XB-CLONE-3646892
Clone Name: IMAGE:7656356
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Gene:
wfs1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154071 [+]
CTGACTCTGCTACACAGGGGCAATCATCTGACGGCCTAGTTATGCCCCTCTGTGCCGGGACTGCTCCCGGGCTCCGCTGCTAGTCTGTGCTGCACAGGCT
GCGACAGGCTTCTTCCTCCCCCCAGGGCTTACAAGAGGGTTCATGGAAAGGACTCAGTGAGATCGTACTGATAAAATGGAAACGGAGCCATCAACACCCC
CTCATCTGCTGGGCAGATCTCAGCTCAATGCCGCAACCTTTGCCGAAGGCCAAGAAGACCCTCACACGTCAGGACCCAGCATGTCTGGTGCAGCATCACC
CCGAGTATTTTCCCCTGTGACAGATGATGTGAATGATGAACTGGACCCTCAAGAAGCTGTATTTCAGGAACTTCTAGAAAGAGCAAAGGCTGGAGATGCA
AAGGCACAAAGTGAGGTTGGAAAGCACTACTTGAAACTTGCTGAAGAGCAAGACGAGGAGGTAAACAGCTGCGCAGCTGTGGACTGGCTTATTGCATCTG
CCAAGCAAGGTCGAAGGGACGCCGTCAAGTTGCTACGTAGATGTTTAGCTGACCGAAGAGGAATTACAACTGAAAATGAAAGCGAGGTGAAGAAACTGTC
ATCTGAGACGGATTTGGAGAGGGCGGTGAGGAAGGCTGCCCTGGTCATGTACTGGAAACTGAATCCAAAAAAGAAGAAACAGGTGTCTGTATCCGAGCTG
CTGGAAAATGTGGGCCAGGTCAACGCTGAAGATGGAAATAATGAGCCAGGACCCATTCCCAAGGCAGCACAGAAGCAGCGGAGGATCCTGGAACGTTTAG
TCAGTAGTGAATCAAAGAGCTTTATTGCTTTGGATGATTTCGTTGAAATTACCAAGAAGTACGCAAAGGGGATAGTCCCAAGCAATTTGATTATACACGA
TGATGAAGATGACGAGTTTGCTGGGAAGGGCCCAGAAGAGCTGCCTTTGCAACAAAAGATCATGAAATACCCCCTCCATGTCATTACGGAGCTGAAGGAG
TACCTGATTGATGTGGCCTCCAAGGCCGGAATGCACTGGCTGTCCACTATCATCCCCACGCATCACATCAATGCCCTGATCTTCTTTTTCATTATCAGCA
ACCTCACCATTGATTTTTTTGCCTTTTTTATCCCTCTGGTGATTTTCTACCTGTCCTTTATCTCCATGGTGATTTGCACACTCAAGGTTTTCCAGGACAG
CAAAGCCTGGGAGAATTTCCGCACCCTGACTGACTTGTTGCTTCGATTCGAGCCCAATCTGGACGTTGAGCAAGCCGAAGTGAACTTTGGTTGGAATCAC
ATGGAGCCGTATTTGTACTTCTTAATATCTGTTTTCTTTGTCATCTTTTCCTTCCCAATCACCGACAGAAACTGGATTCCATGCTCAGAGCTGGCCACCA
TATCCATATTTTTCACCATCACCAGTTACTTGAGTTTGAGCACTAGCGCGGAGCCTTACACCCGGCGCGCCTTGATGACGGAGGTGGCTGCTGGTGCCCT
CTCCCTATTGGATCATTTGCCAATATCGCTGCCCTACCTTAATATCTTCAGTAAAACCTTTTTTACTGTACCTGTTGGTCATTTTATCATATTTCACATA
AGTGTCCCGTGCCTTCTCTTTCTGCACTTGTTCTATCTATTCTTCAGAATGGCTAAACTAAGGAATTTCAAAGGTGTCTATTGTTACCTGGTGCCCTACT
TGGTTTGCTTTATGTGGTGTGAATTATCTGTGGTAATTATACGTGAATCCACTACCATTGGCCTGATCCGGGCGTCTGTCGGCTATTTCCTCTTTCTCTT
TGCTCTGCCGATACTTATTGTGGGCATCGTGGTCATGTGCATTGTCCATTTTATAAAATGGTTCATTTCCCTAGAGCTGACAAAGATTGTGGTGACTTTA
ATTTTATGCACTATTCCTGTACTTCTTCGTTGGTGGTCTAAAGCTAACTATTCCGCCGTAAACATGGTTAAATCATTAACTAAAAGCTCCATTGTGAAGC
TTATTCTGGTTTGGATCACCGCCATAATGATGTTTTGTTGGGTATACGTGTATCGATCTGAGGGCATGAAGGTTTACAACTCTACTTTGACTTGGCACCA
GTATGGCTTCATTTGTGGGCCTAGAGCATGGAAGGAAACCAATATGGCACGGACCCAGATACTTTGCAGTCACTTGGAAGGGCACAGGGTAACTTGGACA
GGCAGGTTCAAGTATGTCCGGCTTACAGAAATTGACAACAGTGCAGAGTCTGCCATCAACATGCTTCCTCTCTTGATAGGAAATTGGATGAGGTGTTTAT
ATGGTGAAGCTTACCCAGCATGTGAGCCTCAGAACGTCACCATGGAAGAAGAGGAGCTTTGTCGCTTGAAGTTCCTCTCTAAGCACTCGTGCCACATGAA
GAGGTTCGACCGGTACAAATTTGAGATAACAGTGGGCATGCCTTTTAACGGGGTAAATGCAAGCAAGCCGATTGAAGAAGATGACATTACGAAAGATATT
GTGCTGAGGGCAAGCAATGAGTTCAAACAAGTGTTACTAAACCTGCGGCAGGGCAGCATCATAGAGTTCAGCACCATCCTAGAGGGGCGCCTCGGCAGCA
AGTGGCCGGTCTTTGAACTAAAAGCCATAAGCTGCTTAAACTGCATGGCCAAACTTACGCCGGCCTCCAGGCATGTGAAGATAGAACAAGACTGGCGGCG
AGATGTCCATACGGCTTTGAAATTTGCTTTTGATTTCTTCTTCTCCCCATTCCTCTCAGCCATTTGAAAATTATTGGACTGATACCCACTGGGGGTCACT
GTGATAAACCAAAGTGTTCTTTCTTTACACTAAGTAGCTTTAACTGTAAGCATACCCTCCGGTTTGCACTTGGTATTGAACTACTGTAGCTTTGGGTATG
TGTTGCTTCAGGAAGGGAGAAGATAGTTACATTAACCTGCACTGTATTTCTCTGCTTCTCACAGCTGCCTTGTTGTGACATGCATGTCTAAGTGGGGTCA
TTCTAAGAAATGGTCCCTTGAGGAGCATTTATCTAACCCTGGGGTGCCCAGTAGGTAGATTGTGATCAGTGTGTGGCCAGTAGATATTGCTCTGGTGGCC
AGTAGATCATCTTTGTCGATAAGAAACTCCGTTAAATCACTGGGTGTTACTCACTATATCTTAGAGTAATACTATTAAACCCTACCGTACATTTGCTCAG
AATGCTCTTCTGAGCACTTTTGCAATTTATAGCTTTGTGCCTAAGTGAGAGCGTCCAGCAGTTACAGCCCATTAGTGGGTCACTGTTGTGATGCCGCATT
CAAAGTATGGGTGCCAGAGGTATTGTACATTGGAACACGTGTTTTGTGCTTTAGCGAGATGCTTCTGCTGGGTGGGTTAGTTGGTTCATATAGGCTTTTG
GCACTTGGCTTCTGGTTCTGCAAATGGCTTTTGTATTTATAACACTTTTTTCCTATGCAAATAATTAACTGTCCCTACACAAAATTCAAATGACCATTGT
ACCTGCTCATTGTTTTGTATACCAAATAGGAACAAATATTGGATGTGGACTTAAATACAATTAAAATGCCTTATCACAAGTCAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
5' EST: CX827515 [+]ctgactctgctacacaggggcaatcatctgacggcctagttatgcccctctgtgccgggactgctcccgggctccgctgctagtctgtgctgcacaggct
gcgacaggcttcttcctccccccagggcttacaagagggttcatggaaaggactcagtgagatcgtactgataaaatggaaacggagccatcaacacccc
ctcatctgctgggcagatctcagctcaatgccgcaacctttgccgaaggccaagaagaccctcacacgtcaggacccagcatgtctggtgcagcatcacc
ccgagtattttcccctgtgacagatgatgtgaatgatgaactggaccctcaagaagctgtatttcaggaacttctagaaagagcaaaggctggagatgca
aaggcacaaagtgaggttggaaagcactacttgaaacttgctgaagagcaagacgaggaggtaaacagctgcgcagctgtggactggcttattgcatctg
ccaagcaaggtcgaagggacgccgtcaagttgctacgtagatgtttagctgaccgaagaggaattacaactgaaaatgaaagcgaggtgaagaaactgtc
atctgagacggatttggagagggcggtgaggaaggctgccctggtcatgtactggaaactgaatccaaaaaagaagaaacaggtgtctgtatccgagctg
ctggaaaatgtgggccaggtcaacgctgaagatggaaataatgagccaggacccattcccaaggcagcacagaagcagcggaggatcctggaaacgttag
tcagtagtgaaatcaagagctttattgctttggatgatttcgttgaaattaccaagaagta
3' EST: CX827514 [+]gacttgtgataaggcattttaattgtatttaagtccacatccaatatttgttcctatttggtatacaaaacaatgagcaggtacaatggtcatttgaatt
ttgtgtagggacagttaattatttgcataggaaaaaagtgttataaatacaaaagccatttgcagaaccagaagccaagtgccaaaagcctatatgaacc
aactaacccacccagcagaagcatctcgctaaagcacaaaacacgtgttccaatgtacaatacctctggcacccatactttgaatgcggcatcacaacag
tgacccactaatgggctgtaactgctggacgctctcacttaggcacaaagctataaattgcaaaagtgctcagaagagcattctgagcaaatgtacggta
gggtttaatagtattactctaagatatagtgagtaacacccagtgatttaacggagtttcttatcgacaaagatgatctactggccaccagagcaatatc
tactggccacacactgatcacaatctacctactgggcaccccagggttagataaatgctcctcaagggaccatttcttagaatgaccccacttagacatg
catgtcacaacaaggcagctgtgagaagcagagaaatacagtgcaggttaatgtaactatcttctcccttcctgaaagcacacatacccaaagctacagt
agttcaataccaagtgcaaaccggagggtatgcttacagttaaagctac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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