XB-CLONE-3650787
Clone Name: IMAGE:7653814
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Gene:
osbpl1a
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC153360 [+]
GAGGGCTAGCCATGGGAGACGTGTACTGCCGAAGCTGCTTCATACATTATTGCCTGTGGAAACATGATGCACAGCTGGCCGGAGGAGACATCACCAACAA
ATTAAAGTAACATCACTTCCCAGGAGAAAGGTAAGGAACTTAAAACTAGAACACGAGCAAGAGAAAAATAAGATCTTATCCGAGGCCTTGCAAACATTGG
CTACAGAGCACCACGAGTTGGAAAAGTCACTGGTTAAAGGAACTCCTCCCCTTCATACAATTCTTAGTGAGGATGAGTTCTATGACGCTGTTTCAGATTC
CGACTCTGATCATTCCTTAAGCGGATTTGAAACTGTTACAAGTTCATCTCCAGACGGGAGTTTAAATTTGAGCTACAATCAGCAAGTCAGCATGTCTGAA
GAAAAGAAACATGGGGGAGGAGGAGGTGGCTCCACATCTAATGGCATTAAAAAGCACAGAACAAGCTTGCCCTCCCCAATGTTTTCCCGAAATGATTTCA
GTATTTGGAGTATTTTAAGAAAATGCATTGGAATGGAATTGTCGAAAATCACAATGCCTGTTATTTTTAATGAGCCACTTAGCTTTCTACAACGCCTCTC
TGAATATATGGAACATACGTATCTCATCCACAAGGCAAACCAGTGCTCTGATACAGTGGAGCGAATGCAGTGCGTAGCTGCTTTTGCAGTGTCAGCGGTT
GCTTCACAGTGGGAGCGAACTGGCAAACCATTCAATCCACTTCTTGGCGAAACATATGAATTAGTTCGGGAGGATCTTGGATTCAGATTAATATCAGAAC
AAGTTAGCCACCACCCTCCTGTCAGTGCGTTCCATGCAGAGGGAATAAACAATGACTTTGTGTTTCACGGGTCCATTTACCCTAAACTCAAGTTCTGGGG
TAAGAGTGTGGAAGCTGAACCAAAAGGAATTATTACTCTGGAGCTTCTCAAACATAAGGAAGCCTACACATGGACAAATCCTACTTGCTGTGTACATAAT
ATAATAGTGGGCAAACTCTGGATAGAGCAGTATGGCAATATGGAGATAACCAATCACAAAACTGGGGAAAAGTGCATTTTAAACTTTAAGCCATCAAGGC
TTTTCGATAAAGAACTGCACAAAGTGGAAGGATACATTCAAGATAAAAGCAAAAAGAAGCTGTGTTCCCTGTATGGGAAGTGGACAGAATGCTTGTATAC
CACAGATCCAGCTACATTTGATGCATTTAAAAAGAATGACAAGAAAGAGGTAGAAGAAAAACAGGTAAAGGTAAAACAGGCTGGAAATGCAGAGGAACCT
GATGAAATGCCATTACCTGATTCAGAAAGTGTACAGGTTATTCCAGGGAGCACACAGTTGTGGAGAATAGCACCTAGACCTCCTAATTCTGCACAGATGT
ACAATTTTACCAACTTTGCACTAGCCTTAAACGAGTTTGATAAGGATATGGAAGGTACAATTCCTAAAACAGACTGTCGACTACGTCCAGATATAAGAGC
TATGGAAAATGGAGAAATTGACTCAGCCAGTGAGGAAAAGAAAAGATTAGAAGAGAAGCAAAGAGCTGCACGGAAGAGTCGTTCCAAAACTGATGAGGAA
TGGAAAACCAGATGGTTCCGACAAGGTCCCAACCCCTACAATGGTTCACAGGATTGGCTTTACTCTGGAAACTACTGGGACAGAAACTACTCCAACCTGC
CAGATATTTATTGAAACTGGCTCATATGAGTGACAGACATATTTCCAACTGTATCCAATGTAGATTTGTGTGTTTGACTCTTCAAGAAATAAAAACAAAA
TGCACCTTTTATAGCACTCTAATGCTAGCACTTAATTTAAGCATTCCAACTGGATTAGTGGACATTTTTGTAAGAAAGAGACAGCCAAGCAATATTTCAG
GGAGCAGTGTTCAACATTAAAGTCACGGTGGGAGTTTAAGCTTGAACTTGCTGGCAGCTATCTATTTAAAGCAGTTATCTGAAATTGTATGATCGAGAGA
AGGGGCTCCACCCTTTGAAATATTGGGTGCTTTTCTCTCTGGTTATACTGAAGGCTTGATATACTAAGCACAAATAAGCACTGAATGTTTTAGACTTAAT
ATGCAGGATGTGGGTTTGTTTCATTGTTACTCATTATCCAGTTGCTTTCAGTAGTCCTATGGCTGCTTATGGTATAACTGGCATGAGGGGCAGCACTTTC
TTTGCATTCTTTATGAAGATCATTGCTTATGAAAGAAAGGAAGCAGAATCCATTGTTTTATCAGGTCAGCCTTGTTTTTAATCCTTTAATTATGGGGTTT
ACATTTCTATTTTGTCTTAGAATTCTATTTATCATATCAAAGTGTATTTCTAGAGATAAAATCTTCTATATATTATATTAAACAGTAGATGTTATTAATA
TGTAGGACAAAGTTGGCAAAACTTGCTGACAGAAACACATATTCCAAAAGATATGTACTCTGTGTTGTCACTTTCCATGTAGTAGCTAATAATCTAATAT
GTGGTGCTCACGTGTAATAAGATAAAGCTGTTGGATTGGAATCCTCTGCTTAAATCTATATCACAAGAATTCATTATTTAAGGACCACAGAGAAGCCATA
TAGTGACAACAAACTATGTGCAGCGCTGCAGGGATACCATACTAACTCCCCTCTCTGTGGGATGTTCCACTTGTAGCAAAACTCAATGACGGTGCAGCAG
CATAGACTGTGAGCATCCGACTCCTGCACATCTCTCTGCCAGGCACACACAGTATCTTGTAACGGATATACATAATGTCTTGCACTTGTGTATATTCTTG
TATCCCTCTTACTCGAGGTGCCTGTTTTGGAAGAATTTGTAGAGTCCATTGGCACAAAGCACTGTGGGAAGAGCAGTAGAATTAGGAACTCTAATAATTG
TTTAGCATATAATAAATAAAATAACCTTAATTTATCAAGTGCTGCGAAATAAAAGCATTTAAAAGCGCTGAAAAAAAAAAAAAAAAA
5' EST: CX823238 [+]ggctagccatgggagacgtgtactgccgaagctgcttcatacattattgcctgtggaaacatgatgcacagctggccggaggagacatcaccaacaaatt
aaagtaacatcacttcccaggagaaaggtaaggaacttaaaactagaacacgagcaagagaaaaataagatcttatccgaggccttgcaaacattggcta
cagagcaccacgagttggaaaagtcactggttaaaggaactcctccccttcatacaattcttagtgaggatgagttctatgacgctgtttcagattccga
ctctgatcattccttaagcggatttgaaactgttacaagttcatctccagacgggagtttaaatttgagctacaatcagcaagtcagcatgtctgaagaa
aagaaacatgggggaggaggaggtggctccacatctaatggcattaaaaagcacagaacaagcttgccctccccaatgttttcccgaaatgatttcagta
tttggagtattttaagaaaatgcattggaatggaattgtcgaaaatcacaatgcctgttatttttaatgagccacttagctttctacaacgcctctctga
atatatggaacatacgtatctcatccacaaggcaaaccagtgctctgatacagtggagcgaatgcagtgcgtagctgcttttgcagtgtcagcggttgct
tcacagtgggagcgaactggcaaaccattcaatccacttcttggcgaaacatatgaattagttcgggaggatcttggattcagattaatatcagaacaag
ttag
3' EST: CX823237 [+]cagcgcttttaaatgcttttatttcgcagcacttgataaattaaggttattttatttattatatgctaaacaattattagagttcctaattctactgctc
ttcccacagtgctttgtgccaatggactctacaaattcttccaaaacaggcacctcgagtaagagggatacaagaatatacacaagtgcaagacattatg
tatatccgttacaagatactgtgtgtgcctggcagagagatgtgcaggagtcggatgctcacagtctatgctgctgcaccgtcattgagttttgctacaa
gtggaacatcccacagagaggggagttagtatggtatccctgcagcgctgcacatagtttgttgtcactatatggcttctctgtggtccttaaataatga
attcttgtgatatagatttaagcagaggattccaatccaacagctttatcttattacacgtgagcaccacatattagattattagctactacatggaaag
tgacaacacagagtacatatcttttggaatatgtgtttctgtcagcaagttttgccaactttgtcctacatattaataacatctactgtttaatataata
tatagaagattttatctctagaaatacactttgatatgataaatagaattctaagacaaaatagaaatgtaaaccccataattaaaggattaaaaacaag
gctgacctgataaaacaatggattctgcttcctttctttcataagcaatgatcttcataaagaatgcaaagaaagtgctgcccctcatg
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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