XB-CLONE-3651784
Clone Name: IMAGE:7653423
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Gene:
clstn3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC129013 [+]
GGGCGCACAGCATCCCTCACTGCAGAGCATCCAGTGGCTACTGAGCATCTCCCTCAGCAGAGCATCCTGTGGGCACGGAGCAGGAGACAGATTCACTGCT
GCATGTAGATACCCCAAGACAGATCAGTGAAAACGTGAGACTGCTTCCAGGAAGATGCAGCACCGAGGCCTTCATCTGTCTGCGCTTCTCCTCAGCGCTC
TGCTGCACTGTGCTGCTTCTAACAAGGGTAACAAGCACAAGCCATGGATAGAGGCGCAGTACCAAGGGATAATCATGGAGAATGACCACACTGTGTTGCT
TAACCCTCCCCTGTTTGCTCTGGACAAAGATGCCCCACTGAGATATGCAGGTGAGATTTGTGGATTCCGGATCCATGGCTCTGGTACCCCCTTTGAAGCT
GTGGTTCTAGATCGGACAACGGGAGAAGGGCTGATAAGAGCCAAGGGCCCTGTAGACTGCGAGGCCCAGAGGGAGCACACCTTCACTATCCAAGCACATG
ACTGCGGAGAGGGGCCTGATGGGGTGAACAGCAAAAAATCTCACAAGGCCACAGTTCACGTGCGCGTGAATGATGTCAATGAATTTGCCCCGGTGTTTGG
AGAACGGGTTTTCAAAGCCTCAGTCACTGAGGGGCGAATGTATGAGCGGGTTCTACGGGTGCAAGCTTGGGACCAGGACTGTTCTCCCCAGTACAGCCAA
GTCTGCTTCTACCAGATACTCACTCCCAATGTGCCCTTCACCATTGACAATGATGGCTACATAAAGAACACACAGCCACTGCAGGCCAGCGGGGAGAAGT
TGATCCGATTTACAGTCACGGCCTACGACTGCGGCAAGAAGAGAGCTGTTGAGGACGCAGAAGTTGAAATACAAGTCAAACCAACTTGCAAACCGAGCTG
GAAAGGCTGGAACAAACGCATTGAGTATGTGCCTGGTTCCGGCAGCTTGGCCTTGTTTCCCAACATTCATTTGGAGACGTGCGATGAGCCAGTGGGGAAC
ATTCAGGCCACTGTGGAAATCCAGACAAGCCACATTGCCAAGGGCTGTGACCGAGACAATTACTCCGAGAAGGCCCTGCGCAAGCTTTGCGGAGCATCCC
CAGGGGACGTGGACCTTATGCCACCGCCCAGTCCCATCCATAACTGGACTCACAGTCTCCCAGTTCAGGACACACAAGACAGCAGCCTTGTCTTTTGGTT
TAATGGATCCCAATCCGTGGAAGTTGCCCCCAGTCACCTACCAGCAGGGGGCAGAACTGCGGACCACCTGACTCTCTCGTTCTGGTTGAAGCACGCCGGG
GGGAGTGGGGGACGGGCGGGGAAGGAAGAAGAGGTGCTTCTGTGTAACACGGTGCAGAATGGAGATGGGAGCTTCTCTCATTATACACTGGCTGTACATG
GCTGCCGGATCTCCTTTCTCTATTGGTCTCTTCTGGAAAGTGCCCGTCCTGTTAAGTTCCTTTGGAAACTGGAGCAGGTGTGTGACGTAGAATGGCACCA
TTACGCTCTCAGTCTGGAGTTCCCGACAGTCTCTCTGTATGTGGATGGAGTGACCTACGACCCTGCGCTTATCCACGACAACGGTGCTCTCCCTCCTCCC
AGGCGCCAGGCTCGGCTAATGATCGGGGGGTGCTGGGCAGAGGAGAAATTGTCCGTTAAAGTGGACGACAACGAGACAACGAGAGTGACAGGCACATTCC
CAGGGCGCTTCCTGAGGGGCTACATGTCGGGCCTAACCCTGAGGCTGGGGCCTGTGGATTCCCGGGAGGTGATCGAGTGCCTGTACTCCTGTAAGGAGGG
ATTGCAGTATACGGATTTCGACAGTCTGGGAAAAGGAATGAAGGTTCACGTCAACCCAGCCCAGTCCCAGCTGTCCCTGGAAGGGGACGATGCCCATTCG
TTTAGCCGCGCGCTACAGCACGTAGAGTACCTCAACTCATTGCAGTTTCCCACCCCAGGAGTGAGGCCTCTCAAACTGCAGACTATAGTCAAGTGCCCCA
GTGATGAAGGCTGTCTCTCAGTGCCAGATTTGGAAGGATACCTTGTAGTTTTGCAGCCCGATGCTCCACAGGTTCAACTTTCTGGCTCGCCTCGCTCTGC
ACACCCCGTCTCTGACTTCCAGGGGCCAGTGGGGGTCCCCATATTCCCAGACCTGAAGATTACCTGCTCAGTGTCTCATCCCATCAGCCCAAACAAGCAG
GAGAGAGCTCCCAGGGCTGATATGCAGTCTCCTGATGGGATTGAGTGCTCTCTAGAAAAGTGTGAGGTTGGCGTGGTAGGAGAGGACCTGAACGTGGAGT
ATGAAAGTCTGTCTGTGGATGTCTCTTCTACATGGCAACGTGGCCTTGAGATGGAGAACAGCTCCAGGAGCCTAAAGATCACAGGGGTGCAGAGTGTGGC
TGTGTATGAGGATATCCTGCGCGGCTTGACTTACCGAACAGTGGCTAGAGCAGCTTTATTTGCCCGCAAATTCCATGTGTCCTGCAGCGAAATGAATGGG
AGGTATATCAGCAACCAGCTATATACAGAGGTTGATGTGTTACATGGAATCCACGCTGGGTCCCCCAATCATGTTCTCTCCGCCCAGCAATTTGTCCATA
GTGCACACCAGGCCCCTGCAGAGTTATCAGGCCACACGCTGGCCAATCCCAACCCCAGCTCGGTCTTCCCAGGCGTGGCCATGGTGATAATCATTGTGTG
TATTGGCTTCTTGGCACTGATTGTCACACTGGGCTTGTCACGGATACACAGTGTGCGGCAAAGAGATGGCAATGAGGGGGCAGAGCTGGAGGCAGCGACT
CAGAGAGATACGTTCTGGGAAGACTCAGCCATGACGATTACTGTTAACCCAATGGAGTCCTTGCAGTCTCGTGGCTTGGTATCAGCACAGAAGGAGCCCG
TATCAGAGCGAAGGGAACCAAATGAGGAATCCGGTTGATTGGACCCACGAATGTTGTTTCTTCTCATCCACCCATCTGTGTACTTTTTTGCCTATGTGTG
ATTTCAGACTCTTTCCACTGGGGATGCGCCACACATCTCTCCATAGGATGAGTACTAGTCCCTGAAGATCCGTACTGTGGGGGAGAGAGCACAGTTCATC
TTCTGTGGGGTGTGTGCCACCTTGTTACTCTCCTTTCTTTGTGGGAGGGGCTCCTTGGGCCTCTGTCGTTGTGGGAGGAGCTCCTTGGGCCCATGAGTTT
CCCCCCATCAGCCTCTCACCTCTCTGTGGGTGTGTACCACCTTGTCGTTGTGGGAGGAACTCCTTGGGCCCATGAGTTCCCCAATGGCCTCTCACCTCTC
TGTGGGTGTGTGCCACCTTGTCATTGTGGGAGGAGCTCCTTGGGCCGCCCCTGTCATTGTGGGAGGAGCTCCTTGGGCCCATGAGTTTCCCCGACGGCCT
CTCGCCTCCCTGTGGAGCGGGCGCCACATTCCTCCAATCATTTCTGTGAGCTGTGCATCAAATCTGCCCCATCTCTTCATCCAACTTCCATCTCCAGTTA
CTTTGTGAGCGTCACGTCCATAGCTTGTGTGTTGGGAGGGAGGGGGGGGGGATACCAACATCCTGATACTTTTCTAAGTCCGTTCTGCCGACTTTTATTT
CTTTCCTTTGAGCGTGTGAATGTGTATGCGGCGTTTGGCTGGCCGTAGCGCTCTGCCTCATTCTGGGCGCCAACAACATCATTTCTCAGGGGGAAAAAAA
TCTATCACAAAGTCTCATTTCCTGATGTCTCTGTGCGACTGTCTTGAGTGTGCCATCTTTACACATCCAGCCCCTGAGAGACTGCAACCTCTCAGCTGTC
TCTTTCTCGCGCTCTCTCACCCAATAGATTTCACCGTTGCTGGAGATCCGTTTCTGTGTTATTACCGGTTCTTTAAATCCTCCTCCAGCACAGGATGAAA
CTTCACAGACTGACTTGGGCCACGTGCCCAACACAATAACGCTGAGCTAACACTGTACAAACAGGAAGCCATTGCAGTGTTACCCTTGTCCCTCACACTT
TGTTCCTGTCTTTACTGTTGTGTTCCTCAAAATCTCTCCTCCCCCTTCCCCCCCTCGCATACCCAACTGCCTCTATACCTGGGGTATTTGCTGGAGGGGG
TGGTTTCACGACAATGAATGAAATAACACACCAATTTCTGGTAAATATAAATATATATAAAACACTATATATATAAAGAAATATATATAGAAATATAAAA
AAAAAAAAAAAAA
5' EST: CX822114 [+]agcatccctcactgcagagcatccagtggctactgagcatctccctcagcagagcatcctgtgggcacggagcaggagacagattcactgctgcatgtag
ataccccaagacagatcagtgaaaacgtgagactgcttccaggaagatgcagcaccgaggccttcatctgtctgcgcttctcctcagcgctctgctgcac
tgtgctgcttctaacaagggtaacaagcacaagccatggatagaggcgcagtaccaagggataatcatggagaatgaccacactgtgttgcttaaccctc
ccctgtttgctctggacaaagatgccccactgagatatgcaggtgagatttgtggattccggatccatggctctggtaccccctttgaagctgtggttct
agatcggacaacgggagaagggctgataagagccaagggccctgtagactgcgaggcccagagggagcacaccttcactatccaagcacatgactgcgga
gaggggcctgatggggtgaacagcaaaaaatctcacaaggccacagttcacgtgcgcgtgaatgatgtcaatgaatttgccccggtgtttggagaacggg
ttttcaaagcctcagtcactgaggggcgaatgtatgagcgggttctacgggtgcaagcttgggaccaggactgttctccccagtacagccaagtctgctt
ctaccagatactcactcccaatgtgcccttcaccattgacaatgatggctacataaagaacacacagccactgca
3' EST: CX822113 [+]atatttctatatatatttctttatatatatagtgttttatatatatttatatttaccagaaattggtgtgttatttcattcattgtcgtgaaaccacccc
ctccagcaaataccccaggtatagaggcagttgggtatgcgagggggggaagggggaggagagattttgaggaacacaacagtaaagacaggaacaaagt
gtgagggacaagggtaacactgcaatggcttcctgtttgtacagtgttagctcagcgttattgtgttgggcacgtggcccaagtcagtctgtgaagtttc
atcctgtgctggaggaggatttaaagaaccggtaataacacagaaacggatctccagcaacggtgaaatctattgggtgagagagcgcgagaaagagaca
gctgagaggttgcagtctctcaggggctggatgtgtaaagatggcacactcaagacagtcgcacagagacatcaggaaatgagactttgtgatagatttt
tttccccctgagaaatgatgttgttggcgcccagaatgaggcagagcgctacggccagccaaacgccgcatacacattcacacgctcaaaggaaagaaat
aaaagtcggcagaacggacttagaaaagtatcaggatgttggtatcccccccccctccctcccaa
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: