XB-CLONE-3639522
Clone Name: IMAGE:7660863
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Gene:
atp6v0d1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC125665 [+]
AACGCTACCATCATGACATCCTTTTCAGAGCTGTACTTCAACGTAGACAGCGGTTATCTGGAGGGACTTGTCCGGGGATTCAAGGCCGGCATATTATCTC
AGGGAGATTACCTTAACCTGGTGCAGTGCGAAACCCTTGAGGATTTAAAGTTGCATTTGCAGAGCACAGATTATGGTACCTTCCTGGCCAATGAAGCGTC
ACCCCTTGCTGTCTCTGTTATTGATGATAAGCTAAAGGAGAAGATGGTGGTAGAATTCAGGCATATGAGAAACCAGTCATACGAACCACTGGCAAGTTTC
ATGGACTTTATCACGTACAGTTATATGATTGACAATGTGATCTTACTTATAACTGGAACACTGCACCAGCGATCCATCTCTGAATTAGTGCCCAAATGCC
ATCCTTTGGGGAGTTTTGAACAAATGGAAGCAGTCAACATCGCTCAGACACCAGCAGAACTCTACAATGCTATCCTCGTCGATACACCACTAGCTGCATT
TTTCCAGGACTGTATTTCTGAACAGGATCTTGATGAAATGAATATTGAAATCATAAGAAATACTCTTTACAAGGCCTACCTGGAATCCTTTTACAAGTTT
TGTGATTCACTAGGTGGCACAACTGCAGATGCAATGTGTCCAATTTTAGAGTTTGAAGCTGACAGAAGAGCATTTATAATCACAATAAATTCATTTGGCA
CTGAGCTTTCAAAAGAAGACCGCGCCAAATTGTTTCCGCACTGTGGAAAACTTTATCCAGAGGGATTGGCCCAGCTAGCACGTGCTGATGACTATGAACA
GGTCAAAACAGTAGCTGACTATTATCCAGAATACAAACTACTGTTTGAAGGTGCAGGTAATAACCCTGGTGATAAAACCCTAGAAGACAGGTTCTTTGAA
CATGAGGTAAAGTTAAATAAATTAGCTTTCCTAAACCAGTTCCACTTTGGAGTTTTCTATGCATTTGTAAAGCTGAAGGAACAAGAATGCAGGAACATTG
TGTGGATTGCAGAATGCATCGCTCAGAGACATCGGGCCAAGATTGACAACTACATCCCTATCTTCTAAAGTCACATTCCAACCTACTTTCCTGTATATTT
TCCACAGGCACTGCAATACCTCCTTCACTTTACTTTCCATATCATAGGCAGGGGAGACAAACCATTTTAAATGTGCAGTGAAGTTTAATTACCCTCTGCT
TTTTTTTGTAGTTACCTCAAAGAACCACAGGAGATTTTATTTAATATGTGCAATGCCAGTGGCCCTTTTTCCTTCTTCACATGAGGTGTGATGCATGTTA
TGCATATATGACAAATATACTATGGTTAGATTCAAATTCTTTAATATAAGGGGTATACGTAAATTGAGACTTATCTGTTAAGGCTGATGCACACAGACAA
TTTTCTCTTTACCCTCTGTTTTTAGATTAGGGTAGAGGCAGTAAGTACAAGCAGACTGCTAGTGCAAGCACATACAGGTCAACAGACATTGTCATGTGTT
TTTGCCATGCCAGTGCTTTTGTCACAGGTCACAGGTCTTGCATCTTGGCAGGAGATAATGTGCAAGCTGGCACTGAGAAAGCTTCTATTTACTCAAGGTC
TTACAAGGAAATTAATTTGTGGCCAACCCATTAACTGAATTTTACAACAGAATGTATAATGATTTTTAACTTAAAGGAACAGTAAGGGTGAAGACACATG
GAGCTACTTAGTAGCAGCTAACACCAGGAAATAAAAATGTTTTAAAGTAAAGTGCGCTGGTAAAAGTTGGGTGTTTTCTTCGGAGGCGGTACTGTAGTTT
GCGTAAACAAGGTTTCTGGGTAGCCATGGAGGCAGCCATTCAAGCTTGAAAAAAAGGAGAAAAGGCACAGGCCACATACAGATAAGTTCTATATAACATA
ATTGGAGTCTACACATAACATAGTGTAACATAGTTATATATACATCTGATGTATAACTTCAGCCTTTTTTCAAATTGAATGGCTGCCCCCAGGCTGCACG
GCAGCTTTGTTTGTGTGGGCTATAGTATTGTTTCTGAAGCAGACACACAACTTTTACCAGTGCAGGGCAATAGTGCATAATATAGTAATTATTTTGATAA
ACTTAAATTTTTTGGTTTTACTGTTCCTTTAAGCTTGTGACCTATGCCGTAAATATATTATTCCCATGAAAGGTATAGATTGCCACCAGTATGATAGACT
TTCTAAGGCATCTTTGTGAACCGCCTAGAAATAGGTGTAATGTATTTGTTTTATGTGGTAACTTTGTCACTGTATGAATTGAATTGTTTACATGAATAAC
TCCCCCCCCCCCCCCCAATATCTTCAGATGGCTAGCTATTCAGTAGTTGGAGGAAGGTTCAACCTTATCCAGTTGGAGGGTGAAATGGTCATTTCCAAGC
ATCAGAAACTTCACAAACTTCATGATCTGATAATCCAGACAAGTAGCCAAGCTTTCACCTTCATGTCTGCCCTGTAATTCTGATGTGATGAAACATAATA
TATTATTTTATTCAGGTGAACTCATGACTTTATGTGATTTCGCCTCAAATTTAAATATCCAAATTTGCTCTCAGGTTCAGTATTTAGGCTGATGGCGGAC
GAGGAGTAGGGCGGATGTTTTTGGCAAGCGGAAAAGCGCTTGCGGTTTTATGCGTATTTTGGCTACATGTGCCTGAACCCGAGCGCATTCCATTCATTTG
GGTGCAGGCACATGTAGGAGGTGTAGGGCGGTATTTTCAGCAAGCGCTTTTCCGCTTGCCGAAGGTGTCCGCCCTGTGCCTCGTCTGCCATCAGCCTTAG
ACAAATCCATAGATTGTGAATACAGGGCATTCCCTAGTTACTAATACTTTCCTAACTGTCATGAATATGTATCACACAGTGAAGACAAATCTGAACAGTT
TGCCACTTACTGGAGTTTTTGTCTGGGTGTAGGAATTATTGTTCTTACAAGGGCTACTTTAGTTATCCTGTCCATCAGTGCTCCTTAGTACTACTATGTA
TATATATATATATATATATATATATATATATATTATTGAGCTAGCCTTTAGGCGGAGCACATGGCTTTCCAGACACACAGGCTCCCTTCCAGTTCTCTGG
CAATTTCATGAAATGCACCATAAAAACCTACTGTTTTTTTTTTTATTTATGGTGCAACAGCTCACTTAGAGATGTTACATCGTAGAAGTTGCATCATTAA
TCTTTACCATATGACAGTATGGTGTATAAACCTTTAATCACTATGCCTAAATGTAACATCTATGCGATCTGATCACAAACTTTATCTCTGCGCTGGTGCT
CCTGTTAGGGAAAAAACATGCAAAACCAGGGAAAGATGTAATACAGCTTGTGCCCCATCTTGCTTTTCAGACATGAGCGTTTGTGCAGTAGAGTAAAAAA
CTGCATTTTACTGCACATATCTGAATTTGAGTCTGCCAGCGAATGACTGGGACAGGTAAAATGGCACTACAGCACTGCTTTACTTTTTTGGTACCCTTTT
GGGTCATTATAGGGACAGCAGGTTTTACATAATTCTAAGGTTTAAGAAAAAAAAAGCGCATTTTAAGGGAATGATTCGCTTATCAGCACCCATTAAAAGA
AACCTAAATCGTATGTTTTAATTTTATTTTGCGCATTATAACTGTCATTAAGCAATGATGTAATGCTTCATGCATACAGTATACAGTAGATTCCAGCATA
GTCCCAATTAATCACATGAGAGTGTTTCCCCTGTATGATGTGAAGACAAGAACATTTCGTTTGATGTGGAAATCCATTGCCCTTCTTGTCATTTTGGTTA
ATTTTCAGAATTGTGGCATTGAGTGGTATTTTTATATTTTCTGATTTTCAGTGTTAAAGAATATAACTGTTATTCTGGAAAAGAAGATCACCATATTATC
CTTATATGTGCAATGTTAAAAACGATATTTAAAGATAAATCCTATTTCACTGAATGCATTCTCTTAAAATCTTATATATGTAAAAATGTGGTAATATAAT
AAAATGTTTTACAGCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CX841709 [+]aacgctaccatcatgacatccttttcagagctgtacttcaacgtagacagcggttatctggagggacttgtccggggattcaaggccggcatattatctc
agggagattaccttaacctggtgcagtgcgaaacccttgaggatttaaagttgcatttgcagagcacagattatggtaccttcctggccaatgaagcgtc
accccttgctgtctctgttattgatgataagctaaaggagaagatggtggtagaattcaggcatatgagaaaccagtcatacgaaccactggcaagtttc
atggactttatcacgtacagttatatgattgacaatgtgatcttacttataactggaacactgcaccagcgatccatctctgaattagtgcccaaatgcc
atcctttggggagttttgaacaaatggaagcagtcaacatcgctcagacaccagcagaactctacaatgctatcctcgtcgatacaccactagctgcatt
tttccaggactgtatttctgaacaggatcttgatgaaatgaatattgaaatcataagaaatactctttacaaggcctacctggaatccttttacaagttt
tgtgattcactaggtggcacaactgcagatgcaatgtgtccaattttagagtttgaagctgacagaagagcatttataatcacaataaattcatttggca
ctgagctttcaaaagaagaccgcgccaaattgtttccgcactgtggaaaactttatccagagggattggcccagctagcacgtgctgatgactatgaaca
ggtcanaacagtagctgactattatccagaaatacaactactgtttgaaggtgcaggtaataaccctg
3' EST: CX841708 [+]aaacgcgggaaaacattttattatattaccccatttttacatatataagattttaagagaatgctttcagggaaaaaggatttatctttaaatatcgttt
ttaacattgcccatataaggataatatggggatcttcttttccaaaaaaacagttatattctttaaccctgaaaatcaaaaaatataaaaatacccctca
aggccccaattctgaaaattaaccaaaatgacaagaagggcaatggatttccccatcaaacgaaatgttcttgtcttcccatcaaacaggggaaacactc
ccaggggattaattgggactatgcgggaatctactgtatacgggatgcatgaagcattacatcattgcttaaggacagttataaggcgcaaaataaaatt
aaaacatacgatttaggtttcttttaaggggggctgaaaagcgaatcattcccttaaaaggcgctttttttttcttaaaccttaaaattatgtaaaacct
gctgtccctataatgacccaaaagggtaccaaaaaagtaaagcagggctgtagggccattttacctgtcccagtcattcgctggcagactcaaattcaga
tatgtgcagaaaaatgcagttttttactctactgcacaaacgctcatgtctgaaaagcaagatggggcacaagctgtattacatctttcc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: