XB-CLONE-3642421
Clone Name: IMAGE:7658854
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Gene:
cdc14b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136223
[+]
AATTAATACAAATTATTTCCTTTTATGAAGAAAGGCATACTATGAATTGTTCCCAAAGTTAAAATCTTTCAATGTTTTCTGAGCTGTTGTAAGATAATGT
TGTTTTCCATTTCTCTGCCTTTCAGATCGCCTTTATTTTGCCATCCTATGCAGTAGACCAAAGACCACCTCTGGCACACATTATTTCTCCATAGATGATG
AGCTTGTATATGAAAACTTCTATGCAGATTTTGGACCGCTTAACCTGGCCCTGCTATACAGATACTGCATGAAGTTAAACAAAAAAATCAAGTCCTTTTC
ATTGACCAAGAAGAAGATTGTTCATTACACCTGCGGTGATGATAAGAAACAAGCCAATGCTGCATTTTTAGTAGGAAGCTTTGCTATAATCTATCTAAAC
AAGCAGCCTTTAGAGCTGTATCGGCTTTTGCAAACAGCGAAAACAAACTACCTTCCTTTTAGGGATGCTTCATTTGGTACCTGCACTTTTCACTTAACCC
TTCTTGACTGCTTTTTTGCTGTGCACAAGGCTTTACAGTATAACTTCTTTGATTTCAAAACTTTTGATGTGGAGGAGTACCAACATTATGAGAGAGCTGA
AAATGGTGACTTCAACTGGATCATCCCAGAGAAATTCTTAGCTTTCAGTGGGCCTCATCAAAAGAGTAAAATAGAAAGTGGTTACCCACACCATGCTCCA
GAAGCCTATTTCCCTTACTTCAGGAAACACAATATTACTACTATTATTCGCCTTAACAAGAAAATGTATGAAGCAAAAAGGTTTACTGATGCCAATTTTG
AACACTACGATCTCTTCTTTGTTGATGGAAGTACTCCAAGTGACGCAATTGTCAGGAAATTCCTCAACATATGTGAGAATGCCGAAGGAGCAATAGCGGT
ACACTGCAAAGCTGGTCTGGGTCGAACTGGCACGTTGATAGGGTCCTACATGATGAAGCATTACAGGATGACTGCTGCAGAAACCATTGCATGGATCCGC
ATATGCAGGCCTGGCTCTGTCATTGGTCCTCAGCAACAGTTTATGGTTGAGAAGCAGCGCAGCCTATGGAATGAAGGGGATATTTACCGGAAAAAGTTAC
AAGAGCAGGAGAATGGAAACAGATGCGCAGTCACGTTTATTCTTTCTGGTGTGGATGACATTTCTATCAGTGATGATAAGGGCAAGGCTTGTGGGAAAGA
AGATATTGGACTGTACAGTGAGGATGATGATGATGAATCCATCCATGTGACTCAAGGGGACAAGCTTCGTGCCCTAAAGTGTAAACGTCAAACAAGAGTA
TCAGGGGCAGCACCTTTATCTCATTCCATTCCAAAGACTAGAACCGTGCTGCGATAAGAACAAGCCAAGGGCTGCCTTTGCCGAAGTGAACACAACAGAC
TTATAAACCAAACTTTATGTCCTTGGACTTACAGGATTTGCAGCCTATAGTCTTTAATCCCCCAAACTGTCTCGCTCCACCTCACTCGCCAGCAGCATGA
AGGTTACTTGTATTATCCAATGTAGGAATGGCCAGTAAACTACAACTTCCTATGGCTGTCAGGAGGAACTGGAAGACATTGCCCCCCACCCTCCTTTATC
AGAACTCTGCTACTAACACAGCTGTACTGTCTCGGGCACAAGGTATACAAACTGCAGCAAAGGAATCCAGGGGGGCACTCTGCATGGTTTATAAATTGTG
ATGGCAATCATTATAGTGCTAGGATGACACTGAGCTCTAGATTTCTACTGTCTTGGCCGAAAGCATACACAGGTAACCAGGACTGCTTCATAGTGTCTGA
CACATTTTGTGCCGACTTGTGTTCATTTCTCTTCTCATTTCTCTCTTCTCAGTATTCCTTGTTTAAAAGGAAGCAATGTATATAATGTGTAAATATCTAA
TCATTTTAATGTTCACTAGACCAAAGTGTTCTTCCTAACGTGCATTTATATTATGTTGCAAGTTTTTTTTTTTGTTTTTTTTTTTTTTTAATAATATAAC
CTCAGGCGCATGGGCATGTTAATTTGTAGAGAAACTGAAAAAAAAGATTTGCACATCTAAAAAAAAAATTAATGAATTGGGAAAAAAAAGTGTATATTTT
GTGCAAGCCCCTGTGCAAATATCAGGAGCCCCACTTGGGGACTTATGGTAAAGTGGAGGCCATCTAGATGACGAGCTACAATTTCCAGCACCTACTGTTG
GGAGTTGCTGTTCAGGCACATCCAGAGGGCCCCTACTTTCCCATGACTGAATTAAAGCATATTAAGCCCATGTAATGTCCTTTTGGAGGGCACCACAATA
AGGGTTGGGTAATGAACTGATGAAAGATGCTGCTATTGCCAGGTGCAGAGTTGGTCTGCCAACAGTAATATGGTCACTTACAGGTTTTCTGTCTTCTCAA
CCTGTCCCTCCAAACTGTACTTGCTTCTGCAATGCATTGTGATGCCAACTGAAAGGAAGTAGAGGTCATGTGGGCTCATGTGCTGCCACATAAAATGCTC
CTAACGACAAAAGGAGTAGTAAGACTTGCAGACAAGACATTCGGAAGATCAGCCTATCCCTGCTACCCACTTGTATTTGTGATCATGTTAACTTGCTGTG
CTTCTGAGTTGGCATGTTAGCCCCAGACTGTCCAGGCACAGGTTATGGAAGTTTTTTTTTTTTCTACAAAAGAGTCTAGATAGTAGCACTGCTTGCTCCT
CTGGGTTTGGATGCTCATTGTATTTCTGTACATAAATTATATATATATATGCCTTCACCAATATTGCCCTGCAATGTGACATTGCTGGCAATTGCAAAAT
GAATTTATATGGCTCCCCATACTGAACACATTGATTAATGGTAATCCATAATTATTGTGGCAAGTCGAAGTGCTTATTTCTTTCTAAAGCTACACTATAT
GTTCAGAGCACACGAGTTTATAAGTGCAAGTTAGAGAATAACAGTAGGGATTAAAAGTGAACTACCCCCACGGGCACATACCCCATAGGGCATATTGTTG
CACTGCATGTAGTCATTAGTCCTGTTCAGCCATAGGTAGATACGAGAGTCTCTCTCTGAATTTAGAAAAATTGGCTAAATATATCTCATTTGTCTAACAG
TCAGAGAAATTATAGATCTTTTTAGAGTTACTTTAATCTCCTCCATGGGTCCAAGAAATTCTTTCAGTGCTGCGAGGATGATGGTAACAGAACATACTGT
GGCATTGGTCTTGCATTTAATGACTCGTTATGAGGAGTTTAATGCAATGAATTTGCAGCAATTAGATTATTCAGCCATTTTCAGACTTTAAAATGCTGAT
GATGAAACAGATTCTGATTTTGGGTGATGTGCCCTCTGCCTTGCACTAGAAGCCAAGCATAAATACCAGGGCATGGAGGGCAACCTCTAGCCCTCTGGAA
ACTAGAGCCAAAAGAGTCTAGGATTGATTATGATGATGAATTACCAGCCCCATCACTGTTATTCAGGAATCCAGTCCCTGTTTGCCTTTACATGGCGCTA
CCTTGGGGGTCTTCTACTTTATATTGAATTGACTTGAAGTATTTATGCATATATGCTATTCTCTAGTTCAGCAAGATTTAGTTTCTTTATTTCCCATTTC
AGTTGCAAAGGTTTTTTTTTATCTCTACTCTCCAAAACAGTGGTGATTAGGATGTAATGGGCCAAGCTTTGTAGCGCTCCATTTTCTTTGTATCCAGTTT
GTTGATAATAATGGAATAATAGGAGGAAAGGTATCCTTAAAGACAGCCAGTAGATTTTTTTTTTTTTTTTTTATTATCCATTTTTAATTGAGAGACTTCT
CTTGCTGCACTTGTGTACTCTCCCAGAATCCCTTGCTTCCATGTTCTAGATTTGCACACATATAGTAAATAGTTGGAGCACTACAGAGGAGGGAAAACTC
AGGGATAAAGGGCAGTGAAGAACTGTTGTACCTGCTATAAAGGCCTGTATTACTGGGTAATTCTGAATGTTGAAGTGTAGAGCAGTGAGGCCTTTACAGC
AAATGGTTTTTGGTGTGAAGATTATGGAAGCCTTTCCGCTCACGCACACCCATGTTTAGCATCACTGTGCTCATGTTACGGTTCTCGTGCCTCTTTCATG
TACAGAATATGTTTTAAGAGCTTATTTAACCCATATCCCCATTTCTTAATTTTTAGTATATTGTATATTTGAAATCCTATTTTTTAAACTGGAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX838564
[+]aattaatacaaattatttccttttatgaagaaaggcatactatgaattgttcccaaagttaaaatctttcaatgttttctgagctgttgtaagataatgt
tgttttccatttctctgcctttcagatcgcctttattttgccatcctatgcagtagaccaaagaccacctctggcacacattatttctccatagatgatg
agcttgtatatgaaaacttctatgcagattttggaccgcttaacctggccctgctatacagatactgcatgaagttaaacaaaaaaatcaagtccttttc
attgaccaagaagaagattgttcattacacctgcggtgatgataagaaacaagccaatgctgcatttttagtaggaagctttgctataatctatctaaac
aagcagcctttagagctgtatcggcttttgcaaacagcgaaaacaaactaccttccttttagggatgcttcatttggtacctgcacttttcacttaaccc
ttcttgactgcttttttgctgtgcacaaggctttacagtataacttctttgatttcaaaacttttgatgtggaggagtaccaacattatgagagagctga
aaatggtgacttcaactggatcatcccagagaaattcttagctttcagtgggcctcatcaaaagagtaaaatagaaagtggttacccacaccatgctcca
gaagcctatttcccttacttcaggaaacacaatattactactattattcgccttaacaagaaaatgtatgaagcaaaaaggtttactgatgccaattttg
aacactacgatctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41414.jpg) |
Usage:
Suppliers: