XB-CLONE-3650287
Clone Name: IMAGE:7654174
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Gene:
fastkd5
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121847
[+]
CAGGGAGACGGTGCGCAGCTCATGCCCTTGTCCTGCGGTTCCTTCATGTCGGCTTGTGGGCTCCCACCTCCATAGGCTCCCTACCTGGTAACTGGATGCT
TTTGTGATGCTGGATCCCGTGACCCATGGCCACTGTGATATGCCGAAGATTGCGAGGAGGGGTGTACCGAACCAGCACATTTTCAGCTGCGGTCAAGCTG
GCGCACAAGAAGCTTCCCAATGATCACGGAAACGCTTACACTTTGCACATCTTGGAGAATGAACACGTTGAACCCGACAGAAATGGTTTAAACCCAATTC
TGTACAACCCAGGGGCTTATGCAGGGCTCAAAAAATATAACCGCAGTGCGACAGAGAGCTATGAGGTTAATACATTGCACAGTGACTACACTAGGAGTAA
TGCCAAGCAGATTCAAAATACGTACAGTATTATGTCTTCCCGAAGCTTATCTAGTGCAAAAAATAATATATTAGACTTTGCATTTAGCAGCCCAGTTACC
TCACAGGCTCGACTGCCAGATAATACAAAGCCACAGTTGAAAAATGAGGCTGAGCCTAAGCTTGATGCCTATGAGGCTGAAATCTATAGCACCAAGGAAG
ATCCAAGAAGGTTTCAGTAGGACAGGGACAGGCCGGAGTACAAATCCCTGTGCTACAGCAACTCCACCCAAAGTGTATGCACATCTGTAGAGGAAGGGCA
GCACCTTCTGAAACAAGTTACCTTTCTGAAGAGCAGTCTGACTCCCGGAGTCATCGCTGACTATTTTGATAAGCTCGGCCATTTACCTGACGATCAAATG
GAATCCGTACGGGCAGATACTAAGTTTGCCATGCTCTGCCGCTACAGCATTGAAAACCTCCAGCAGTACAGTCACGCAGAGCTGATCGGTATTCTGAAGG
CATTTGTACGTCTCGAGATTCCCGCGACCCACTCCATGTTCAGTGTCTACGAGGTGGAGTTCTGTCGTAGGGTTTGGAACATGAGCACAAACGACTTGCT
GTTAGTAGCAGATATGTGGAGGTACTTGGGTCGTAGCGTACCTCGTTATTTAGAGATCCTATACAGTTATATGGAACTGCGTTGGAAGGATCTTAATTTG
CCCCAGCTGATTCAGTTGATCTATATCATTGGGGAAGGGAGGAAGGCACCTCGGGAGCTGATGCAGAAACTTGAATCGATGGTGCTGAGACACCTAGATT
CGCTCAACCTGGAAGAAATCGGTGCTGTTTGCTTAGGCTTCTTTAAGTCTCATAATGGATTGTCCGAGCATCTCATGAGAAAAATCGGGGACAAAGTTTC
TGACGGAATGGACGACATTAGCAATTATGCTTTAGTTAATGTGCTTAAAATGTTCCGCTTCACCCACGTAGATCATTTAGTCTTCCTGAAACGACTGGGA
CAGATTGCACCGGGCAGAATCCCCAGTATGGGTTCCCAAGGGATCATGCACATTGCTCTGTCTTGCGCTGCCTTGCATTATCTAGATGAAAATGTCATGA
ATGCCGTAGCGGCCACAATTCCAGACAGGGTTGCTTACTGCAGAAGCAAAGACCTTGCCAAGCTTTTATGGTCCTTCGGTGCTTTAAATTACCAGCCTCC
GAATGCCGATCAGTTTTATGCCACCCTCACTTCTCAGATTCGGAACAAACTGGGGGAGTTTGAGAAGTTTCCCGAACATTTCCTCACCTGTTTGTTGGGA
CTGGTATTCGCTAAATACTATCCGTTGGATCTGATCGAGTTTGCCTTAAGCGAGAAGTTTGTTAAATTAGCTACCAAGGAAAGCTTGTTTGAGCTTAAAA
AGGATCTTTTCACTTTAGACGGAAGCGTGGAAATCGAATGTCCCGAGTACACGGGTAATCACCTATCGATGGAGCTTCGACAAGAGGTGACAGAAATGCT
CCAGAGTTTCTCAAGGCAGGATATTTGCATCAAACCTGAGGTTCTTGAAGCTGCTACCCTAATAGAATCCATGTTGGGGGGACCACAGTACGTGAAAAAT
CACATGATTTTGCCTCACACTAGATCAAATGACTTGGAGGTCCATTTGGACGTCGGCGAGAAGCCCATTCCTATAAATGTGGACACGGTGGGATCTCCTT
CTGTTAGCTCTGAACTGAAACCTATGGGCATCCAAATAACGGAGGACCTTCTGGATCAACTGTTGGACAGCAATCGGAAAACTGTTCTGCACAAAGATGT
AGAAAAGCCAAAGCTTGAGACTGGCCAACGCAGGGTGGCTTCATCTGTTCCAAAGGATTACACAAAACTACTCAATCCTGATTTCTCCAGTGGTGTCCCC
ATTACCGACAACCTAATATCTATGCTGGCAATGTCAAGGGCTTTACCTGAAAAACCTTTGTGCAAACCGAAAGCACGTGCAGACGCTTTCAAGCTGGCTA
TCCAAGTATCCAACCGGAATCACTACTGCTATGCCTCTAGGCATCTCCTGGGACTCCATAACTTGAAGAGAAGGCAGCTCCAAAAGTTGGGATATGTAGT
GGTGGAGCTTCCATACTGGGAATGGTTTCCTCTGCTGAAACGTACACGTTCCGAGAAACTCGCTTACTTGCACCAAAAGATATTTAGCTCCGTGGACTAC
CTCCGGGGGTAACGTGCTTCTTGGTGGACCGGCATCGTTTCTCTCAAGTTTGGGCAATGCGAGCGGCAGTAGGAGAGGTCGATGGTGGCAGTTGAATAAA
TTGCAATTAGTCTATCGGCACTTCAGCTTCCTTTTGTAATTACCAGCATTTAATCCAGTTGTATATACAGTACCAGTTTGGGGGTCTGGGTACAAAAGCA
GGTCACGTTTAATAATCATATTAATCATAATTTATGTTTCTTAATGCCTATATTTTTTTAAAGTGGTGTTGCACAACCAGACTGCCCTATTCTGAAATCT
TTATTCTGTATAATAAACAGTTTAATAAAATAAAAGTCAGTGGTTTGAATGCCACGGGAGTGACTCTAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX823771
[+]cagggagacggtgcgcagctcatgcccttgtcctgcggttccttcatgtcggcttgtgggctcccacctccataggctccctacctggtaactggatgct
tttgtgatgctggatcccgtgacccatggccactgtgatatgccgaagattgcgaggaggggtgtaccgaaccagcacattttcagctgcggtcaagctg
gcgcacaagaagcttcccaatgatcacggaaacgcttacactttgcacatcttggagaatgaacacgttgaacccgacagaaatggtttaaacccaattc
tgtacaacccaggggcttatgcagggctcaaaaaatataaccgcagtgcgacagagagctatgaggttaatacattgcacagtgactacactaggagtaa
tgccaagcagattcaaaatacgtacagtattatgtcttcccgaagcttatctagtgcaaaaaataatatattagactttgcatttagcagcccagttacc
tcacaggctcgactgccagataatacaaagccacagttgaaaaatgaggctgagcctaagcttgatgcctatgaggctgaaatctatagcaccaaggaag
atccaagaaggtttcagtaggacagggacaggccggagtacaaatccctgtgctacagcaactccacccaaagtgtatgcacatctgtagaggaagggca
gcaccttctgaaacaagttacctttctgaagagcagtctgactcccggagtcatcgctgactattttgataagctcggccatttacctgacgatcaaaat
ggatccgtacgggcagatactaagtttgccatgctctgccgctacagcattgaaaacctcagcagtacagt
3' EST: CX823770
[+]agagtcactcccgtggcattcaaaccactgacttttattttattaaactgtttattatacagaataaagatttcagaatagggcagtctggttgtgcaac
accactttaaaaaaatataggcattaagaaacataaattatgattaatatgattattaaacgtgacctgcttttgtacccagacccccaaactggtactg
tatatacaactggattaaatgctggtaattacaaaaggaagctgaagtgccgatagactaattgcaatttattcaactgccaccatcgacctctcctact
gccgctcgcattgcccaaacttgagagaaacgatgccggtccaccaagaagcacgttacccccggaggtagtccacggagctaaatatcttttggtgcaa
gtaagcgagtttctcggaacgtgtacgtttcagcagaggaaaccattcccagtatggaagctccaccactacatatcccaacttttggagctgccttctc
ttcaagttatggagtcccaggagatgcctagaggcatagcagtagtgattccggttggatacttggatagccagcttgaaagcgtctgcacgtgctttcg
gtttgcacaaaggtttttcaggtaaagcccttgacattgccagcatagatattaggttgtcggtaatggggacaccactggagaaatcaggattgagtag
ttttgtgtaatcctttggaacagatgaagccaccctgcgttggccagtctcaagctttggcttttctacatctttgt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: