XB-CLONE-3638326
Clone Name: IMAGE:7661757
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Gene:
large2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC136204 [+]
GTGGGATTTAACCAGTGTTATGGTGGAACCAGTGCCCCAGGCAGGGGGATACAGGAGCCGATCCTCCGGCTGGCTGGAGTCAAAATGTGGGGCTGCCTGG
TGCCTCTGATGCCGGAATAGAGTGAGTTTGGAGCATTAACCAGCACTAAACCCTCCCCCAGCAATCTGGAGACTGCCCGAGAGACTGCCCGGGTCTGAAG
TTACCTGCAACCGCTGGATGTGCCCTATATAAACCGCCGCTGGATGGAAACTTAGCAGACACTTCGCCTGCTTCTGGGCATTGTGGTGCCACTTTAAAGC
TTATAGGATGTTGCCGGCAGTAATACCAGACTTTCCCACTTGACGGCCCCCCAACGACGCAGTCCAGGACAGTGTGGACCCATCATGCACCTCTCCTGTC
GCAGCAAAGCCAAGTTCCTGGCCCTCGCTTTACTGCTGCTGTCTGCTGCAACCTGGTTGTACCTGTCTGTGGGGGAGACAGAATATGGTCCATCCCTGGG
GCCGGTAGCCCCTCATTTTTCGGCCACACCGTCCCTCCAGTTAGAGCTGGAGAGCCGCCTGCGGGTAGCTGAGGAGGAGAATCGGAGACTGCGCCAGCAA
CTGGGCGAGATGAGGAATGAGGAGCAAAGCACCGGAGTAGGGACGGAAGGGGACAACAGCAGCCATTGTGAGCACAGGAGCCTGACTGAGAAGTGTGAAC
TGATCCACGTGGCCATAGTCTGCGCCGGCCATAACAGCAGCCGAGATGTGGTGACCCTGGTGAAGTCCATTCTGTTCCACAGGAGAAACCCCTTACATCT
GCACCTGATCACGGACGCCGTGGCTCTGCGCGTGCTGGGGAACCTGTTCAAGACCTGGATGGTGCCGTCCCTACAAATCAGCTTCTATAACGCAAGTGAG
CTGAAGCCGGACGTTGCGTGGATCCCAAACAAACATTACTCGGGGATATATGGGTTACTGAAGCTCACACTGACCAAGGCGCTGCCCTCCGACCTCTCCA
AGGTCATCGTCCTGGACACGGACATCACGTTTGCCACCGACATTGCCGAGCTCTGGGCAATATTTAAGAAGTTCACAGGTGAGCAAGTTCTGGGTCTGGT
GGAGAATCAGAGTGACTGGTACCTGGGGAACCTGTGGAAGAACCACAAGCCGTGGCCTGCCTTGGGCCGAGGATTCAACACAGGGGTCATTCTGCTGCTG
TTGGACAAGCTGCGCCTGATTGGCTGGGAGGAGATGTGGCGACTGACAGCGGAGAGGGAGTTGATGAATATGCTGAGCACCTCGCTGGCTGACCAGGATA
TATTCAATGCTGTTATCAAGAGTTCCCCTACTCTAGTCTATCAGCTGCCCTGTTACTGGAACGTGCAGCTCTCCGACCACACGCGCTCCGAGCAGTGTTA
CAGCGAGCTGGCCGACCTCAAGGTCATTCACTGGAACTCCCCCCACAAGCTGCGAGTCAAGAACAAGCACGTTGAACTATTCCGCACCCTGTACCTGACT
TTCCTAGAGTACGACGGGAGCCTCCTGCGCCGAGAGCTGATTGGCTGTCCGAGTGAAGGGGAACAGCAGGGGGGCAGCCAGGCTGCTCTGTCGCAATTGG
ACGAGGAAGATCCGTGCTATGATTTCCGGAGGGAGAGCCTTGCATCCCACCGCGTCCACCTGTCCTTCCTGCCGCACGTAACCCCGCCCCCGGACCCCTA
TGATGTCACGCTGGTGGCACAGCTCTCTATGGACAGGCTGCAAATGCTGGAGCTGATCTGCCGGCACTGGGACGGCCCAATGAGCCTGGCCCTGTACCTG
TCGGACGCGGAGGCGCAGCAGTTCCTACGCTATGCCCAGGCCTCCGAGGTGCTGCAGTCTCGCACCAACGTCGCCTACCATGTGGTCTATAAGGAGGGGC
AGCTGTACCCCGTCAACCTCCTGCGCAACGTGGCCCTGAAGAACTCACAGACCCCTTACGTCTTCCTGTCGGATATTGATTTCCTGCCCATGTACGGACT
CTATGAATACCTACGGAAATCCATATCCCAGCAGGACCTGACCGGCCCCCCCAAGGCTCTTATCGTTCCCGCCTTTGAGACTCTCCGTTACCGGCTTTCT
TTCCCCAAGTCCAAGGCAGAGCTACTCTCCATGCTGGATACGGGGGCGCTCTACACCTTCAGGTACCACGTATGGGAGAAAGGTCACGCCCCCACAGATT
ATGCCAAGTGGAGGACTGCGACCACCCCGTACCGGGTGGAGTGGGCGCCAGACTTCGAGCCGTACGTAGTGGTGAGACGGGACTGCCCTGAGTATGACCA
GCGCTTCCTCGGCTTTGGCTGGAACAAAGTGTCGCACATCATGGAGCTGGATGCCCAGGAACATGAACTGCTGGTTCTGCCTAATGCCTTTATCATCCAC
ATGCCCCACGCTCCCAGCTTCGACATCTCCAAGTTCCGCTCCAGCGAAAATTACCGCCTGTGCGTCCAGGCCCTTAAGGAGGAGTTTCACCAAGACTTGT
CCCGGCGCTACGGTTCCGCTGCCCTAAAATACCTCACGGCTGAGCGCAACCGGTGAACTTGGTGCAGAGACGCTGCCAGGAAACACTACAGAGAGGGGTG
TGAGGACAATGGGCTCTTCCGTGCCTCTGCTCTTCTACATACCTGCCTTATACAGGGCCTTCATTATAGGAGGTGCCTGGCCCTTTAATAGGAACGAACC
AGTTGCCACGGGGGATGTTATTTCTGTGGCCTTTGTATGGGCCACCGGACAATCAGCTGCAGTTCCCCCGGCCAATGGGGACGCGAGTCGTGATCACTGG
ACGGAGAGGGTCTCTCCCTGACGCATTTCAATATTTCTCTTTATTTATATTATTTATGTGGATAATTCCGATGAAATCAAACTGACAGGGCAGGATTCCT
GATTCTATTTTTAAAATATATTCAGTTTCTTTTTGCAATAAAATGCTGCAATCGTTAAAAAAAAAAAAAAAAAA
5' EST: CX843012 [+]tgggatttaaccagtgttatggtggaaccagtgccccaggcagggggatacaggagccgatcctccggctggctggagtcaaaatgtggggctgcctggt
gcctctgatgccggaatagagtgagtttggagcattaaccagcactaaaccctcccccagcaatctggagactgcccgagagactgcccgggtctgaagt
tacctgcaaccgctggatgtgccctatataaaccgccgctggatggaaacttagcagacacttcgcctgcttctgggcattgtggtgccactttaaagct
tataggatgttgccggcagtaataccagactttcccacttgacggccccccaacgacgcagtccaggacagtgtggacccatcatgcacctctcctgtcg
cagcaaagccaagttcctggccctcgctttactgctgctgtctgctgcaacctggttgtacctgtctgtgggggagacagaatatggtccatccctgggg
ccggtagcccctcatttttcggccacaccgtccctccagttagagctggagagccgcctgcgggtagctgaggaggagaatcggagactgcgccagcaac
tgggcgagatgaggaatgaggagcaaagcaccggagtagggacggaaggggacaacagcagccattgtgagcacaggagcctgactgagaagtgtgaact
gatccacgtggccatagtctgcgccggccataacagcagccgagatgtggtgaccctggtgaagtccattctgttccacaggagaaaccccttacatctg
cacctgatcacggacgccgtggctctgcgcgtgctgg
3' EST: CX843011 [+]aacgattgcagcattttattgcaaaaagaaactgaatatattttaaaaatagaatcaggaatcctgccctgtcagtttgatttcatcggaattatccaca
taaataatataaataaagagaaatattgaaatgcgtcagggagagaccctctccgtccagtgatcacgactcgcgtccccattggccgggggaactgcag
ctgattgtccggtggcccatacaaaggccacagaaataacatcccccgtggcaactggttcgttcctattaaagggccaggcacctcctataatgaaggc
cctgtataaggcaggtatgtagaagagcagaggcacggaagagcccattgtcctcacacccctctctgtagtgtttcctggcagcgtctctgcaccaagt
tcaccggttgcgctcagccgtgaggtattttagggcagcggaaccgtagcgccgggacaagtcttggtgaaactcctccttaagggcctggacgcacagg
cggtaattttcgctggagcggaacttggagatgtcgaagctgggagcgtggggcatgtggatgataaaggcattaggcagaaccagcagttcatgttcct
gggcatccagctccatgatgtgcgacactttgttccagccaaagccgaggaagcgctggtcatactcagggcagtcccgtctcaccactacgtacggctc
gaagtctggcgcccactccacccggtacggggtggtcgcagtcctccacttggcataatctgtgggggcgtgacctttctcccatacgtggtacctgaag
gtgtagagcgcccccgtatccagcatggagagtagctctgccttggac
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 1.5-2 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: brain | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: