XB-CLONE-3644425
Clone Name: IMAGE:7657978
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Gene:
entpd4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136125
[+]
CTGAAATCCCTCCAGAAGCTTTTCTAGCAACCAACCCTACAGGGAGACTTTGGTACTGAGAACTGCATTCCCCAAGCAAGTTACTGTGCAGCGTGGCTTC
TCCTGCCTCACAAACCTGCAGTCTGCCTGGTGAGAGATCGAACAGAGGTAGCCCGGAGTGATTGGCATCTCCTGACCTGTAGTGACGTGTCCGGTGTCGG
CGTGTGCGGAGCGCGGGAGTAATAACAACAAAATACCAGTAACAACAACATGGAGCAGAAATTCTGCAAATGACATAGGAAACACCACCCACTATGGGTT
GAATTTCTTCTACCTGTGCTGCTAACGTGCTGGGCTTCCTCATAATGGGAAGGTTAAGTATTTCTTGCCTGGTTCCAGCTTCATGGCACTTCAGCCTCTC
TGCAACTGGGTTCCCTCGCCTCCTAAACCCCACTTTCCGTCAGCTGATGGTGATTGGCGCAGCAGCGGTGATCCTCCTTTTACTCATATACTCTCTGCTC
ATGGCCAGGAGCAAACATGGCGGTTCTTACCTAGACATCAAATATCAGAGGTACCTGGCTCGTGTTACAGACATGGATGCCACAGACACAAGCAATCCCA
ACATTAATTATGGCATAGTTGTAGACTGTGGAAGCAGTGGCTCACGGATATTTGTGTATTGCTGGCCACGTCACAATGGGAATCCACATGAACTTCTAGA
TATCAAGCAGATGAGAGATCAGAACAGGAAGACTGTCGTCATGAAAATCAAACCAGGTATTTCAGAACTTTCTAGCACCCCAGAGAAAGCCAGTGACTAT
ATCTCCCCCTTGCTTGACTTTGCTGCCCGCCACATCCCCAAGAACAAGCACAAAGAGACCCCTCTGTATATCTTGTGCACTGCAGGGCTGCGAGTCTTAA
CTGAAAGCCAACAGGAGGCCATACTGGAAGATCTGAGGACGGATATCCCGGTGCGATATGACTTTCTTTTCTCTGACTCACACACTGAGGTCATATCAGG
GAAACAAGAAGGAGTTTATGCCTGGATCGGCATTAATTTTGTCCTGGGGCGCTTTGATCATATAGAAGAAGAAGATGACGCTGTAGTGGAAGTGAACGTC
CCTGGGGCTGATAATAAGGAAGCCATCCTGCGCAAGCGCACAGCAGGCATCCTAGACATGGGTGGCGTCTCCACCCAGATCGCATACGAAGTCCCTAAAA
GTGAAGAAGTGGCTAAGAACCTGCTGGCGGAGTTCAATCTTGGCTGTGATGCGCACGAGACGCAACACGTTTACCGTGTGTATGTTGCCACCTTCCTCGG
TTTTGGAGGTAACGCCGCTCGCCAGAGGTATGAAGATCACATCTTCTCCAGTAACATGGAAAAGAACAGGTTACTGGGGAAGCAAATTGGCATGTCTGGG
GATGCTCCATACTTGGACCCCTGTCTTCCTCTGGATATCTCTGATCAAATCCAACAACGAGGGCAAACTATGTACCTGCGTGGCACAGGAGACTTCCAGC
TTTGTCGCCAGATCATTCAGCCCCTTATGAACCTATCTAATGAGACACAGACCACCTTGAATGGCGTTTACCAGCCACCAGTTCCCTACCACAACAGCGA
GTTCTACGGTTTCTCAGAGTTCTATTATTGCACTGAGGATGTTCTGCGTATGGGTGGGAGCTACAACTCTACTCGCTTTCTGCTGGCAGCACAAGATTAC
TGCGCCACCAAGTGGTCTACACTGAGGGAGAGATTTGACCGTGGCCTTTACGCATCACATGCAGACCTGCACCGTCTCAAGTACCAGTGCTTTAAGTCTG
CCTGGATGTATGAAGTTTTCCACACAGGATTTTCCTTCCCTAGTAACTATGGCAACCTCAAGACTGTTCTGCAGGTGTATGATAAAGAAGTGCAGTGGAC
CCTGGGAGCCATATTGTATCGGACCAGATTCTTGCCATTGAGAGATATTCAGCAAGAAAATTTCCGAGGCAACCATCCCCACTGGCGAGGGGCGTCCTTT
GTGTACAATCACTACCTGTTTCTGGTGTGCTTTCTGATTGTCCTGCTGTCCATTGTGCTTTACCTGTTGAGACTTCGACGCATTCACCGCCGTATGCAGC
GCAACACCTCCTCTTCTGCTCTGTGGGTAGAGCAAGGACTTCAGTCCCCCGGGCTATCAAATGTTCTATGAGCCACCGCAATACCCGCACACTTCTCATT
GGATCGGCAAGAAGATTGTATGGACTGTGTAGCAGCTCCCTCTTATCTATAAAATATCTGGATCTTATGATCCAGTGTGGCTGCCATAGCCCTGGCATGT
ACAGATGCATTTAACAAAGCCATGTTTTTAGACTCAGGGTTACCTTTACCCAACAGTAATAACTGAGCGTGATGGAACCCTCACTACAGTCGATCAGGGC
ATATCTTATATGAGGATGGAAGAACACTTGTCACCATCTACGTGGTCACTACATCCTCACACGCAAAAAACTTGACATGAAATTGTCATTCAGGTTGTTT
CAGAACTCAGAGGCCCATGGGACCTTGCTTAAATATCATGGTCATGGTCATAATCTAAATGGGAGATTCTATTCTAAAGAGTTACTTCACCCTTTGGAAT
ATATTTCTGAGAAGAATGGAGCTTGAGCGGCTAAGGAAGCCACTTTCTCTCTAGTGTCTGCAGTGTTCACTTTGTTCTAAAATGGGTCAGTGTTGATGAT
GAATAGGGTAGTGTGACAGACATATGCGATTTCTTTTCCTTGGCCATAGCGCAGTGAGATATTGTTTAAGGGATCTGTACCTATGTGTTAGTGCCCTCTC
CTAATTCTGCCTTATGAGGAAATTGTTAAACGGTAGGTGACTGTTGCATTGCTCGTCCCTGTATATTTGCTGATAGAATTTTCTATTTGGTTCCTTCATA
TGAAGTCCAACCTTCCGTCCATAAAAGGCCTTGTACGATATAGTATTTTTACTATATAAAAAGGGATGGGGTGTGTGATTACTGTATGATCAAGCAGCTG
GCAAATGAAGTCCGCATTGCTGTGCGCCTAAACGATAACGGACGAATACCTGCTGCTGTGCGTTTGTTAGGGGCACCATCTTTGTCTCTGTTCACATAGG
GTATTTGTTTTGTTTCCTTTCATAATTTGCATTCACATTAGATATAAGGCAGCTGTAGTTTCCAGGATACCTTGGGTGGGGTGTGTGCAACTGTGTATTG
TGATGGTGAGTAACTGCACACATATAAATCATGCCACTCCGCCTCATTGTCAGCTGCACTTTAAACTGTAGTTAAATAAGGATTTACTGTCTAAACTGCC
TTTTTGTACATTCGGAATTCTTAAGTACAGCCTTCTGCTTCTGGCCTTTACCTGTAATTACGTGGTTTACAGGATCTGTCTCATTAAAGGAAAACGTGCA
CCTAAGACTAAAGTAATATCCACAAGGCCTCTATAAATTATATATATATGTGTGTGTGTGTGTATATATATATATATATATATATACACACACACAGTAC
GGTCTGGTTATTGTAATTAAATACATGCAAGAGAGCACCTTTTTTTAAAAAAAATGTAATGCATCTGTTTCATTATTATATAGCGAATTCCACTGTAACA
ACTGCATAAAACAACTGCATCCAATCCACTGCTCAGGTGGATAAATCTGCCATTTATTTTACACAGCAATAGTGTTTTGTTTTTTTTTACATTTTTTAAG
AAGTAAAATGAAAGAATCTTTGTGTGTAATGTGCCAAGTGTACTATCCTGACCTGGCACATGTGGGGGTGCAATCCTTACAGAAGCACAGAGGTTCACGT
AATATATTGAAGGATTTGTTTATTGGGATAATTCAGGAAATGATGAGGGACGTTTGTTTCATGATTTTTTTTTTCTGTGTGTTTTCCACATTTTTATTTA
TTAAATAATTTATTTGCCATAAAAAAAAAAAAAAAAA
5' EST: CX830286
[+]ctgaaatccctccagaagcttttctagcaaccaaccctacagggagactttggtactgagaactgcattccccaagcaagttactgtgcagcgtggcttc
tcctgcctcacaaacctgcagtctgcctggtgagagatcgaacagaggtagcccggagtgattggcatctcctgacctgtagtgacgtgtccggtgtcgg
cgtgtgcggagcgcgggagtaataacaacaaaataccagtaacaacaacatggagcagaaattctgcaaatgacataggaaacaccacccactatgggtt
gaatttcttctacctgtgctgctaacgtgctgggcttcctcataatgggaaggttaagtatttcttgcctggttccagcttcatggcacttcagcctctc
tgcaactgggttccctcgcctcctaaaccccactttccgtcagctgatggtgattggcgcagcagcggtgatcctccttttactcatatactctctgctc
atggccaggagcaaacatggcggttcttacctagacatcaaatatcagaggtacctggctcgtgttacagacatggatgccacagacacaagcaatccca
acattaattatggcatagttgtagactgtggaagcagtggctcacggatatttgtgtattgctggccacgtcacaatgggaatccacatgaacttctaga
tatcaagcagatgagagatcagaacaggaagactgtcgtcatgaaaat
3' EST: CX830285
[+]ggcaaataaattatttaataaataaaaatgtggaaaacacacagaaaaaaaaaatcatgaaacaaacgtccctcatcatttcctgaattatcccaataaa
caaatccttcaatatattacgtgaacctctgtgcttctgtaaggattgcacccccacatgtgccaggtcaggatagtacacttggcacattacacacaaa
gattctttcattttacttcttaaaaaatgtaaaaaaaaacaaaacactattgctgtgtaaaataaatggcagatttatccacctgagcagtggattggat
gcagttgttttatgcagttgttacagtggaattcgctatataataatgaaacagatgcattacattttttttaaaaaaaggtgctctcttgcatgtattt
aattacaataaccagaccgtactgtgtgtgtgtatatatatatatatatatatatacacacacacacacatatatatataatttatagaggccttgtgga
tattactttagtcttaggtgcacgttttcctttaatgagacagatcctgtaaaccacgtaattacaggtaaaggccagaagcagaaggctgtacttaaga
attccgaatgtacaaaaaggcagtttagacagtaaatccttatttaactacagtttaaagtgcagctgacaatgaggcggagtggcatgatttatatgtg
tgcagttactcaccatcacaatacacagttgcacacaccccacccaaggtatcctggaaactacagctgccttatatctaatgtgaatgcaaattat
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: