGGGGGAACAGGGACTCATGACTGAGG
TGGGAGGCCCCCAAGCGGCAGCCCCGGTGGGCCCAGTCCAACACTGGCTATTGCCCTTCCTACTGTAGTTCTGCAACATCCTAAGGACTGTAATTTGCCT
GATGCGGAGAGTGATATTCTGAGACAATTTGCAATTGGTCTTCATTTCTTTATTTGTTAATGTGAACTATTTACTTTCTGTTCAGCAGCACTTTTTGGAA
ATTCAGCACATTTTCTGGTTGCTAGGCTCTAAATTACCTTAGCAACCGGTGGTTTGAATGAGGCACTGGAACATGAACAAGAGAGGGCCTGAATAGAAAG
ATAAGTAATAAAGTAACAATATAATTGTAGCCACAGGGAGCAGATGTTTTTGGCTGCCGGGGTCAGCGACCCCCATTTAAAGTGTCAGGAGATGAAAGCA
AGTAGCTCAAAAACGATTCAAAATAAAAAATGAAGACCAATTAATAATGTGCTTAGAATTGGCTGTTCTAGAACACTCTGAAAATTAACTCAAAGGTCAA
CTTACCCCTAGGAAATGTGTATTTTACATTTTCCAATTCTAGTTTCTTATTAAGGAGCACCGTCCCTTTAACCCACCGTCCCTTTAACCCACTATTAACT
ACTGAATGTCTGTGCTGCTCTGTAGCCCATTGAGCCCCCCCCCCCGTGTTGCTGTTACATTAGAGGGGGGTCTGTACTTTATTTTGAAACACTGGAACAA
TTGAACATATTGACTTGTTTACATTGCGTTGATTTTGTATTTGCTTTATTTTTTACTGCGTCTTCTTTGTATTTTGTCCCCCATAGTGAAAGGGGCAGTT
ACAGAGCAGCACATACATGTACGGAACATACACGTGTATATTCATGGCTTCTGTATGGAGCTGCTTTACACTGGGAATCATACGGCAGCTCCATGCAGTT
TGTGACTGTGCCCCGGAGGCTCGGCGGCCATTTTGCCCGGCGGTGCATAAAGGGATTTGTACTGTAACGCAGTAAGGCAGCCTCTCTGTCTGTACAATAA
TAACGCCTTGGGTAGCACTTTACTGTAACAATGAGTGGAAAGAGAGGAGTAGATCAGTAGAAGGACATTTCATTGTAGAGAGAGGCTGAACTTGGCTTGT
TTTTTTTAATAAAATGGAAAATATATGAAATTAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX806394 [+]tccgcggccggtgcgtgtggtgctcccattgctccgtgtgagaggggaaacctgtcagccataaggggaggaaggagcgaacggttattgggcttttgga
ccttaaaatggagacagttgtttgggagtacggttaaaagaacgggcaacagagcttacattccatccttggataccatatagcaacaagatgaagtccg
ttgtagcgaagaggaagacagaagaaatatgtttattcctaatgtctgttctcctgcacgggggttactgaaatttccgtagtcaatgaaacttggaaac
cttcggacgtaggacattggcacttaaagggcacatgtggctttgcattctcccatcatggctgcctacgtgatttcctgtctttggttatcggtggtac
ttgcccagaaaagcatggggcacagtctgtttgcctgtgagccaatcacactaaggatgtgtcaggaccttccttataattccaccttcatgccgaacct
tctgaatcattacgaccaacaaactgcggctctggcaatggagccttttcaccccatggtaaacctggaatgttcccgggatttccgcccattcctgtgc
gctctttatgcgccggtgtgcatggaatacggacgcgtgacgctgccctgccgcaagttatgccagagagcctataatgagtgcttcaaactcatggaga
tgtttggtgtgccgtggccagaagagatggaatgtagccggtttccagactgcgatgagccatacccccgaatggtggatatcagcctgtccggagatgt
gac
3' EST: CX806393 [+]aatttcatatattttccattttattaaaaaaaacaagccaagttcagcctctctctacaatgaaatgtccttctactgatctactcctctctttccactc
attgttacagtaaagtgctacccaaggcgttattattgtacagacagagaggctgccttactgcgttacagtacaaatccctttatgcaccgccgggcaa
aatggccgccgagcctccggggcacagtcacaaactgcatggagctgccgtatgattcccagtgtaaagcagctccatacagaagccatgaatatacacg
tgtatgttccgtacatgtatgtgctgctctgtaactgcccctttcactatgggggacaaaatacaaagaagacgcagtaaaaaataaagcaaatacaaaa
tcaacgcaatgtaaacaagtcaatatgttcaattgttccagtgtttcaaaataaagtacagacccccctctaatgtaacagcaacacgggggggggggct
caatgggctacagagcagcacagacattcagtagttaatagtgggttaaagggacggtgggttaaagggacggtgctccttaataagaaactagaattgg
aaaatgtaaaatacacatttcctaggggtaagttgacctttgagttaattttcagagtgttctagaacagccaattctaagcacattattaattgggctt
cattttttattttgaatcgtttttgagctacttgctttcat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 0.5-1.5 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn3 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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