XB-CLONE-3640756
Clone Name: IMAGE:7660149
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Gene:
spred1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX840331 [+]
ctcgcacggatgcttggcgcaggattggcctgcctttaaatcgcttctccgcagtttatcgctgtttcgaatgctccggcggggcgcacttcccatataa
ccttccgattgcctcccggatccctgtgccctcaccatgcgtctccttcgggagcctccctgcgccctggccgacggcagccagccccgaatcggagccc
ggtgaaaggggacaatgagcggcgaacaggagccagatgacagttatgcacgagtgcgagctgtggtgatgacaagggatgactcgagtggtggttggct
acaacttggaggtggagggctcagctctgttactgtgtctaagactcttcaaccaggagacagtggtggcacagaattcctagttcatggcgagagactc
agagataagacggtggtcctggaatgtgtgctcaggagggatcttgtctacaataaagttacccctacattccatcactggaggatcggagacaagaagt
tcggccttacgttccaaagtcccgccgatgcccgagcttttgatagaggaatacgtcgtgccattgaagacctgtctcaaggcttaccagcatcttgcca
tggagagtcagaaacttctgaggatggaccacaggttcctctcaagccaattcgacatgtcagcttccaggatgaagatgagatagttcgcattaacccc
cgtgacatgattattcgccgctatgcagattaccggcacccagacatctttcggaatgacgttgatcgggaggaaccagaagatgttacattctttacca
gacggacagtanaaagcccagctatctctactcccca
3' EST: CX840330 [+]atctgacagttgtgttatattctatgtaccagagtgtataaataatgtgacataacacaggtagatccgagagacagaaaatccggattaacagtaaatc
ttctgaaaaacaatacactggaaaaaaaacagctttttaaccattctgctgccagaaagattgcccctctatagcagcaggactcagccctataatggaa
tgtgccaattttcattaggaaaacagtggtggaagctgaataaggagcataagaaacaccctcccaaaagtcataaaattctgcgtaccgtatatttagt
taaatatagacatttgttatttatacataaaatcacttgaataaaactagaaatgatttagttccactaaaaactcattttaggtaaccgcgtgtctgtt
gcttgtccgtttcctccacgtcacatttcttttgtatacaatgttagtcatccccccagaatcaacagatttctcatattaaactgctttctggagagga
gatgtaatacggaaaatatatatatatatattgttctataatcattttctcacttccttaagcctacagcctaatggatagagatccattcattgtatca
tcctgccttgtggaattcctcacagttcattatcccgctacaaggcggccaatctgaaaaaaacaaattctctgcttttgaacaggaatggcaaataaaa
aataaaataatattaattacaaaaagaaaaaaaataatatatatatatatatatatctatcgcaagactggcaccgntacatattcttgctggtcctatc
tgaacctagacagcaccatttagtcctc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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