XB-CLONE-3639454
Clone Name: IMAGE:7661065
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Gene:
txndc16
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC121862 [+]
GGAATAGGATGCTTTCAGAGGAAGACTGGCCGCAGAGTATGGACCTTAACCTGGGATGGCTGTAAATCTGTGCAGCTATGCCATGTCTTCAAGAACCTGC
TAATTAGAATCCTGAGCAGACACTGAACAGGCTCATACCTCTTTCATGCTTTCAGATGAACAATCTTTTTTAGGGCATGAGCAAATTGCTGGTCCAGATA
AGTAGTCTCAGTCACCTTGGTGAAATCACTTGAAGATATGGTGATTCCAGCTGATCCAAATTAAAATACCTTTTAAATTAAAATGGCTGGCCTGATCCGT
TTAGCTACTTTTTTGTGTGTAAATAGTATTTTTATGACAAAGGCAATAAATACTTTGGATGAACTAAGATCAGATACCTATTATAGTGCCCTTATGCCTG
GGAAGACATCACTACTTTATTTCACTAATCCAGCTTTTCCTAGTAATATTATTTTCCTTGATGAACTCCAAAAGTCTGCTGGGGCTCTTCAAGACTATGG
GATTTCCGTCGCTAAGGTTAATTGTATGAAAGAAGACCTACTAAAGTACTGTGGGGAGGAGAATGCATATTTGTTTAGGGGTGCTAAGTTAGTAAGGGAA
TTTCCAACAGATGCACTTTTTGATGTTGATTCTATCGTGGCCAATGTCTTATTTGTTCTCCTTTATAATGAAGTTAAATACATAACCACCTCTATGGAGC
ACCAAAATATTGAAAGTGCTATGAAAGGGAAAAAAGACTTAATTTTTGCCTACGTTCAAGCAATAGGAATACCAGAACATAGATCGGTTATGGAGACTGC
GTTTGTTTATGGATCCAAATACCAATTTATTTTAACCACAGAAACAAAGTTATTGGAAAACATAAGCGCCAGTCTAGACAGCTCAAGGCTGTTTTTTAGC
CATTGCAAAGGAATAAAAACCCACTCTCAGGAGTGTCCTAGAACAATTCTGGAGCAGCCAATCACTACAGTGAATATCCATCGCTTCTTGAAACTTATGG
ATGCACCTCTTGTGATGGACATTTCTGGAGATCCTGAAAAAGTTACAAGTGTTCACCTGCAACTTGGCTTGCCGATGGTTTTTATTCTCAGTCAGCAAGA
AACCTACGAGTTTGATAGAACAACCGCAGAACATGTGGCCTGGCAGCTGTTGGGAAAAGCAGGGATAGGGATACTACTGAGGGAAAAAGCTCATTCATCA
GTTCCAACAAATTGTAATGTCGCAGTGAAAAGACCAAACGAGGACAGTCCCGTGCAATACATGACGATGGAAGAAACCCAAGAAATTAGAAATATTATTG
AAAAGTCTAACATGGAGCAGTATAACAAATCTGAAGAGACAAGTGTCTTTGCTACTCAAGAGATTCAAGATGACGAAGTTGCAGAAGCTGTGTATAGAGA
CAGAAAGCGGACCCTGCCCCTTCATCTAGTGCCCTCGTTAACAGATGAAAATTTCAAGCATGTGTTGACAAACCCCCCTATGTCAATGATTTTGTACTAT
GCAAGCTGGGAAGCAGTATCATTGACTTTACTGCAGACATTTGTTCATATGGCTGAAAAGTATAAAGATACACTGGATATGATTTTAGCCAGAGTGAACT
GTGCAGATTGGCCAAATATTTGCAGTGAGCAAAATGTTAGTAACATCCCGGTGGTAAAGATCTACCAAATGGGGAAAGAGCCATTGGAATACACTGGTAT
GCTGGGCGCTGAGGAGCTATTCAGATTTAGTATGCTAGCCAAGGTAGACTGCCCTTTAGAGTTGTTTTCTTATGAGGAGGCAGAACAGTTTCTCAGTGGA
AAGCTTAATCAGTTCTTGCTACCTTATCATAATCTTTCAGTGTTGGGAATCTTTACCAAAAATACAAAAGAAGTTGATTCATATTTTAATGCTGGCAAAA
GTCTGTGGGGCTTTGCAAGCTTGGGAATTTACTATGAAGATAATGCCATGATCATGTCAAAAAAATATGGCATAACTCCCCCTGCTCTACTTTTTGCTAG
GCATGACACTAACCAAGTACACAGTGCTACTCTCCAGCATACTGCCGAAAGGGATATCATTAACACTGTCAGGATGGAATTACTCAGAGAGTTTCCAGAA
ATCACAATGGAAAATTTCCCAACATTCTTCATGCAAAAAAAGCCTTTATTGGTTTTATTTTCCAATTGTAACCCAACCCAGAGTGATGAAAAACACATTT
TGAACTTGGTGAGAGGAAGGTATCTGGATCAGTACTTGGCTTGCTGGCTAAACCTGAAGAATACTCCAGTGGGCGTTGGAATACTGCAGCGGTACTTTGG
CATAGTACCAAGTTTACCACAACTTGTTCTTATTGATTTTGATTCCTTAGGTCAGGTCTTCTCATTTCCTTTAGATCACCATCTTACTGATGTAAATATT
TTGTATTGGTTGGAGATGATAAAAGCAGGAGCGGAATTGCCTGTCTATAGTTTATCCAATGAAGACTGGAAACCACCACTTCCCGATTACAATTTCCTTG
ATGTGATGGATGCAGAAGTCCCAGGCTTTGCTGCACAGAAGATACGTATTCAAATGAAGCCCAACAGAAGGCAAAAAGTCGAAGATTCTCCAGAGGGAGT
TGAAGAAGAAAGCGAAGCCAAGAAGTATCCTCCAGGCTCAAGTTTACGTGGAACTGTGCCAAAGTTTATAGAACAGGGAAAAAAGTTCAGAAGTCATACA
GAGTTATGATCTGAGTGGTGTGTGAAACACAAAATAAATTGTGGAAAATCCCTAAAAAAAAAAAAAAAAAA
5' EST: CX841792 [+]gaataggatgctttcagaggaagactggccgcagagtatggaccttaacctgggatggctgtaaatctgtgcagctatgccatgtcttcaagaacctgct
aattagaatcctgagcagacactgaacaggctcatacctctttcatgctttcagatgaacaatcttttttagggcatgagcaaattgctggtccagataa
gtagtctcagtcaccttggtgaaatcacttgaagatatggtgattccagctgatccaaattaaaataccttttaaattaaaatggctggcctgatccgtt
tagctacttttttgtgtgtaaatagtatttttatgacaaaggcaataaatactttggatgaactaagatcagatacctattatagtgcccttatgcctgg
gaagacatcactactttatttcactaatccagcttttcctagtaatattattttccttgatgaactccaaaagtctgctggggctcttcaagactatggg
atttccgtcgctaaggttaattgtatgaaagaagacctactaaagtactgtggggaggagaatgcatatttgtttaggggtgctaagttagtaagggaat
ttccaacagatgcactttttgatgttgattctatcgtggccaatgtcttatttgttctcctttataatgaagttaaatacataaccacctctatggagca
ccaaaatattgaaagtgctatgaaagggaaaaaagacttaatttttgcctacngttcagcaataggaataccagaacatagatcggttatggagactgcg
tttgtttatggatcaaaataccaatttattt
3' EST: CX841791 [+]agggattttccacaatttattttgtgtttcacacaccactcagatcataactctgtatgacttctgaacttttttccctgttctataaactttggcacag
ttccacgtaaacttgagcctggaggatacttcttggcttcgctttcttcttcaactccctctggagaatcttcgactttttgccttctgttgggcttcat
ttgaatacgtatcttctgtgcagcaaagcctgggacttctgcatccatcacatcaaggaaattgtaatcgggaagtggtggtttccagtcttcattggat
aaactatagacaggcaattccgctcctgcttttatcatctccaaccaatacaaaatatttacatcagtaagatggtgatctaaaggaaatgagaagacct
gacctaaggaatcaaaatcaataagaacaagttgtggtaaacttggtactatgccaaagtaccgctgcagtattccaacgcccactggagtattcttcag
gtttagccagcaagccaagtactgatccagataccttcctctcaccaagttcaaaatgtgtttttcatcactctgggttgggttacaattggaaaataaa
accaataaaggctttttttgcatgaagaatgttgggaaattttccattgtgatttctggaaactctctgagtaattccatcctgacagtgttaatgatat
ccctttcggcagtatgctggagagtagcactgtgtacttggttagtgtcatgcctagcaaaaagtagagcagggggagttatgccatatttttttgacat
gatcatggcattatcttcatagtaaattcccaagcttgcaaagc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: