XB-CLONE-3649458
Clone Name: IMAGE:7654864
|
Gene:
adcyap1r1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX824630 [+]
ccgacatatagatggtaaaaaggattcaatggaatttcgatgtaaatcgatgaaaattggagatgactttgtctgagagatagttttgctgtatttctgg
atgactagatactagattcccatacctgtagtctataaatataatgtgggtgttctggtactatggtaagatagcctcagaccaacctgcagccccaagc
tgttttcggatgatggcagttatttcctgcaacagcttgagggatacaaggtggacaatctaaacatagtcacctccttcaatgtcttgagggaccaacc
tgtagccccaaactgttttcagatgatgtcagttatctcctccaatagcttgaggaatacaaggtggacaatctaaacatagtcatctcctccaatggct
tgaggaactaacctgtagccccaaactgtttttagatgatgccagttatctcctccaatagcttgaggaatacaaggtggacaatctaaacatagttgtc
tccttcaaaggcttgagggaccaacctgtagccccaagctgttttcagatgatggcagttatctcctctaacagcttgaggaataaaggtggacagtcta
aacatagtcatctcctccaatggcttgagggaccatcctgtagccccaagctgttttcagatgatgtcagttatctccccccacagcttgaggaatataa
ggtggacaatctaaacatagtcatctcccccaacagattgagggaccatcctgtag
3' EST: CX824629 [+]gaatgtcattattctttttactaaactcagaagtcggtttttgagaaagaaaatgtcggttatccgataacatgtacaaaaaaacgtagccacgagatta
gcatcacacataatatatagtacaataaaaacagccaaaaaaagtatttataacacataaaatattactggacgacaatcaaaatggctcagtaacacat
gttatggcacgatacgtatcacaatccctttcgttcccagtcggagcacaaatggcgaccggtactctttacattccccatttatatacattttctgcat
ttctgcttgagttgccttggtcccgtcacatgtacaaaaatacggcaatgggtatttgtaatgttttacattcaaggaacaaagagcatagaccatttat
cgcatcacccactgatcggcagatacttcgtaaaatgtcgatattttcaaataccgtgttaaattctcactaaaaataaactaaaatatatatatctata
gacatctccagtgttctttcttctgttcttctgtatctgaatttataactaggctattgttaaaatgacccttctgcattgtataaagtgtaacattgac
atcatcgcggttggacataagaaaatatgtactggctgtgactctgaataacaaggcaaagttatagaactgtcagaaaataggcatttccgtcttaatt
gtttatatttgtgaaaaaatattaattggcaatatagttttttttttctgtt
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: