XB-CLONE-3648280
Clone Name: IMAGE:7655532
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Gene:
entrep3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136030 [+]
CTGGAAACCAGTGATCCAACATCCTTCTCTTGTGTAGGGTCCTGCCCAACCAATGTAAAGGCTGGAAGCCTCTATAGACTGGGGCACAGTTATTGGTACT
GGATTTTTCTTCGGACCAGGACCTTTTTTCCAGGGCTGAGTTCTTGGTGCCCCTTCCTTGGGTGCAATTCCGTCCCCATTTTGCTGGGGGAGCTCTAATG
AGAGAATCTAGAGGCTGTTGGACGCCCTTGGGTTCTTCAGTCGGAATCTATTGACTTTTCTATCTTTGTTCCAAACGGACCTGAGTGTTTCCCTCAGCAG
TTCTGCCACAGGCTGGGGCATTATGGAGTAGGATGAAAGGTTGGTCTCACCCTCCTGTTCCCCGTAGCCAATAACGTAGGTTTGGTGGCCAGGACCCAGG
TCTGCAGAACTTCCCTGAGGAACCCTTCTGCTCCCAGACTGGGTCTCCTTACTGATGAGTTCTGTGGTTAATCTGATGCTTCTGGACCCAGTGTTCTTCT
CCGTCATGCCCTCTCCTACGGACTCCAGCTTCTCCCTCACTGGCCGGACCTCTCGCAGTCTGTCTCAGATCCGGCTCCACAGAACCTGGTCCCACGTCCT
ACTGGCCCTGGGGTTTGTGCAGCTCATTCTGGGGGTATTAATCGTGACGTTTAGCCTGGTGGCTGCCACTATTACCCCGTCCATGAAGATCAAGCACTCG
TGTCCTTCCTGGGCCGGATTCTCTCTGGCCGTATCAGGACTGATTGGCTGCATTTCCTGGAGGAGGCCCCTGACGCTGATGATCACGTTATTCACCCTGC
TGACGGCGCTGTGCGTTATGCTCAACCTGGCGGGATCCATCTTGTCCTGTCAGAATGCTCAGCTGGTCAAGTCCCTGGACAAGTGCAGTAAAGATAATTA
CTGGTGTGTTTGCTGCCACCCAATGTCCGAGGTGCAGATGCCAACGTCCTGCCGGCGAGATGTGGAGATTCTCACCATGTACCCCAATTCTGAATGTAAC
AGCATCCGCAACGCCCTGAAGGTAACGGGGGAGGGACGCTGGGGGTATTGGGGTATTGGGTGCCGTTATTTCTGACTGGGAACATCAGACGGGGTTAATG
CATTTATAGTTAATTATTTTTTGAGGGAGGGGTCTTGGTCAGTGTGTAAATCTGCTCCTAGTAGTTGATATGATGTCCAACCTGGTCACATGACCAAGAA
CTGAAGGCAAACTTGCCCCGGGGTTCCAGTGGGGCAGCTTTAGATGTATTATGGTTTCAGTAGGACTTACTCCTTACCCATTTCTTTTCTCCTTACTAAA
TCTCCACGTGATGCCTCCTCGGAGTTGCCTCCTCCCCTAACTCCACTGCTGCCTCCTGCTCCCAGGGCTCGCGCCTCGAAGTGCCACCTCGGGCAACTTG
GAGTGCATTTCACCCCAGGACACCCTCTTGCAGAGTATGATAGAGTGCGAGGAGTTTGTCCCACCGGTGCCCCCACCTCCCTATTACCCCCCAGAATACA
CCTGCAGCTCTGAGACGGATGCACAGAGCATCACTTATAATGGCTCAATGGACAGCCCAATCCCTCTCTACCCAACTGACTTCCCTCCCTCCTATGAGAG
CGTCATGGGAATCCGCGAGGACAGCCAGGCAACGTTGTTTGATGCCCAGGTCAACGAGGTCTCGTGCCTGAGTGTGTCCGACAGAATCGGTTCCACCGGT
CACAGCGGCAATGACAGCAGTCCCTCTGAAGACTCGGTTCTCCTGGAGATGCAGGGTTCCATCCGTTCTGTGGACTATGTTCTGTACCGGTCCATCCAAC
GCAGCAGGGCAGATTACTGTCTCAGTGTGGACTGTGGCCAGTGTGGGCACCATCAGCAGAGCCCGACTCTCAGCCACCAGGGCCCATTTGAGGAGATCCC
ATTGCCTCATGCCAAGGGGGGCAGATCCTTCTCATGCTCCACGCCGAGTGCTGGTGATGATGTTCTGCCGAATGTGGTTGGTGCCGTGGTGACTCGCAGC
TGTAACCGCCTGGAGACCATTGGCCGATCTGTTGGTCCTTGTTTCCCGGAGGTGCGACTGAAAGCAAAGGTTTCTGGGAGGTCTGTACAAAGCAGGGGGT
CCGGTTGCTCCCTCCATCACAGCTCCCCCTGCCAGACACCCGACTGCCCCCACCGCAGGTACAGCGAGAACACAAGGCCGGCTGCCCTTTTCACAACGGT
GCCACCGCGGCTCTTCATCCGCTCCCATAGCGACCCTGGCATAAGCTCCTCCCTCAACACAGATCTCAGTGATGCCGGATACAGCAAAGCGTTAGAGGAC
AATGTGTCGCTGACGTCCATCGATACAGCAATCTCGTCGGAGGCCTATCTGTACCAGCACAGCCCCACTGACCCATTCCCCCTGCTCCGTACAAGCTCAG
CAGGGAAGACCCGGTGCCGCGGGAGCCGGAAACCAGTACAGCGACTCTCCAAAAGTGGCACCTTGTCCCTCGGGGACCTGAAGGGCGGGCGCAGCACCCG
AGTCCTAGTGGCCCGATTCCTGCAGCGATCCAGGAGGAGCCTGAGGGAGGGGTCCGAGAGTCAGAGCCACAAACAGATACCCAGGAGCCCGTTGGCATCA
GAGTGCCCGGAGGGCATATTCCTGCAGAGTTGTGGGGATCTCACGTCTCCCTCTTCGTCCCTTCGCCGTCTCCTTTCCGCCAGTCGGAGGGGCCCCCCGC
GCCCCCACAGCCTGAACACGGAGTATAAGGAGAGCACAGTGTGAGGAGGGGCCCTACCCACGTACCAACCAGGGGGCGGCAAGAACTGTGCCCAACATGA
ACACAATGCACTGTGCCAACCCTGGGGCAGAGCCTGGCACCCTCTGATACAAGGCTGGCAGTGTGGCCAACGCCTGGGCTGTGATGTTGGCAGCCACCAT
ATTCTGATGCCACTTCCCTGGCACACAGTTATACCCAGTCGGTACCCTACATGGAACTGCCCCCCGGGGGGCGTCACAGACAGATGGACTGGGGGAACTC
ATGTGAGGGATCAATGGCTCATGTACCAACGAGTGCAAAGTGCCCAACTTGGCAGCAAACCACCCTGTTTTACCCACAATGCCCCACTCTCCCCAGTTTG
GGCATGTTGGTCAGTTGCAGGGCACTGTTCCTAGGGTGGGCAGAATTAATAATGGCAGTAGTGCCCCCCAGTGTCAGTGCCAGTCCAGGCTGCTGGTTCT
CCCAGAACAGGGTGGCAGTACCAGTATGTGACCCACCTTAGGGTGGCAGTACCAGTATGTGACCCACCGTGGGCACCTTGCCCTCACATTCATACAGGAG
CCTTTGGGAATGCCCCCCTTTACTTTGCATGTTCATTTATTGCTTTGCTGTCAGTCCCGCCTTGTGGGCAGAAAAGGGTTAACCACGGACTGAGAAACTG
CAACATCATGTGGCCCTCGGGGTGCGGCCCCCGGATTGTTAGCTCTTGCGCAATGCTACTGTTAAGTATGTTTATTAATATTTATTGCAGCAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX826029 [+]ctggaaaccagtgatccaacatccttctcttgtgtagggtcctgcccaaccaatgtaaaggctggaagcctctatagactggggcacagttattggtact
ggatttttcttcggaccaggaccttttttccagggctgagttcttggtgccccttccttgggtgcaattccgtccccattttgctgggggagctctaatg
agagaatctagaggctgttggacgcccttgggttcttcagtcggaatctattgacttttctatctttgttccaaacggacctgagtgtttccctcagcag
ttctgccacaggctggggcattatggagtaggatgaaaggttggtctcaccctcctgttccccgtagccaataacgtaggtttggtggccaggacccagg
tctgcagaacttccctgaggaacccttctgctcccagactgggtctccttactgatgagttctgtggttaatctgatgcttctggacccagtgttcttct
ccgtcatgccctctcctacggactccagcttctccctcactggccggacctctcgcagtctgtctcagatccggctccacagaacctggtcccacgtcct
actggccctggggtttgtgcagctcattctgggggtattaatcgtgacgtttagcctggtggctgccactattaccccgtccatgaagatcaagcactcg
tgtccttcctgggccggattctctctggccgtatcaggactgattggctgcatttcctggaggaggcccctgacgctgatgatcacgttattcaccctgc
tgacggcgctgtgcgttatgctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: